Methods to find aponeurosis and tendon stiffness and the onset of muscle contraction

A method to measure small movements of living tissues either large or small is presented. The method is based on the detection of changes in reflected infrared light. An optocoupler (coupled photodiode and photodetector) and a small (< 1 cm2) mirror were used. The optocoupler (OC) has a low cost and it can be calibrated easily. It can be also used as the transducer of a strain-gage. Three different uses are shown: (a) as a strain-gage transducer; (b) detection of tendon and aponeurosis movements in large muscles (cat soleus); (c) detection of the onset of muscle contraction. Movements of less than 1 microm can be detected with the aid of automatic averaging of the signals. Concerning the second use (b), it permits the estimation of tendon stretch. Concerning the third use, the onset of muscle movement precedes by at least 2 ms that of the force recorded at the tendon.

[1]  J. J. Alvarado-Gil,et al.  A new optical method based on laser 'knife edge' detection for monitoring an early embryonic beating heart , 1995 .

[2]  A. Hill The abrupt transition from rest to activity in muscle , 1949, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[3]  G. Loeb,et al.  Mechanical properties of aponeurosis and tendon of the cat soleus muscle during whole‐muscle isometric contractions , 1995, Journal of morphology.

[4]  Archibald Vivian Hill,et al.  The series elastic componet of muscle , 1950, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[5]  P. Matthews,et al.  Mammalian muscle receptors and their central actions , 1974 .

[6]  J. A. Hoffer,et al.  New transducers for measuring muscle length in unrestrained animals , 1992, Journal of Neuroscience Methods.

[7]  J. Spudich,et al.  Single myosin molecule mechanics: piconewton forces and nanometre steps , 1994, Nature.

[8]  S. W. Kuffler,et al.  Stretch receptor discharges during muscle contraction , 1951, The Journal of physiology.

[9]  P. Rack,et al.  Elastic properties of the cat soleus tendon and their functional importance. , 1984, The Journal of physiology.

[10]  R. Griffiths Ultrasound transit time gives direct measurement of muscle fibre length in vivo , 1987, Journal of Neuroscience Methods.

[11]  R. Griffiths,et al.  Roles of muscle activity and load on the relationship between muscle spindle length and whole muscle length in the freely walking cat. , 1989, Progress in brain research.

[12]  R. Reinking,et al.  "Sensory partitioning" of cat medial gastrocnemius muscle by its muscle spindles and tendon organs. , 1981, Journal of neurophysiology.

[13]  R. Griffiths Shortening of muscle fibres during stretch of the active cat medial gastrocnemius muscle: the role of tendon compliance. , 1991, The Journal of physiology.