Antimicrobial susceptibility of Salmonella isolated from various products, from 1999 to 2003.

Foodborne salmonellosis continues to be a major health concern worldwide; thus, detection and tracking of antimicrobial resistance in Salmonella isolates is of interest. The U.S. Food and Drug Administration initiated antimicrobial sensitivity screening of Salmonella isolates from food and related samples in 1999. This paper summarizes the antimicrobial resistance data for Salmonella isolates obtained from 1999 to 2003. A total of 22,231 imported and domestic samples were analyzed for Salmonella, of which 1,319 (5.9%) yielded the pathogen. Since more than one culture was isolated from some samples, the total number of isolates obtained and tested for antimicrobial sensitivity was 1,382. Antimicrobial sensitivity screening was performed with the disc diffusion assay on 11 antimicrobial agents. Of the 1,108 food isolates screened, 42.1% (n = 467) were serotypes Weltevreden, Newport, Lexington, Senftenberg, Typhimurium, Saint Paul, Paratyphi, Enteritidis, Thompson, and Bareilly. A total of 249 (18.0%) isolates from all sources were resistant to two or more antimicrobials. Resistance to sulfisoxazole, streptomycin, and tetracycline was most common, whereas resistance to ciprofloxacin was least common. Weltevreden (n = 148) was the most common serotype isolated from food, but only nine (6.1%) of these isolates were resistant to two or more antimicrobials. In contrast, although Derby was recovered only 19 times, 11 (57.9 %) of these isolates were resistant to two or more antimicrobials. Of the 274 isolates from animal feed, dog treats and environmental swabs, 49.6% (n = 136) belonged to serotypes Infantis, Mbandaka, Anatum, Senftenberg, Typhimurium, Montevideo, Cerro, Enteritidis, and Bredeney, with 76 (27.7%) of these isolates resistant to two or more antimicrobials. Only limited trends in antimicrobial resistance were observed over time, with resistance to sulfisoxazole increasing, resistance to tetracycline decreasing, and resistance to streptomycin fluctuating.

[1]  J. Sofos,et al.  Characterization of antimicrobial-resistant Salmonella isolated from imported foods. , 2006, Journal of food protection.

[2]  J. Meng,et al.  Prevalence and Antimicrobial Resistance of Campylobacter spp. and Salmonella Serovars in Organic Chickens from Maryland Retail Stores , 2005, Applied and Environmental Microbiology.

[3]  C. Doetkott,et al.  Comparison of antimicrobial resistance in Salmonella and Campylobacter isolated from turkeys in the Midwest USA , 2004 .

[4]  F. Smulders,et al.  Antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry. , 2004, International journal of food microbiology.

[5]  I. Wilson.  Antimicrobial resistance of Salmonella in raw retail chickens, imported chicken portions, and human clinical specimens. , 2004, Journal of food protection.

[6]  W. van Pelt,et al.  Antimicrobial Susceptibilities of Salmonella Strains Isolated from Humans, Cattle, Pigs, and Chickens in The Netherlands from 1984 to 2001 , 2003, Journal of Clinical Microbiology.

[7]  Shaohua Zhao,et al.  Antimicrobial-resistant Salmonella serovars isolated from imported foods. , 2003, International journal of food microbiology.

[8]  E. Threlfall.  Antimicrobial drug resistance in Salmonella: problems and perspectives in food- and water-borne infections. , 2002, FEMS microbiology reviews.

[9]  J. Sofos,et al.  Antimicrobial resistance of food-related Salmonella isolates, 1999-2000. , 2002, Journal of food protection.

[10]  D. Low,et al.  The emergence and spread of antibiotic resistance in food-borne bacteria. , 2000, International journal of food microbiology.

[11]  L. McCaig,et al.  Food-related illness and death in the United States. , 1999, Emerging infectious diseases.

[12]  H. Herikstad,et al.  Emerging quinolone-resistant Salmonella in the United States. , 1997, Emerging infectious diseases.

[13]  A. Brisabois,et al.  Surveillance of antibiotic resistance in Salmonella. , 1997, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[14]  D. E. Bradley,et al.  Apramycin and gentamicin resistance in Escherichia coli and salmonellas isolated from farm animals , 1986, Journal of Hygiene.

[15]  L R Ward,et al.  Increasing incidence of resistance to gentamicin and related aminoglycosides in Salmonella typhimurium phage type 204c in England, Wales and Scotland , 1985, Veterinary Record.

[16]  S. Holmberg,et al.  Drug-resistant Salmonella from animals fed antimicrobials. , 1984, The New England journal of medicine.

[17]  E. Threlfall,et al.  International spread of multiresistent strains of Salmonella typhimurium phage types 204 and 193 from Britain to Europe , 1979, Veterinary Record.

[18]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[19]  A. Brandelli,et al.  Antimicrobial resistance in Salmonella enteritidis strains isolated from broiler carcasses, food, human and poultry-related samples. , 2005, International journal of food microbiology.

[20]  R. Tauxe,et al.  Origins and consequences of antimicrobial-resistant nontyphoidal Salmonella: implications for the use of fluoroquinolones in food animals. , 2000, Microbial drug resistance.

[21]  E. Threlfall,et al.  Increase in multiple antibiotic resistance in nontyphoidal salmonellas from humans in England and Wales: a comparison of data for 1994 and 1996. , 1997, Microbial drug resistance.