Surface Enhanced Raman Spectroscopy (SERS) of Fluid Supported Lipid Bilayers.

Supported lipid bilayers are essential model systems for studying biological membranes and for membrane-based sensor development. Surface enhanced Raman spectroscopy (SERS) stands to add considerably to our understanding of the dynamics and interactions of these systems through the direct chemical information. Despite this potential, SERS of lipid bilayers is not routinely achieved. Here, we carried out the first measurements of a solid supported lipid bilayer on a SERS-active substrate, and characterized the bilayer using SERS, atomic force microscopy (AFM), surface plasmon resonance (SPR) spectroscopy, ellipsometry, and fluorescence recovery after photobleaching (FRAP). The creation of a fluid, SERS-active supported lipid bilayer was accomplished through use of a novel silica-coated silver film-over-nanosphere (AgFON) substrate. These substrates offer a powerful new platform to couple common surface techniques that are challenging on the nanoscale, e.g. ellipsometry and FRAP, with SERS for studying biological membranes and their dynamics.

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