Influence of egg lipids and fatty acids on egg viability, and their utilization during embryonic development of spotted wolf‐fish, Anarhichas minor Olafsen

The changes in egg lipids and fatty acid compositions that occur during embryonic development of spotted wolf-fish, Anarhichas minor, were examined by monitoring individual egg batches from the time of spawning (egg stripping) until hatching. The lipids, present as 3.7±0.1% of the wet mass of the freshly stripped eggs, contained high percentages of monoenes (monounsaturated fatty acids (MUFAs), ca. 33%) and polyenes (ca. 43%) and approximately 20% saturated fatty acids (SFAs). The fatty acid profiles were dominated by a small number of fatty acids. The major SFA was 16:0 (ca. 14%), the dominant MUFA was 18:1 n-9 (ca. 21%), and among the polyenes, the n-3 highly unsaturated fatty acids (HUFAs) 22:6 n-3 docosahexaenoic acid (DHA) and 20:5 n-3 eicosapentaenoic acid (EPA) were present in the highest concentrations (EPA, ca. 16%; DHA, ca. 19%). The n-6 HUFA 20:4 n-6 arachidonic acid (AA) was present as ca. 1% of the total fatty acids in the freshly stripped eggs. This resulted in an AA:EPA of ca. 0.07, which is lower than reported for eggs of many other fish species. As embryonic development progressed, the percentage contribution of AA to the total fatty acids almost doubled. There were also increases in the relative proportions of SFAs (due mainly to an increase in the percentage of 16:0 to ca. 16% at hatch) and DHA (to ca. 23%), and there was a corresponding decrease in the percentage of MUFAs (mostly brought about by a decrease in the percentage of 18:1 n-9 to ca. 18% at hatch). The most marked changes occurred towards the end of incubation. The percentage of EPA changed little during incubation. This implies that there was selective retention of DHA, 16:0 and AA, and these fatty acids were probably incorporated into cell membranes. MUFAs, particularly 18:1 n-9, seem to have been catabolized to provide energy for the developing embryo, and some EPA also seems to have been utilized as an energy source. Survival of eggs to the eyed stage (range ca. 10–80%) and to hatch (ca. 5–75%) was negatively correlated with the %AA, %EPA and AA:DHA of the freshly stripped eggs. There was also a negative correlation between AA:EPA and egg survival, which implies that there is not a universal requirement for a high AA:EPA to ensure high rates of survival of fish eggs.

[1]  J. G. Bell,et al.  Lipids and lipid antioxidant systems in developing eggs of salmon (Salmo salar) , 1985, Lipids.

[2]  D. Tocher,et al.  Fatty acid composition of phospholipids and neutral lipids during embryonic and early larval development in Atlantic herring (Clupea harengus, L.) , 1985, Lipids.

[3]  D. Tocher,et al.  Analyses of lipids and fatty acids in ripe roes of some Northwest European marine fish , 1984, Lipids.

[4]  K. Taberski,et al.  Lipid and fatty acid composition of the endogenous energy sources of striped bass (Morone saxatilis) eggs , 1983, Lipids.

[5]  M. D. Wiegand.  Utilization of yolk fatty acids by goldfish embryos and larvae , 1996, Fish Physiology and Biochemistry.

[6]  M. D. Wiegand.  Composition, accumulation and utilization of yolk lipids in teleost fish , 1996, Reviews in Fish Biology and Fisheries.

[7]  E. Moksness,et al.  Reproductive biology, early ontogeny, and effect of temperature on development in wolffish: comparison with salmon , 1994, Aquaculture International.

[8]  T. Takeuchi,et al.  Effect of arachidonic acid levels in broodstock diet on larval and egg quality of Japanese flounder Paralichthys olivaceus. , 2003 .

[9]  J. G. Bell,et al.  Arachidonic acid in aquaculture feeds: current status and future opportunities , 2003 .

[10]  S. Mayne,et al.  Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. , 2003, The Journal of nutrition.

[11]  T. Yamamoto,et al.  Effects of high levels of n-3 HUFA in broodstock diet on egg quality and egg fatty acid composition of Japanese flounder, Paralichthys olivaceus , 2002 .

[12]  P. Blier,et al.  Biological and technical evaluation of the potential of marine and anadromous fish species for cold-water mariculture , 2002 .

[13]  A. Tacon,et al.  Effect of broodstock nutrition on reproductive performance of fish , 2001 .

[14]  H. Johnsen,et al.  Holding temperature during the breeding season influences final maturation and egg quality in common wolffish , 2001 .

[15]  G. Dunstan,et al.  Differences in egg quality between wild striped trumpeter (Latris lineata) and captive striped trumpeter that were fed different diets. , 2001 .

[16]  N. Bromage,et al.  Development of broodstock diets for the European Sea Bass (Dicentrarchus labrax) with special emphasis on the importance of n−3 and n−6 highly unsaturated fatty acid to reproductive performance , 1999 .

[17]  E. Almansa,et al.  Influence of broodstock gilthead seabream (Sparus aurata L.) dietary fatty acids on egg quality and egg fatty acid composition throughout the spawning season , 1999 .

[18]  J. Pickova,et al.  Comparison of fatty acid composition and astaxanthin content in healthy and by M74 affected salmon eggs from three Swedish river stocks , 1998 .

[19]  J. G. Bell,et al.  The impact of seasonal alteration in the lipid composition of broodstock diets on egg quality in the European sea bass , 1997 .

[20]  J. Pickova,et al.  Early embryonic cleavage pattern, hatching success, and egg-lipid fatty acid composition: comparison between two cod (Gadus morhua) stocks , 1997 .

[21]  J. G. Bell,et al.  Effects of broodstock dietary lipid on fatty acid compositions of eggs from sea bass (Dicentrarchus labrax) , 1997 .

[22]  I. Benzie.  Lipid peroxidation: a review of causes, consequences, measurement and dietary influences. , 1996, International journal of food sciences and nutrition.

[23]  C. Haux,et al.  Fatty-acid composition of ovulated eggs from wild and cultured turbot (Scophthalmus maximus) in relation to yolk and oil globule lipids , 1996 .

[24]  John R. Vercellotti,et al.  Lipid oxidation in foods , 1993 .

[25]  R. Henderson.  Fatty acid metabolism in freshwater fish with particular reference to polyunsaturated fatty acids. , 1996, Archiv fur Tierernahrung.

[26]  R. Finn,et al.  Physiological energetics of developing embryos and yolk-sac larvae of Atlantic cod (Gadus morhua). II. Lipid metabolism and enthalpy balance , 1995 .

[27]  M. Salhi,et al.  Effect of n − 3 HUFA level in broodstock diets on egg quality of gilthead sea bream (Sparus aurata L.) , 1995 .

[28]  R. Jacob.  The integrated antioxidant system , 1995 .

[29]  M. Jobling,et al.  Effect of temperature on reproductive development in Arctic charr, Salvelinus alpinus (L.) , 1995 .

[30]  G. Kissil,et al.  The kinetics of nutrient incorporation into body tissues of gilthead seabream (Sparus aurata) females and the subsequent effects on egg composition and egg quality , 1994, British Journal of Nutrition.

[31]  G. Mourente,et al.  Biochemical composition and fatty acid content of fertilized eggs, yolk sac stage larvae and first-feeding larvae of the Senegal sole (Solea senegalensis Kaup) , 1994 .

[32]  A. C. Chan.  Partners in defense, vitamin E and vitamin C. , 1993, Canadian journal of physiology and pharmacology.

[33]  J. Mcevoy,et al.  Effect of spawning fast on lipid and fatty acid composition of eggs of captive turbot (Scophthalmus maximus L.) , 1993 .

[34]  H. Ashton,et al.  Fatty Acid Composition of Lipids in the Eggs and Alevins from Wild and Cultured Chinook Salmon (Oncorhynchus tshawytscha) , 1993 .

[35]  A. Anderson,et al.  Lipid classes and fatty acid composition of the eggs of some Australian fish , 1990 .

[36]  S. Falk‐Petersen,et al.  Lipids in atlantic halibut (Hippoglossus hippoglossus) eggs from planktonic samples in Northern Norway , 1989 .

[37]  J. Sargent,et al.  Changes in lipid content, lipid class composition and fatty acid composition of developing eggs and unfed larvae of cod (Gadus morhua) , 1988 .

[38]  O. Grahl-Nielsen,et al.  Fatty Acid Composition in Eggs of Atlantic Cod (Gadus morhua) , 1988 .

[39]  R. Olsen,et al.  Initial feeding of wolf fish (Anarhichas lupus L.) fry , 1987 .

[40]  D. Tocher,et al.  The lipid composition and biochemistry of freshwater fish. , 1987, Progress in lipid research.

[41]  A. Cossins,et al.  The Adaptation of Membrane Structure and Lipid Composition to Cold , 1985 .

[42]  R. Linko,et al.  Fatty acids in the roe lipids of common food fishes. , 1984, Comparative biochemistry and physiology. B, Comparative biochemistry.

[43]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.