Quantitative Analyses of Osteogenic- differentiation-related Gene Expressions in Human Osteoblast-like Cells (SaOS-2) Cultured on Hydroxyapatite and Titanium

The purpose of this study was to examine osteogenic differentiation behaviors at gene expression levels of osteoblast-like cells (SaOS-2) when cultured on polystyrene (PS), hydoroxyapatite (HAP) and titanium (Ti) for 8, 16 and 24 days, using six primer sets such as those for genes (mRNAs) of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alkaline phosphatase (ALPL), type 1 collagen (COL1A1), osteopontin (SPP1), bone Sialo protein (IBSP) and osteocalcin pre-cursor (BGLAP). With respect to GAPDH control, ALPL and COL1A1; SPP1 and IBSP; and BGLAP were used as initial-stage, middle-to-late-stage, and last-stage osteogenic differentiation phenotype markers, respectively. Quantitative real-time RT-PCR analyses revealed that HAP most differentiated SaOS-2, followed by Ti while PS did least. It was re-confirmed that with respect to PS, HAP was an ex-cellent osteo-conductive material while Ti also exerted moderate osteo-conductive effects on SaOS-2 in the cell culture condition.

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