General and molecular microbiology and microbial genetics in the IM CAS

[1]  Pavel Kyslík,et al.  The impact of Ivan Málek’s continuous culture concept on bioprocessing , 2010, Journal of Industrial Microbiology & Biotechnology.

[2]  L. Váchová,et al.  Yeast Colony Survival Depends on Metabolic Adaptation and Cell Differentiation Rather Than on Stress Defense* , 2009, The Journal of Biological Chemistry.

[3]  R. Benz,et al.  Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin , 2009, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[4]  D. Pieper,et al.  Metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation , 2009, Environmental microbiology.

[5]  L. Kubínová,et al.  Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter. , 2009, Environmental microbiology.

[6]  P. Ivanov,et al.  Robust heat shock induces eIF2α-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae , 2009, Journal of Cell Science.

[7]  J. Vohradský,et al.  Inference of active transcriptional networks by integration of gene expression kinetics modeling and multisource data. , 2009, Genomics.

[8]  K. Sigler,et al.  Characterization of the kinetics and mechanisms of inhibition of drugs interacting with the S. cerevisiae multidrug resistance pumps Pdr5p and Snq2p. , 2009, Biochimica et biophysica acta.

[9]  P. Vašicová,et al.  Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae , 2009, Eukaryotic Cell.

[10]  M. Pátek,et al.  Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation. , 2009, Journal of biotechnology.

[11]  F. Devaux,et al.  Metabolic diversification of cells during the development of yeast colonies. , 2009, Environmental microbiology.

[12]  C. Dekker,et al.  The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I , 2008, Journal of molecular biology.

[13]  W. Frommer,et al.  Plasma membrane microdomains regulate turnover of transport proteins in yeast , 2008, The Journal of cell biology.

[14]  O. Benada,et al.  Adenylate Cyclase Toxin Subverts Phagocyte Function by RhoA Inhibition and Unproductive Ruffling1 , 2008, The Journal of Immunology.

[15]  I. Linhartova,et al.  Single‐step affinity purification of recombinant proteins using a self‐excising module from Neisseria meningitidis FrpC , 2008, Protein science : a publication of the Protein Society.

[16]  A. Hinnebusch,et al.  eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA. , 2008, Genes & development.

[17]  M. Weiserová,et al.  Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31) , 2008, BMC Microbiology.

[18]  J. Vohradský,et al.  Effect of protein degradation on spot Mr distribution in 2‐D gels – a case study of proteolysis during development of Streptomyces coelicolor cultures , 2008, Proteomics.

[19]  M. Szczelkun,et al.  A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I , 2008, Nucleic acids research.

[20]  J. Vohradský,et al.  Biocomputational prediction of small non-coding RNAs in Streptomyces , 2008, BMC Genomics.

[21]  J. Mašín,et al.  RTX cytotoxins recognize β2 integrin receptors through N-linked oligosaccharides , 2008, Proceedings of the National Academy of Sciences.

[22]  P. Halada,et al.  SsrA genes of streptomycetes and association of proteins to the tmRNA during development and cellular differentiation , 2008, Proteomics.

[23]  M. Pátek,et al.  Chromosomally encoded small antisense RNA in Corynebacterium glutamicum. , 2008, FEMS microbiology letters.

[24]  M. Pátek,et al.  Plasmid Vectors for Testing In Vivo Promoter Activities in Corynebacterium glutamicum and Rhodococcus erythropolis , 2007, Current Microbiology.

[25]  M. R̆ic̆icová,et al.  Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches. , 2007, Biochimica et biophysica acta.

[26]  R. Wilkinson,et al.  Enhanced Ex Vivo Stimulation of Mycobacterium tuberculosis-Specific T Cells in Human Immunodeficiency Virus-Infected Persons via Antigen Delivery by the Bordetella pertussis Adenylate Cyclase Vector , 2007, Clinical and Vaccine Immunology.

[27]  M. Pátek,et al.  Analysis of catRABC operon for catechol degradation from phenol-degrading Rhodococcus erythropolis , 2007, Applied Microbiology and Biotechnology.

[28]  R. Benz,et al.  Segments Crucial for Membrane Translocation and Pore-forming Activity of Bordetella Adenylate Cyclase Toxin* , 2007, Journal of Biological Chemistry.

[29]  L. Nováková,et al.  A eukaryotic-type serine/threonine protein kinase StkP of Streptococcus pneumoniae acts as a dimer in vivo. , 2007, Biochemical and biophysical research communications.

[30]  L. Nováková,et al.  Eukaryotic-Type Serine/Threonine Protein Kinase StkP Is a Global Regulator of Gene Expression in Streptococcus pneumoniae , 2007, Journal of bacteriology.

[31]  Jiri Vohradsky,et al.  A parallel genetic algorithm for single class pattern classification and its application for gene expression profiling in Streptomyces coelicolor , 2007, BMC Genomics.

[32]  J. Mašín,et al.  Third Activity of Bordetella Adenylate Cyclase (AC) Toxin-Hemolysin , 2007, Journal of Biological Chemistry.

[33]  G. Grossmann,et al.  Membrane potential governs lateral segregation of plasma membrane proteins and lipids in yeast , 2007, The EMBO journal.

[34]  Jiri Vohradsky,et al.  Nonlinear differential equation model for quantification of transcriptional regulation applied to microarray data of Saccharomyces cerevisiae , 2006, Nucleic acids research.

[35]  J. Vohradský,et al.  The iron‐regulated transcriptome and proteome of Neisseria meningitidis serogroup C , 2006, Proteomics.

[36]  P. Šebo,et al.  HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets. , 2006, FEMS immunology and medical microbiology.

[37]  C. Leclerc,et al.  High Frequency of CD4+ T Cells Specific for the TB10.4 Protein Correlates with Protection against Mycobacterium tuberculosis Infection , 2006, Infection and Immunity.

[38]  L. Nováková,et al.  Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae , 2006, Eukaryotic Cell.

[39]  S. Anderson,et al.  Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing. , 2006, American journal of respiratory and critical care medicine.

[40]  J. Bujnicki,et al.  Structural model for the multisubunit Type IC restriction–modification DNA methyltransferase M.EcoR124I in complex with DNA , 2006, Nucleic acids research.

[41]  J. Mašín,et al.  Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes , 2006, Infection and Immunity.

[42]  V. Heussler,et al.  Immunization with a Circumsporozoite Epitope Fused to Bordetella pertussis Adenylate Cyclase in Conjunction with Cytotoxic T-Lymphocyte-Associated Antigen 4 Blockade Confers Protection against Plasmodium berghei Liver-Stage Malaria , 2006, Infection and Immunity.

[43]  P. Branny,et al.  A Eukaryotic-Type Serine/Threonine Protein Kinase Is Required for Biofilm Formation, Genetic Competence, and Acid Resistance in Streptococcus mutans , 2006, Journal of bacteriology.

[44]  J. Vohradský,et al.  Systems level analysis of protein synthesis patterns associated with bacterial growth and metabolic transitions , 2006, Proteomics.

[45]  K. Sigler,et al.  Activity of yeast multidrug resistance pumps during growth is controlled by carbon source and the composition of growth-depleted medium: DiS-C3(3) fluorescence assay. , 2005, The international journal of biochemistry & cell biology.

[46]  R. Benz,et al.  Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells. , 2005, Biochemistry.

[47]  L. Váchová,et al.  Physiological regulation of yeast cell death in multicellular colonies is triggered by ammonia , 2005, The Journal of cell biology.

[48]  C. Chassagnole,et al.  Rational Design of a Corynebacterium glutamicum Pantothenate Production Strain and Its Characterization by Metabolic Flux Analysis and Genome-Wide Transcriptional Profiling , 2005, Applied and Environmental Microbiology.

[49]  K. Malínská,et al.  Differential effect of phosphatidylethanolamine depletion on raft proteins: further evidence for diversity of rafts in Saccharomyces cerevisiae. , 2005, Biochimica et biophysica acta.

[50]  R. Wilkinson,et al.  Efficient Ex Vivo Stimulation of Mycobacterium tuberculosis-Specific T Cells by Genetically Detoxified Bordetella pertussis Adenylate Cyclase Antigen Toxoids , 2005, Infection and Immunity.

[51]  L. Nováková,et al.  Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates , 2005, The FEBS journal.

[52]  I. Linhartova,et al.  The Neisseria meningitidis Outer Membrane Lipoprotein FrpD Binds the RTX Protein FrpC* , 2005, Journal of Biological Chemistry.

[53]  C. Buchrieser,et al.  Characterization of the flexible genome complement of the commensal Escherichia coli strain A0 34/86 (O83 : K24 : H31). , 2005, Microbiology.

[54]  M. Pátek,et al.  Feedback-Resistant Acetohydroxy Acid Synthase Increases Valine Production in Corynebacterium glutamicum , 2005, Applied and Environmental Microbiology.

[55]  K. Malínská,et al.  Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells , 2004, Journal of Cell Science.

[56]  J. Vohradský,et al.  Activation and expression of proteins during synchronous germination of aerial spores of Streptomyces granaticolor , 2004, Proteomics.

[57]  K. Sigler,et al.  Impact of the growth phase on the activity of multidrug resistance pumps and membrane potential of S. cerevisiae: effect of pump overproduction and carbon source. , 2004, Biochimica et biophysica acta.

[58]  C. Jacq,et al.  Sok2p Transcription Factor Is Involved in Adaptive Program Relevant for Long Term Survival of Saccharomyces cerevisiae Colonies*[boxs] , 2004, Journal of Biological Chemistry.

[59]  K. Firman,et al.  Cellular localization of Type I restriction-modification enzymes is family dependent. , 2004, Biochemical and biophysical research communications.

[60]  F. Goñi,et al.  Membrane Restructuring by Bordetella pertussis Adenylate Cyclase Toxin, a Member of the RTX Toxin Family , 2004, Journal of bacteriology.

[61]  V. Havlíček,et al.  A Novel “Clip-and-link” Activity of Repeat in Toxin (RTX) Proteins from Gram-negative Pathogens , 2004, Journal of Biological Chemistry.

[62]  I. Janatová,et al.  The absence of the Isw2p–Itc1p chromatin‐remodelling complex induces mating type‐specific and Flo11p‐independent invasive growth of Saccharomyces cerevisiae , 2004, Yeast.

[63]  S. Totevova,et al.  Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders , 2004, Journal of applied microbiology.

[64]  N. Alic,et al.  Lipid Hydroperoxides Activate the Mitogen-activated Protein Kinase Mpk1p in Saccharomyces cerevisiae* , 2003, Journal of Biological Chemistry.

[65]  D. Ladant,et al.  Interaction of Bordetella pertussis Adenylate Cyclase with CD11b/CD18 , 2003, Journal of Biological Chemistry.

[66]  M. Pátek,et al.  Function of Corynebacterium glutamicum promoters in Escherichia coli, Streptomyces lividans, and Bacillus subtilis. , 2003, Journal of biotechnology.

[67]  M. Pátek,et al.  Promoters of Corynebacterium glutamicum. , 2003, Journal of biotechnology.

[68]  K. Malínská,et al.  Visualization of protein compartmentation within the plasma membrane of living yeast cells. , 2003, Molecular biology of the cell.

[69]  R. Benz,et al.  Channel formation in model membranes by the adenylate cyclase toxin of Bordetella pertussis: effect of calcium. , 2003, Biochemistry.

[70]  H. Aguilaniu,et al.  The oncogenicRAS2val19 mutation locks respiration, independently of PKA, in a mode prone to generate ROS , 2003, The EMBO journal.

[71]  Jiri Vohradsky,et al.  Proteomic studies of diauxic lag in the differentiating prokaryote Streptomyces coelicolor reveal a regulatory network of stress‐induced proteins and central metabolic enzymes , 2003, Molecular microbiology.

[72]  M. Pátek,et al.  Control of rep Gene Expression in Plasmid pGA1 from Corynebacteriumglutamicum , 2003, Journal of bacteriology.

[73]  M. Pátek,et al.  Host-vector system for phenol-degrading Rhodococcus erythropolis based on Corynebacterium plasmids , 2003, Applied Microbiology and Biotechnology.

[74]  K. Sigler,et al.  Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H(+)-ATPase reconstituted into vesicles. , 2003, Biochimica et biophysica acta.

[75]  O. Benada,et al.  Expression of proteins and protein kinase activity during germination of aerial spores of Streptomyces granaticolor. , 2002, Biochemical and biophysical research communications.

[76]  W. Tanner,et al.  Phosphatidyl ethanolamine is essential for targeting the arginine transporter Can1p to the plasma membrane of yeast. , 2002, Biochimica et Biophysica Acta.

[77]  K. Sigler,et al.  Monitoring the kinetics and performance of yeast membrane ABC transporters by diS-C3(3) fluorescence. , 2002, The international journal of biochemistry & cell biology.

[78]  J. Loucká,et al.  Delivery of a MalE CD4+-T-Cell Epitope into the Major Histocompatibility Complex Class II Antigen Presentation Pathway by Bordetella pertussis Adenylate Cyclase , 2002, Infection and Immunity.

[79]  J. Bobek,et al.  Changes in ribosome function induced by protein kinase associated with ribosomes of Streptomyces collinus producing kirromycin. , 2001, Biochemical and biophysical research communications.

[80]  A. Hinnebusch,et al.  Dual Function of eIF3j/Hcr1p in Processing 20 S Pre-rRNA and Translation Initiation* , 2001, The Journal of Biological Chemistry.

[81]  M. Pátek,et al.  Identification of a novel gene involved in stable maintenance of plasmid pGA1 from Corynebacterium glutamicum. , 2001, Plasmid.

[82]  J. Vohradský Neural Model of the Genetic Network* , 2001, The Journal of Biological Chemistry.

[83]  R. Osicka,et al.  Neisseria meningitidis RTX Protein FrpC Induces High Levels of Serum Antibodies during Invasive Disease: Polymorphism of frpC Alleles and Purification of Recombinant FrpC , 2001, Infection and Immunity.

[84]  J. Vohradský,et al.  Genome resource utilization during prokaryotic development , 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[85]  K. Sigler,et al.  Fluorescence Behavior of the pH-Sensitive Probe Carboxy SNARF-1 in Suspension of Liposomes¶ , 2001, Photochemistry and photobiology.

[86]  J. Vohradský Neural network model of gene expression , 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[87]  S. Kohlwein,et al.  Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis , 2001, Molecular microbiology.

[88]  W. Tanner,et al.  Construction of phosphatidylethanolamine‐less strain of Saccharomyces cerevisiae. Effect on amino acid transport , 2001, Yeast.

[89]  V. Havlíček,et al.  Acylation of Lysine 983 Is Sufficient for Toxin Activity of Bordetella pertussis Adenylate Cyclase , 2001, The Journal of Biological Chemistry.

[90]  K. Sigler,et al.  Mechanisms of Saccharomyces Cerevisiae PMA1 H+-ATPase inactivation by Fe2+, H2O2 and Fenton reagents , 2001, Free radical research.

[91]  K. Firman,et al.  A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage of the subunit assembly pathway. , 2000, Journal of molecular biology.

[92]  J. Vohradský,et al.  Developmental Control of Stress Stimulons inStreptomyces coelicolor Revealed by Statistical Analyses of Global Gene Expression Patterns , 2000, Journal of bacteriology.

[93]  K. Firman,et al.  Localization of the type I restriction-modification enzyme EcoKI in the bacterial cell. , 2000, Biochemical and biophysical research communications.

[94]  S. Kohlwein,et al.  Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein. , 2000, Cell motility and the cytoskeleton.

[95]  O. Benada,et al.  Isolation and characterization of dcw cluster from Streptomyces collinus producing kirromycin. , 2000, Biochemical and biophysical research communications.

[96]  W. Tanner,et al.  Properties of a reconstituted eukaryotic hexose/proton symporter solubilized by structurally related non-ionic detergents: specific requirement of phosphatidylcholine for permease stability. , 2000, Biochimica et biophysica acta.

[97]  S. Kohlwein,et al.  Saccharomyces cerevisiae gene ISW2 encodes a microtubule‐interacting protein required for premeiotic DNA replication , 2000, Yeast.

[98]  C. Leclerc,et al.  Delivery of CD8+ T-Cell Epitopes into Major Histocompatibility Complex Class I Antigen Presentation Pathway by Bordetella pertussis Adenylate Cyclase: Delineation of Cell Invasive Structures and Permissive Insertion Sites , 2000, Infection and Immunity.

[99]  R. Benz,et al.  An amphipathic alpha-helix including glutamates 509 and 516 is crucial for membrane translocation of adenylate cyclase toxin and modulates formation and cation selectivity of its membrane channels. , 1999, The Journal of biological chemistry.

[100]  O. Benada,et al.  Expression of the Csp protein family upon cold shock and production of tetracycline in Streptomyces aureofaciens. , 1999, Biochemical and biophysical research communications.

[101]  C. Leclerc,et al.  In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying multiple copies of a viral CD8(+) T-cell epitope. , 1999, FEMS immunology and medical microbiology.

[102]  M. Pátek,et al.  Analysis of the Corynebacterium glutamicum dapA Promoter , 1999, Journal of bacteriology.

[103]  K. Demnerova,et al.  Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl‐contaminated soil and sediment in the Czech Republic , 1999, Journal of applied microbiology.

[104]  K. Sigler,et al.  Factors and processes involved in membrane potential build-up in yeast: diS-C3(3) assay. , 1999, The international journal of biochemistry & cell biology.

[105]  V. Havlíček,et al.  The Conserved Lysine 860 in the Additional Fatty-acylation Site of Bordetella pertussis Adenylate Cyclase Is Crucial for Toxin Function Independently of Its Acylation Status* , 1999, The Journal of Biological Chemistry.

[106]  L. Váchová,et al.  In Vivo and In Vitro Function of the Intracellular Proteolytic Apparatus in Nongrowing Bacillus megaterium Under Heat Stress , 1999, Current Microbiology.

[107]  P. Branny,et al.  Pkg2, a Novel Transmembrane Protein Ser/Thr Kinase of Streptomyces granaticolor , 1999, Journal of bacteriology.

[108]  M. Pátek,et al.  An integron of class 1 is present on the plasmid pCG4 from gram-positive bacterium Corynebacterium glutamicum. , 1998, FEMS microbiology letters.

[109]  P. Branny,et al.  Characterisation of two putative protein Ser/Thr kinases from actinomycete Streptomyces granaticolor both endowed with different properties. , 1998, European journal of biochemistry.

[110]  M. Pátek,et al.  Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum , 1998 .

[111]  K. Mikulík,et al.  Characterization offtsZGene and Its Protein Product fromStreptomyces collinusProducing Kirromycin , 1998 .

[112]  L. Valášek,et al.  Rpg1, the Saccharomyces cerevisiae Homologue of the Largest Subunit of Mammalian Translation Initiation Factor 3, Is Required for Translational Activity* , 1998, The Journal of Biological Chemistry.

[113]  M. Pátek,et al.  Analysis of the leuB gene from Corynebacterium glutamicum , 1998, Applied Microbiology and Biotechnology.

[114]  K. Firman,et al.  Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I. , 1998, Biological chemistry.

[115]  L. Valášek,et al.  RPG1: an essential gene of Saccharomyces cerevisiae encoding a 110-kDa protein required for passage through the G1 phase , 1998, Current Genetics.

[116]  T. Caspari,et al.  Post‐translational fate of CAN1 permease of Saccharomyces cerevisiae , 1998, Yeast.

[117]  K. Mikulík,et al.  Protein kinase associated with ribosomes phosphorylates ribosomal proteins of Streptomyces collinus. , 1997, Biochemical and biophysical research communications.

[118]  M. Breitenbach,et al.  Mutants in the Saccharomyces cerevisiae RAS2 gene influence life span, cytoskeleton, and regulation of mitosis. , 1997, Canadian journal of microbiology.

[119]  M. Opekarová,et al.  On the unidirectionality of arginine uptake in the yeast Saccharomyces cerevisiae. , 1997, FEMS microbiology letters.

[120]  J. Vohradský,et al.  Plasmid pGA1 from Corynebacterium glutamicum codes for a gene product that positively influences plasmid copy number , 1997, Journal of bacteriology.

[121]  M. Pátek,et al.  Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif. , 1996, Microbiology.

[122]  K. Sigler,et al.  Cd2+-induced damage to yeast plasma membrane and its alleviation by Zn2+: studies on Schizosaccharomyces pombe cells and reconstituted plasma membrane vesicles , 1996, Archives of Microbiology.

[123]  Z. Dobrova,et al.  Improved method for rapid purification of protein kinase from streptomycetes. , 1996, Journal of biochemical and biophysical methods.

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[127]  W. Tanner,et al.  Nystatin changes the properties of transporters for arginine and sugars An in vitro study , 1994, FEBS letters.

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[129]  L. Váchová,et al.  Heat and osmotic stress enhance the development of cytoplasmic serine proteinase activity in sporulating Bacillus megaterium. , 1994, Biochemistry and molecular biology international.

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