Fluorescent detection of cholesterol using β-cyclodextrin functionalized graphene.

A fluorescence based cholesterol detection method has been developed using competitive host-guest interaction between graphene bound β-cyclodextrin (β-CD) with rhodamine 6G (R6G) and cholesterol. Fluorescence of β-CD incorporated R6G is quenched by graphene but is 'turned on' by cholesterol as it replaces R6G from the β-CD host.

[1]  S. Stankovich,et al.  Stable aqueous dispersions of graphitic nanoplatelets via the reduction of exfoliated graphite oxide in the presence of poly(sodium 4-styrenesulfonate) , 2006 .

[2]  E. Wang,et al.  Cyclodextrin functionalized graphene nanosheets with high supramolecular recognition capability: synthesis and host-guest inclusion for enhanced electrochemical performance. , 2010, ACS nano.

[3]  Igor L. Medintz,et al.  A Reagentless Biosensing Assembly Based on Quantum Dot–Donor Förster Resonance Energy Transfer , 2005 .

[4]  M. Jauhiainen,et al.  Human plasma lecithin-cholesterol acyltransferase. An elucidation of the catalytic mechanism. , 1986, The Journal of biological chemistry.

[5]  N. Myant.  The Biology of Cholesterol and Related Steroids , 2014 .

[6]  W. J. Johnson,et al.  Cellular Cholesterol Efflux Mediated by Cyclodextrins , 1996, The Journal of Biological Chemistry.

[7]  H. Kwak,et al.  Removal of Cholesterol from Cheddar Cheese by β-Cyclodextrin , 2002 .

[8]  Chunhai Fan,et al.  A graphene-enhanced molecular beacon for homogeneous DNA detection. , 2010, Nanoscale.

[9]  Chunhai Fan,et al.  A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis , 2010 .

[10]  P. Kamat,et al.  Fortification of CdSe quantum dots with graphene oxide. Excited state interactions and light energy conversion. , 2012, Journal of the American Chemical Society.

[11]  C. Rao,et al.  Quenching of fluorescence of aromatic molecules by graphene due to electron transfer , 2010, 1009.3700.

[12]  C. R. Raj,et al.  Development of an Amperometric Cholesterol Biosensor Based on Graphene-Pt Nanoparticle Hybrid Material , 2010 .

[13]  R. Breslow,et al.  CHOLESTEROL RECOGNITION AND BINDING BY CYCLODEXTRIN DIMERS , 1996 .

[14]  N. Jana,et al.  Peptide-functionalized colloidal graphene via interdigited bilayer coating and fluorescence turn-on detection of enzyme. , 2011, ACS applied materials & interfaces.

[15]  R. Ruoff,et al.  All-organic vapor sensor using inkjet-printed reduced graphene oxide. , 2010, Angewandte Chemie.

[16]  L R Faulkner,et al.  Determination of cholesterol and cholesterol ester with novel enzyme microsensors. , 1993, Analytical chemistry.

[17]  Heng Zhang,et al.  Tailoring of biomimetic high-density lipoprotein nanostructures changes cholesterol binding and efflux. , 2012, ACS nano.

[18]  G. Eda,et al.  Graphene oxide as a chemically tunable platform for optical applications. , 2010, Nature chemistry.

[19]  Jian-hui Jiang,et al.  Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection. , 2010, Analytical chemistry.

[20]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[21]  Huang-Hao Yang,et al.  A graphene platform for sensing biomolecules. , 2009, Angewandte Chemie.

[22]  R. Ruoff,et al.  The chemistry of graphene oxide. , 2010, Chemical Society reviews.

[23]  Y. Inoue,et al.  Complexation Thermodynamics of Cyclodextrins. , 1998, Chemical reviews.

[24]  K. Nakajima,et al.  Highly sensitive cholesterol assay with enzymatic cycling applied to measurement of remnant lipoprotein-cholesterol in serum. , 2002, Clinical chemistry.

[25]  Zhihong Liu,et al.  Biosensing platform based on fluorescence resonance energy transfer from upconverting nanocrystals to graphene oxide. , 2011, Angewandte Chemie.

[26]  F. Maxfield,et al.  Endocytosis of beta-cyclodextrins is responsible for cholesterol reduction in Niemann-Pick type C mutant cells , 2010, Proceedings of the National Academy of Sciences.

[27]  E. Bekyarova,et al.  Diels-Alder chemistry of graphite and graphene: graphene as diene and dienophile. , 2011, Journal of the American Chemical Society.

[28]  P. Linsel-Nitschke,et al.  HDL as a target in the treatment of atherosclerotic cardiovascular disease , 2005, Nature Reviews Drug Discovery.

[29]  Anando Devadoss,et al.  Detection of Cholesterol through Electron Transfer to Cholesterol Oxidase in Electrode-Supported Lipid Bilayer Membranes , 2002 .

[30]  Hua Xiong,et al.  Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels , 2011 .

[31]  D. M. Amundson,et al.  Fluorometric method for the enzymatic determination of cholesterol. , 1999, Journal of biochemical and biophysical methods.

[32]  Elina Ikonen,et al.  Cellular cholesterol trafficking and compartmentalization , 2008, Nature Reviews Molecular Cell Biology.

[33]  Cesar A. López,et al.  Molecular Mechanism of Cyclodextrin Mediated Cholesterol Extraction , 2011, PLoS Comput. Biol..

[34]  David C. Zhu,et al.  Synthesis of β-cyclodextrin conjugated superparamagnetic iron oxide nanoparticles for selective binding and detection of cholesterol crystals. , 2012, Chemical communications.

[35]  Bo Tang,et al.  A novel assembly of Au NPs-beta-CDs-FL for the fluorescent probing of cholesterol and its application in blood serum. , 2008, The Analyst.

[36]  M. M. Lucchese,et al.  Quantifying ion-induced defects and Raman relaxation length in graphene , 2010 .

[37]  Andrew G. Glen,et al.  APPL , 2001 .