Characteristics of murine C‐type viruses. III. Antigenic conversion and focus induction

Viruses produced by two Moloney lymphoma sublines (Y and Yr), and their hybrids with a mouse L cell subline (YL and YrL) were tested for antigenic conversion and focus induction on JLS‐V9 and D56 (S + L −) cells. Two infectivity patterns were encountered. Both indicator cell lines were converted antigenically by the Y and YT viruses, whereas only D56 cells, and not JLS‐V9 cells, were converted by the Yr and YrL viruses. When infection of D56 cells was scored by both antigenic conversion and focus induction, further variations in infectivity patterns were found. The viruses produced by the YAC‐A9 and YACIR‐A9 hybrid cells (YL and YrL) appeared to be similar to the viruses produced by each of the lymphoma parents.

[1]  E. Fenyö,et al.  Virus-associated surface antigens on L cells and Moloney lymphoma cells. , 1974, Journal of the National Cancer Institute.

[2]  E. Fenyö,et al.  Characteristics of murine C‐type viruses. I. Independent assortment of infectivity in one in vivo and four in vitro assays , 1973, International journal of cancer.

[3]  R. Gilden,et al.  Variations in sarcoma and leukemia virus activity in somatic cell hybrids , 1972, International journal of cancer.

[4]  E. Fenyö,et al.  Murine Leukemia Virus Assay Techniques: A Comparative Study , 1972, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[5]  G. Klein,et al.  Surface antigens and release of virus in hybrid cells produced by the fusion of A9 fibroblasts with Moloney Lymphoma cells. , 1971, Experimental cell research.

[6]  R. Bassin,et al.  Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[7]  E. Fenyö,et al.  MEMBRANE IMMUNOFLUORESCENCE ON CULTURED INDICATOR CELLS AS A MEASURE OF VIRUS PRODUCTION BY MOUSE L CELLS AND BY TWO MOLONEY LYMPHOMA SUBLINES DIFFERING IN IMMUNOSENSITIVITY * , 1971, Annals of the New York Academy of Sciences.

[8]  R. Bassin,et al.  Rapid Cell Culture Assay Technique for Murine Leukaemia Viruses , 1971, Nature.

[9]  G. Klein,et al.  Isoantigen expression in hybrid mouse cells. , 1970, Experimental cell research.

[10]  R. Bassin,et al.  Isolation of murine sarcoma virus‐transormed mouse cells which are negative for leukemia virus from agar suspension cultures , 1970 .

[11]  E. Fenyö,et al.  Tissue culture assay for Moloney leukemia virus. , 1970, Journal of the National Cancer Institute.

[12]  E. Fenyö,et al.  Studies on the relations between virus release and cellular immunosensitivity in Moloney lymphomas. , 1969, Journal of the National Cancer Institute.

[13]  J. Littlefield.  Three Degrees of Guanylic Acid — Inosinic Acid Pyrophosphorylase Deficiency in Mouse Fibroblasts , 1964, Nature.

[14]  W. Szybalski,et al.  Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait. , 1962, Proceedings of the National Academy of Sciences of the United States of America.

[15]  L. Siminovitch,et al.  An air-drying technique for flattening chromosomes in mammalian oells grown in vitro. , 1958, Stain technology.

[16]  G. Klein,et al.  Selection of an immunoresistant Moloney lymphoma subline with decreased concentration of tumor-specific surface antigens. , 1968, Journal of the National Cancer Institute.