3D Fluorescent Spots Detection in Line-Scanning Confocal Microscopy

This paper describes a method to detect fluorescent PSF-like spots in 3D line-scanning confocal microscopy. We suppose that fluorescent spots are small enough to be characterized by the Point Spread function (PSF) of the microscope. To localize spots, we use a derivative expression of the image previously convolved by a Gaussian approximating the PSF. The algorithm validation is made on 3D synthetic images with fluorescent background structures and on a 3D fluorescent biological image.