Secondary sites for integration mediated by the Tn21 integrase

The integrase encoded by the integron of the transposon Tn21 can mediate the site‐specific fusion of two plasmids if there is a recombination hot spot (59 bp element) in one of them and the sequence GWTMW in the other. The use of this latter, loosely defined site explains how antibiotic‐resistance genes could first become associated with integrons.

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