Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes.

Ten bronchoscopes that had been used on patients with the acquired immunodeficiency syndrome were sampled to determine the nature and extent of microbial contamination. Samples were taken by irrigating the suction biopsy channel with modified viral transport medium and by swabbing the insertion tube. Sampling was repeated after they had been cleaned in detergent and after two minutes' disinfection in 2% alkaline glutaraldehyde. Before being cleaned the seven bronchoscopes tested by polymerase chain reaction were contaminated with the human immunodeficiency virus, though infectivity and antigen assays gave negative results. Other organisms identified were hepatitis B virus (1), commensal bacteria (9), and Pneumocystis carinii (4). Mean bacterial contamination was 2.27 log colony forming organisms per millilitre. Cleaning the bronchoscope before disinfection removed all detectable contaminants with a reduction in bacterial growth of up to 8 log colony forming units/ml.

[1]  J. Armstrong,et al.  Mycobacterial cross-contamination of bronchoscopy specimens. , 2015, The American review of respiratory disease.

[2]  A. Collier,et al.  Plasma viremia in human immunodeficiency virus infection. , 1989, The New England journal of medicine.

[3]  D. Jeffries,et al.  CONTAMINATION OF ENDOSCOPES USED IN AIDS PATIENTS , 1989, The Lancet.

[4]  D. Jeffries,et al.  Chemical inactivation of HIV on surfaces. , 1989, BMJ.

[5]  K. A. Fleming,et al.  FALSE-POSITIVE RESULTS AND THE POLYMERASE CHAIN REACTION , 1988, The Lancet.

[6]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[7]  L. Resnick,et al.  Detection of HTLV-III/LAV-specific IgG and antigen in bronchoalveolar lavage fluid from two patients with lymphocytic interstitial pneumonitis associated with AIDS-related complex. , 1987, The American journal of medicine.

[8]  L. Epstein,et al.  Detection of HTLV-III RNA in lungs of patients with AIDS and pulmonary involvement. , 1986, JAMA.

[9]  J. Groopman,et al.  Human T lymphotropic virus type III infection of human alveolar macrophages. , 1986, Blood.

[10]  L. Resnick,et al.  Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. , 1986, JAMA.

[11]  E. Abrutyn,et al.  Pseudo-outbreak of Bacillus species: related to fibreoptic bronchoscopy. , 1985, The Journal of hospital infection.

[12]  A. Hanwell.  THE IMMERSIBLE ENDOSCOPE , 1983, The Lancet.

[13]  E. Quigley,et al.  Endoscopic transmission of hepatitis B virus. , 1983, Gut.

[14]  R. Israel,et al.  Pseudomonas aeruginosa contamination of fibreoptic bronchoscopes. , 1982, The Journal of hospital infection.

[15]  Leers Wd.  Disinfecting endoscopes: how not to transmit Mycobacterium tuberculosis by bronchoscopy. , 1980 .

[16]  S. A. Hussain.  Fiberoptic bronchoscope-related outbreak of infection with Pseudomonas. , 1978, Chest.

[17]  R. Bone,et al.  Contamination of a fiberoptic bronchoscope with Proteus species. , 1977, The American review of respiratory disease.

[18]  S. Webb,et al.  Outbreak of Serratia Marcescens Associated with the Flexible Fiberbronchoscope , 1975 .

[19]  K. Nelson,et al.  Transmission of tuberculosis by flexible fiberbronchoscopes. , 1983, The American review of respiratory disease.

[20]  W. Leers.  Disinfecting endoscopes: how not to transmit Mycobacterium tuberculosis by bronchoscopy. , 1980, Canadian Medical Association journal.

[21]  R C Elliott,et al.  Complications of fiberoptic bronchoscopy. , 1974, The American review of respiratory disease.