Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry.

We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.

[1]  E. Nordhoff,et al.  Direct mass spectrometric sequencing of low-picomole amounts of oligodeoxynucleotides with up to 21 bases by matrix-assisted laser desorption/ionization mass spectrometry , 1995 .

[2]  R. Griffey,et al.  Utility of organic bases for improved electrospray mass spectrometry of oligonucleotides. , 1995, Rapid communications in mass spectrometry : RCM.

[3]  K. Bleicher,et al.  Analysis of oligonucleotides using coupled high performance liquid chromatography-electrospray mass spectrometry , 1994 .

[4]  R. Bischoff,et al.  Negative electrospray ionization mass spectrometry of synthetic and chemically modified oligonucleotides. , 1994, Nucleic acids research.

[5]  C. Iden,et al.  Analysis of synthetic oligodeoxynucleotides containing modified components by electrospray ionization mass spectrometry. , 1994, Analytical biochemistry.

[6]  K. Bleicher,et al.  Various factors influencing the signal intensity of oligonucleotides in electrospray mass spectrometry. , 1994, Biological mass spectrometry.

[7]  U. Pieles,et al.  Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for the mass and sequence analysis of natural and modified oligonucleotides. , 1993, Nucleic acids research.

[8]  C. C. Hardin,et al.  Direct observation of a DNA quadruplex by electrospray ionization mass spectrometry. , 1993, Biological mass spectrometry.

[9]  R. Hettich,et al.  Structural Characterization of Normal and Modified Oligonucleotides by Matrix-assisted Laser Desorption Fourier Transform Mass Spectrometry , 1991, Journal of the American Society for Mass Spectrometry.

[10]  H. Blöcker,et al.  Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry. , 1982, Nucleic acids research.