Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of Solanum nigrum L. Expressing Iron-Regulated Transporter Gene IRT1

Solanum nigrum L., a hyperaccumulator of cadmium (Cd), is regarded as a promising candidate for phytoremediation of heavy metal pollution. In the present study, the hairy roots of Solanum nigrum L. were selected as a model plant system to study the potential application of Iron-regulated Transporter Gene (IRT1) for the efficient phytoremediation of Cd pollution. The transgenic hairy roots of Solanum nigrum L. expressing the IRT1 gene from Arabidopsis thaliana were successfully obtained via the Agrobacterium tumegaciens-mediated method. Expression of IRT1 reduced Cd stress-induced phytotoxic effects. Significantly superior root growth, increased antioxidant enzyme activities, decreased reactive oxygen species (ROS) levels, and less cell apoptosis were observed in the transgenic hairy roots of Solanum nigrum L. compared to the wild-type lines under Cd stress. Enhanced Cd accumulation was also carried out in the transgenic hairy roots compared to the control (886.8 μg/g vs. 745.0 μg/g). These results provide an important understanding of the Cd tolerance mechanism of transgenic IRT1 hairy roots of Solanum nigrum L., and are of particular importance to the development of a transgenic candidate for efficient phytoremediation process.

[1]  Yong Wang,et al.  The molecular mechanism underlying cadmium resistance in NHX1 transgenic Lemna turonifera was studied by comparative transcriptome analysis , 2020, Plant Cell, Tissue and Organ Culture (PCTOC).

[2]  M. Greger,et al.  Screening the Capacity of 34 Wetland Plant Species to Remove Heavy Metals from Water , 2020, International journal of environmental research and public health.

[3]  K. Rogers,et al.  A case of milk traceability in small-scale districts-Inner Mongolia of China by nutritional and geographical parameters. , 2020, Food chemistry.

[4]  Gang Wu,et al.  Regulation of cadmium tolerance and accumulation by miR156 in Arabidopsis. , 2020, Chemosphere.

[5]  Yaping Sun,et al.  Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress , 2019, International journal of molecular sciences.

[6]  Long Yang,et al.  Research on Progress in Combined Remediation Technologies of Heavy Metal Polluted Sediment , 2019, International journal of environmental research and public health.

[7]  Xiaohui Ji,et al.  Phytoremediation of Heavy Metal Pollution: A Bibliometric and Scientometric Analysis from 1989 to 2018 , 2019, International journal of environmental research and public health.

[8]  R. Azevedo,et al.  Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance , 2019, Environmental Science and Pollution Research.

[9]  S. Moon,et al.  Cytoprotective effects of taxifolin against cadmium-induced apoptosis in human keratinocytes , 2019, Human & experimental toxicology.

[10]  F. Napolitano,et al.  Recent Updates on the Use of Agro-Food Waste for Biogas Production , 2019, Applied Sciences.

[11]  Jichen Xu,et al.  Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport. , 2018, International journal of biological macromolecules.

[12]  Xing Fan,et al.  Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants , 2018, Planta.

[13]  Z. Xiang,et al.  Two mulberry phytochelatin synthase genes confer zinc/cadmium tolerance and accumulation in transgenic Arabidopsis and tobacco. , 2018, Gene.

[14]  Q. Yan,et al.  Biochemical properties and application of a novel β-1,3-1,4-glucanase from Paenibacillus barengoltzii. , 2017, Food chemistry.

[15]  Saifullah,et al.  Remediation of heavy metal contaminated soils by using Solanum nigrum: A review. , 2017, Ecotoxicology and environmental safety.

[16]  E. Wieczorek,et al.  Cadmium, arsenic, selenium and iron- Implications for tumor progression in breast cancer. , 2017, Environmental toxicology and pharmacology.

[17]  Zhengqiang Jiang,et al.  Characterization of actinidin from Chinese kiwifruit cultivars and its applications in meat tenderization and production of angiotensin I-converting enzyme (ACE) inhibitory peptides , 2017 .

[18]  T. Macek,et al.  Transgenic plants and hairy roots: exploiting the potential of plant species to remediate contaminants. , 2016, New biotechnology.

[19]  Surajit Bhattacharya,et al.  Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene OsMTP1 is promising for phytoremediation. , 2016, Plant physiology and biochemistry : PPB.

[20]  Jian-gang Yuan,et al.  Comparative Transcriptome Analysis between Low- and High-Cadmium-Accumulating Genotypes of Pakchoi (Brassica chinensis L.) in Response to Cadmium Stress. , 2016, Environmental science & technology.

[21]  Liping Xia,et al.  Cadmium induced oxidative damage and apoptosis in the hepatopancreas of Meretrix meretrix , 2016, Ecotoxicology.

[22]  Chuanping Yang,et al.  Overexpression of ThVHAc1 and its potential upstream regulator, ThWRKY7, improved plant tolerance of Cadmium stress , 2016, Scientific Reports.

[23]  G. Rödel,et al.  The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity , 2015, Front. Plant Sci..

[24]  Xin Sun,et al.  Cadmium contamination of rice from various polluted areas of China and its potential risks to human health , 2015, Environmental Monitoring and Assessment.

[25]  Shuang Li,et al.  Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds , 2014, Transgenic Research.

[26]  Shanshan Wang,et al.  Hyperaccumulative property of Solanum nigrum L. to Cd explored from cell membrane permeability, subcellular distribution, and chemical form , 2014, Journal of Soils and Sediments.

[27]  L. Kochian,et al.  Targeted expression of SbMATE in the root distal transition zone is responsible for sorghum aluminum resistance. , 2013, The Plant journal : for cell and molecular biology.

[28]  J. K. Kim,et al.  Enhanced accumulation of phytosterol and triterpene in hairy root cultures of Platycodon grandiflorum by overexpression of Panax ginseng 3-hydroxy-3-methylglutaryl-coenzyme A reductase. , 2013, Journal of agricultural and food chemistry.

[29]  P. Tsang,et al.  Effect of cadmium on cytogenetic toxicity in hairy roots of Wedelia trilobata L. and their alleviation by exogenous CaCl2 , 2013, Environmental Science and Pollution Research.

[30]  Jianhang Sun,et al.  Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum. , 2012, The New phytologist.

[31]  C. Curie,et al.  Monoubiquitin-dependent endocytosis of the IRON-REGULATED TRANSPORTER 1 (IRT1) transporter controls iron uptake in plants , 2011, Proceedings of the National Academy of Sciences.

[32]  S. Eapen,et al.  Glutathione Transferase from Trichoderma virens Enhances Cadmium Tolerance without Enhancing Its Accumulation in Transgenic Nicotiana tabacum , 2011, PloS one.

[33]  A. Schulz,et al.  Monitoring reactive oxygen species formation and localisation in living cells by use of the fluorescent probe CM-H(2)DCFDA and confocal laser microscopy. , 2009, Physiologia plantarum.

[34]  Qixing Zhou,et al.  Antioxidative defense and proline/phytochelatin accumulation in a newly discovered Cd-hyperaccumulator, Solanum nigrum L. , 2007 .

[35]  Qixing Zhou,et al.  A newly-discovered Cd-hyperaccumulator Solatium nigrum L. , 2005 .

[36]  P. Doran,et al.  Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens. , 2003, Biotechnology and bioengineering.

[37]  M. Guerinot,et al.  Expression of the IRT1 Metal Transporter Is Controlled by Metals at the Levels of Transcript and Protein Accumulation Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001263. , 2002, The Plant Cell Online.

[38]  T. Iwashita,et al.  Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves) , 1997, Plant physiology.

[39]  D. Eide,et al.  A novel iron-regulated metal transporter from plants identified by functional expression in yeast. , 1996, Proceedings of the National Academy of Sciences of the United States of America.