Proteindesign für die Entwicklung von industriellen Mikroorganismen

The fast and efficient development of industrial microorganisms for the production of new compounds requires an integrated interplay of various approaches to manipulate cell metabolism from atomic to systems level. Techniques from systems and synthetic biology, like protein modeling and design, greatly enhance our capabilities of metabolic engineering. Here, we illustrate some current strategies and recent achievements of protein design in the context of metabolic engineering.

[1]  Chunhui Li,et al.  Exploring the diversity of complex metabolic networks , 2005, Bioinform..

[2]  A. Zeng,et al.  Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli , 2013, Applied Microbiology and Biotechnology.

[3]  A. Zeng,et al.  Structural synthetic biotechnology: from molecular structure to predictable design for industrial strain development. , 2010, Trends in biotechnology.

[4]  P. Babbitt,et al.  Enzyme (re)design: lessons from natural evolution and computation. , 2009, Current opinion in chemical biology.

[5]  A. Zeng,et al.  Integrating molecular dynamics and co-evolutionary analysis for reliable target prediction and deregulation of the allosteric inhibition of aspartokinase for amino acid production. , 2011, Journal of biotechnology.

[6]  A. Zeng,et al.  Exploring the allosteric mechanism of dihydrodipicolinate synthase by reverse engineering of the allosteric inhibitor binding sites and its application for lysine production , 2013, Applied Microbiology and Biotechnology.

[7]  A. Zeng,et al.  Protein design in systems metabolic engineering for industrial strain development , 2013, Biotechnology journal.

[8]  A. Zeng,et al.  Coevolutionary Analysis Enabled Rational Deregulation of Allosteric Enzyme Inhibition in Corynebacterium glutamicum for Lysine Production , 2011, Applied and Environmental Microbiology.

[9]  Jeong Wook Lee,et al.  Systems metabolic engineering of microorganisms for natural and non-natural chemicals. , 2012, Nature chemical biology.

[10]  S. Picataggio,et al.  Potential impact of synthetic biology on the development of microbial systems for the production of renewable fuels and chemicals. , 2009, Current opinion in biotechnology.