Flow cytometric analysis of DNA content and keratins by using CK7, CK8, CK18, CK19, and KL1 monoclonal antibodies in benign and malignant human breast tumors.

We have used a double-labelling flow cytometry analysis of keratin (CK) and DNA in breast cancer. Five monoclonal anti-keratin antibodies were tested: KL1 recognizing Mr 55,000-57,000 keratins, and "anti-glandular epithelia," LE41, RGE-53, and LP2K specific for CK n. 7, 8, 18, and 19 of Moll's classification, respectively. Flow cytometric (DNA-CK) analysis was performed on 10 benign and 19 malignant human breast tumors. All the benign tumors were diploid and 63% of the malignant tumors were aneuploid. This technique permits the analysis of DNA in the epithelial fraction alone. In aneuploid tumors, gating the DNA-keratin-positive population allowed accurate DNA analysis without interference due to debris background and non-epithelial cells. Moreover, double-labelling using the CK19 antibody gave a better identification of near-diploid tumors. An enhancement of keratin expression in malignant tumors was observed with CK 19 (P less than 0.001), KL1 (P less than 0.01), CK 8 (P less than 0.05), and CK18 (n.s.) compared to benign tumors. The comparison of keratin expression in aneuploid and diploid malignant tumors revealed reduced CK8, CK18, and CK19 in the former.

[1]  F. Spyratos,et al.  Prognostic value of estrogen and progesterone receptors in primary infiltrating ductal breast cancer. A sequential multivariate analysis of 1262 patients. , 1989, European journal of cancer & clinical oncology.

[2]  I. Leigh,et al.  Keratin 19: predicted amino acid sequence and broad tissue distribution suggest it evolved from keratinocyte keratins. , 1989, The Journal of investigative dermatology.

[3]  M. Tubiana-Hulin,et al.  Sequential cytopunctures during preoperative chemotherapy for primary breast carcinoma. Cytomorphologic changes, initial tumor ploidy, and tumor regression , 1989, Cancer.

[4]  F. Spyratos,et al.  Cytokeratin analysis of breast and leukemia tumor cell lines by flow cytometry , 1989, Biology of the cell.

[5]  W. McGuire,et al.  DNA flow cytometry and prognostic factors in 1331 frozen breast cancer specimens , 1988, Cancer.

[6]  F. Spyratos,et al.  Flow cytometric study of DNA distribution in cytopunctures of benign and malignant breast lesions. , 1987, Analytical and quantitative cytology and histology.

[7]  E. Fuchs,et al.  Regulation of human mesothelial cell differentiation: opposing roles of retinoids and epidermal growth factor in the expression of intermediate filament proteins , 1987, The Journal of cell biology.

[8]  O. Kallioniemi,et al.  Tumour DNA ploidy as an independent prognostic factor in breast cancer. , 1987, British Journal of Cancer.

[9]  W. McGuire,et al.  Evaluation of a modeling system for S-phase estimation in breast cancer by flow cytometry. , 1987, Cancer research.

[10]  R. Gelber,et al.  Association of DNA index and S-phase fraction with prognosis of nodes positive early breast cancer. , 1987, Cancer research.

[11]  M. Kaufmann,et al.  Influence of systematic errors on the evaluation of the S phase portions from DNA distributions of solid tumors as shown for 328 breast carcinomas. , 1987, Cytometry.

[12]  E. Fuchs,et al.  Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization , 1987, The Journal of cell biology.

[13]  M. Gerber,et al.  Comparison of cytokeratin expression in primary and metastatic carcinomas. Diagnostic application in surgical pathology. , 1987, American journal of clinical pathology.

[14]  R. W. Baldwin,et al.  Tumour aneuploidy, prognostic parameters and survival in primary breast cancer. , 1987, British Journal of Cancer.

[15]  F. Debruyne,et al.  Tissue‐specific markers in flow cytometry of urological cancers. III. Comparing chromosomal and flow cytometric dna analysis of bladder tumors , 1987, International journal of cancer.

[16]  R. Moll.  Diversity of cytokeratins in carcinomas. , 1987, Acta histochemica. Supplementband.

[17]  F. Debruyne,et al.  Application of antibodies to intermediate filament proteins as tissue-specific probes in the flow cytometric analysis of complex tumors. , 1986, Analytical and quantitative cytology and histology.

[18]  P. van den Broek,et al.  Cell size, DNA, and cytokeratin analysis of human head and neck tumors by flow cytometry. , 1986, Cytometry.

[19]  K. Weber,et al.  Keratin polypeptide distribution in benign and malignant breast tumors: subdivision of ductal carcinomas using monoclonal antibodies , 1986, Virchows Archiv. B, Cell pathology including molecular pathology.

[20]  E. Lane,et al.  Keratin Antigens in Differentiating Skin , 1985, Annals of the New York Academy of Sciences.

[21]  L. Dressler,et al.  Emerging impact of flow cytometry in predicting recurrence and survival in breast cancer patients. , 1985, Journal of the National Cancer Institute.

[22]  K. Weber,et al.  Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments. , 1985, European journal of cell biology.

[23]  J. Bartek,et al.  PATTERNS OF EXPRESSION OF KERATIN 19 AS DETECTED WITH MONOCLONAL ANTIBODIES IN HUMAN BREAST TISSUES AND TUMOURS , 1985, International journal of cancer.

[24]  H. Preisler,et al.  Differences between labeling index and DNA histograms in assessing S-phase cells from a homogeneous group of chronic phase CML patients. , 1985, Cytometry.

[25]  J. Meyer,et al.  A comparison of human breast cancer cell kinetics measured by flow cytometry and thymidine labeling. , 1985, Laboratory investigation; a journal of technical methods and pathology.

[26]  Robert C. Leif,et al.  Convention on nomenclature for DNA cytometry , 1984 .

[27]  R. Moll,et al.  Immunohistochemical distinction of human carcinomas by cytokeratin typing with monoclonal antibodies. , 1984, The American journal of pathology.

[28]  M. Staquet,et al.  Reactivity pattern of a monoclonal antikeratin antibody (KL1). , 1983, The Journal of investigative dermatology.

[29]  P. Jap,et al.  Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology. , 1983, Laboratory investigation; a journal of technical methods and pathology.

[30]  R. Nagle,et al.  The use of antikeratin antibodies in the diagnosis of human neoplasms. , 1983, American journal of clinical pathology.

[31]  R. Moll,et al.  Complex cytokeratin polypeptide patterns observed in certain human carcinomas. , 1982, Differentiation; research in biological diversity.

[32]  Benjamin Geiger,et al.  The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells , 1982, Cell.

[33]  E. Lane.  Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization , 1982, The Journal of cell biology.

[34]  E. Lazarides.  Intermediate filaments as mechanical integrators of cellular space , 1980, Nature.