Hypoglycemic effect of saponin extracts from leaves of Momordica charantia

In order to investigate hypoglycemic effects of Momordica charantia(MC) leaves saponins, saponins with purity of 86.0% were extracted from leaves of MC. The extracts were used to treat hyperglycemic rats and the damaged insulinoma cell RIN-M5F in vitro. The effects of MC leaves extracts on the metabolic parameters (serum glucose, insulin, glycogen, Superoxide Dismutase(SOD) activity and Malonedialdehyde (MDA) levels of hyperglycemic rats and the effects on RIN-M5F cells were observed. The results showed that there was a significant increase in serum insulin and glycogen, accompanied by a decrease in serum glucose and MDA in treated rats with MC leaves saponins. And the extracts also exhibited obvious protection and repairation effects on RIN-M5F cells.

[1]  T. Ng,et al.  Bitter gourd (Momordica charantia) is a cornucopia of health: a review of its credited antidiabetic, anti-HIV, and antitumor properties. , 2011, Current molecular medicine.

[2]  Y. Kuo,et al.  Isolation and identification of cucurbitane-type triterpenoids with partial agonist/antagonist potential for estrogen receptors from Momordica charantia. , 2011, Journal of agricultural and food chemistry.

[3]  M. Biyani,et al.  Antihyperglycemic effects of three extracts from Momordica charantia. , 2003, Journal of ethnopharmacology.

[4]  A. Nussler,et al.  Effect of Momordica grosvenori on oxidative stress pathways in renal mitochondria of normal and alloxan-induced diabetic mice , 2007, European journal of nutrition.

[5]  R. Bhonde,et al.  Role of bittergourd fruit juice in stz-induced diabetic state in vivo and in vitro. , 2000, Journal of ethnopharmacology.

[6]  P. Chaturvedi,et al.  Momordica charantia maintains normal glucose levels and lipid profiles and prevents oxidative stress in diabetic rats subjected to chronic sucrose load. , 2010, Journal of medicinal food.

[7]  H. Raza,et al.  Tissue specific expression and immunohistochemical localization of glutathione S-transferase in streptozotocin induced diabetic rats: modulation by Momordica charantia (karela) extract. , 2004, Life sciences.

[8]  G. Olayiwola,et al.  Hypoglycaemic and hypotensive effects of Momordica charantia Linn (Cucurbitaceae) whole-plant aqueous extract in rats. , 2006, Cardiovascular journal of South Africa : official journal for Southern Africa Cardiac Society [and] South African Society of Cardiac Practitioners.

[9]  Jaipaul Singh,et al.  Effects of Momordica charantia fruit juice on islet morphology in the pancreas of the streptozotocin-diabetic rat. , 1998, Diabetes research and clinical practice.

[10]  H. Matsuda,et al.  Structures of new cucurbitane-type triterpenes and glycosides, karavilagenins and karavilosides, from the dried fruit of Momordica charantia L. in Sri Lanka. , 2006, Chemical & pharmaceutical bulletin.

[11]  H. Matsuda,et al.  Medicinal foodstuffs. XXI. Structures of new cucurbitane-type triterpene glycosides, goyaglycosides-a, -b, -c, -d, -e, -f, -g, and -h, and new oleanane-type triterpene saponins, goyasaponins I, II, and III, from the fresh fruit of Japanese Momordica charantia L. , 2001, Chemical & pharmaceutical bulletin.

[12]  Y. Goh,et al.  Effects of Momordica charantia on pancreatic histopathological changes associated with streptozotocin-induced diabetes in neonatal rats. , 2011, Histology and histopathology.

[13]  Y. Goh,et al.  Effects of Momordica charantia aqueous extract on renal histopathological changes associated with streptozotocin- induced diabetes mellitus type II in neonatal rats , 2011 .

[14]  Pornsiri Pitchakarn,et al.  Induction of G1 arrest and apoptosis in androgen-dependent human prostate cancer by Kuguacin J, a triterpenoid from Momordica charantia leaf. , 2011, Cancer letters.

[15]  M. Krawinkel,et al.  Antidiabetic effects of bitter gourd extracts in insulin-resistant db/db mice , 2010, British Journal of Nutrition.

[16]  H. Tokuda,et al.  Cucurbitane-type triterpenoids from the fruit of Momordica charantia. , 2005, Journal of natural products.

[17]  S. K. Tongia,et al.  Phytochemical determination and extraction of Momordica charantia fruit and its hypoglycemic potentiation of oral hypoglycemic drugs in diabetes mellitus (NIDDM). , 2004, Indian journal of physiology and pharmacology.