Inhibitory effects of butyrate on biological hydrogen production with mixed anaerobic cultures.

In this study batch experiments were conducted to investigate the inhibitory effects of butyrate addition on hydrogen production from glucose by using anaerobic mixed cultures. Experimental results showed that addition of butyrate at 4.18 and 6.27 g/l only slightly inhibited hydrogen production, and addition of butyrate at 8.36-12.54 g/l imposed a moderate inhibitory effect on hydrogen production. At addition of 25.08 g/l, butyrate had a strong inhibitory influence on substrate degradation and hydrogen production. The distribution of the volatile fatty acids produced from the acidogeneisis of glucose was significantly influenced by the addition of butyrate. The inhibition of butyrate addition on hydrogen production was described well by a non-competitive and non-linear inhibition model, with the maximum hydrogen production rate of 59.3 ml/g-SS/h, critical added butyrate concentration of 25.08 g/l, and inhibition degree of 0.323, respectively. The C(I,50) values (the butyrate concentration at which bioactivity is reduced by 50%) for hydrogen production rate and yield were estimated as 19.39 and 20.78 g/l of added butyrate, respectively.

[1]  Tatsuya Noike,et al.  Effect of pH on the microbial hydrogen fermentation , 2002 .

[2]  Inhibition on acidogenesis of dairy wastewater by zinc and copper. , 2001, Environmental technology.

[3]  O. Levenspiel,et al.  Extended monod kinetics for substrate, product, and cell inhibition , 1988, Biotechnology and bioengineering.

[4]  J. Lay,et al.  Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. , 2001, Biotechnology and bioengineering.

[5]  Hanqing Yu,et al.  Roles of pH in biologic production of hydrogen and volatile fatty acids from glucose by enriched anaerobic cultures , 2004, Applied biochemistry and biotechnology.

[6]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[7]  A. Stams,et al.  Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus. , 2003, Biotechnology and bioengineering.

[8]  J. Miyake,et al.  Photoproduction of hydrogen from glucose by a co-culture of a photosynthetic bacterium and Clostridium butyricum , 1984 .

[9]  T. Noike,et al.  Biological hydrogen potential of materials characteristic of the organic fraction of municipal solid wastes. , 2000, Water science and technology : a journal of the International Association on Water Pollution Research.

[10]  A.J.C.M. Matthijsen,et al.  Product inhibition in the acid forming stage of the anaerobic digestion process , 1982 .

[11]  F. Smith,et al.  Colorimetric Method for Determination of Sugars and Related Substances , 1956 .

[12]  C. Stewart.  Some Effects of Phosphate and Volatile Fatty Acid Salts on the Growth of Rumen Bacteria , 1975 .

[13]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[14]  Chiu-Yue Lin,et al.  Using sucrose as a substrate in an anaerobic hydrogen-producing reactor , 2003 .

[15]  T. Noike,et al.  Hydrogen fermentation of organic municipal wastes , 2000 .

[16]  P. Mccarty,et al.  Bioassay for monitoring biochemical methane potential and anaerobic toxicity , 1979 .

[17]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .