Potassium‐ and Carbachol‐Evoked Release of [3H]Noradrenaline from Human Neuroblastoma Cells, SH‐SY5Y

Abstract: The human neuroblastoma clone SH‐SY5Y expresses potassium‐, carbachol‐, and calcium ionophore A23187‐evoked, calcium‐dependent release of [3H]noradrenaline. Release in response to carbachol and potassium was greater than additive. Atropine (Ki= 0.33 nM), hexahydrosiladifenidol (Ki= 18 nM), and pirenzepine (Ki= 1,183 nM) completely inhibited the carbachol‐evoked noradrenaline release, an order of potency suggesting that an M3 receptor was linked to release. In contrast, noradrenaline release was only partially inhibited by the M2‐selective antagonists meth‐octramine (10‐4M) and AFDX‐116 (10‐4M), by ∼14 and 46%, respectively. The nicotinic antagonist d‐tubocurarine (10‐4M) resulted in a partial inhibition of release, a finding suggesting that a nicotinic receptor may also be involved. SH‐SY5Y provides a suitable cell line in which to study the biochemical mechanisms underlying the cholinergic receptor regulation of noradrenaline release.

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