Germination and Early Seedling Growth of High Andean Native Plants under Heavy Metal Stress

The development of large-scale mining activity along the Central Andes of Argentina (CAA) has generated significant amounts of waste materials containing heavy metals. Phytoremediation is a promising eco-friendly, low-cost, and effective technology for the removal of heavy metals. The present study aimed to identify two native dominant species from the CCA, Adesmia subterranea and A. pinifolia, as metal-tolerant plant species for the first time, by evaluating the germination and early seedling growth at different concentrations (ppm) of Cd (3, 4.5 and 6), Ni (150, 225 and 300), As (20, 30 and 40), and Hg (0.8, 1.2 and 1.6) Early seedling growth was found to be more sensitive to heavy metals than germination. Ni and As exhibited the greatest inhibitory effect on both species’ germination percentages. In contrast, with Cd and Hg, no inhibitory effect was recorded. Root length, metal tolerance index, and fresh and dry weight were stimulated with Hg. However, the phytotoxic effect was greater as the concentration of Ni, As, and Cd increased. As an overall conclusion, the order of toxicity for these species can be classified as Ni > As > Cd > Hg. Therefore, Adesmia species could be considered as candidates for phytoremediation of soils contaminated with Hg and low concentrations of Cd.

[1]  M. Carvalho,et al.  Changes in Chromosome Complement and Germination of Lettuce (Lactuca sativa L.) Exposed to Heavy Metal Stress , 2023, Water, Air and Soil Pollution.

[2]  Carolina Kalinhoff,et al.  Mercury Phytotoxicity and Tolerance in Three Wild Plants during Germination and Seedling Development , 2022, Plants.

[3]  R. Almeer,et al.  Plant-Derived Smoke Solution Alleviates Cellular Oxidative Stress Caused by Arsenic and Mercury by Modulating the Cellular Antioxidative Defense System in Wheat , 2022, Plants.

[4]  J. Castillo,et al.  Metal effects on germination and seedling development in closely-related halophyte species inhabiting different elevations along the intertidal gradient. , 2022, Marine pollution bulletin.

[5]  Hyojin Kim,et al.  Assessing the effects of accumulated Cd(II) on seed germination and root development of Arabidopsis thaliana , 2021, Applied Biological Chemistry.

[6]  M. Gavrilescu.  Enhancing phytoremediation of soils polluted with heavy metals. , 2021, Current opinion in biotechnology.

[7]  G. Owens,et al.  Suitability of Indian mustard genotypes for phytoremediation of mercury-contaminated sites , 2021 .

[8]  I. Ali,et al.  A critical review on the phytoremediation of heavy metals from environment: Performance and challenges. , 2021, Chemosphere.

[9]  Renu Sharma,et al.  Effect of heavy metals: An overview , 2021, Materials Today: Proceedings.

[10]  M. Bilgen,et al.  Effects of Some Heavy Metals on Germination and Seedling Growth of Sorghum , 2020 .

[11]  Renald Blundell,et al.  Heavy metal pollution in the environment and their toxicological effects on humans , 2020, Heliyon.

[12]  L. Yáñez-Espinosa,et al.  Effect of heavy metals on seed germination and seedling development of Nama aff. stenophylla collected on the slope of a mine tailing dump , 2020, International journal of phytoremediation.

[13]  Shaoyong Lu,et al.  Effect of soil mercury pollution on ginger (Zingiber officinale Roscoe): Growth, product quality, health risks and silicon mitigation. , 2020, Ecotoxicology and environmental safety.

[14]  Yunhua Xiao,et al.  Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils , 2020, Environmental Science and Pollution Research.

[15]  L. L. Martins,et al.  Effect of Cd, Cr, Cu, Mn, Ni, Pb and Zn on seed germination and seedling growth of two lettuce cultivars (Lactuca sativa L.) , 2020, Plant Physiology Reports.

[16]  G. Abbas,et al.  Effect of salinity on physiological, biochemical and photostabilizing attributes of two genotypes of quinoa (Chenopodium quinoa Willd.) exposed to arsenic stress. , 2020, Ecotoxicology and environmental safety.

[17]  B. Nedjimi.  Germination characteristics of Peganum harmala L. (Nitrariaceae) subjected to heavy metals: implications for the use in polluted dryland restoration , 2019, International Journal of Environmental Science and Technology.

[18]  S. S. Dhaliwal,et al.  Remediation techniques for removal of heavy metals from the soil contaminated through different sources: a review , 2019, Environmental Science and Pollution Research.

[19]  G. Cappai,et al.  Effects of zinc and lead on seed germination of Helichrysum microphyllum subsp. tyrrhenicum, a metal-tolerant plant , 2019, International Journal of Environmental Science and Technology.

[20]  Bedabrata Saha,et al.  Phytotoxicity of Cd and Zn on three popular Indian mustard varieties during germination and early seedling growth , 2019, Biocatalysis and Agricultural Biotechnology.

[21]  L. Carvalho,et al.  Physiological response of Cistus salviifolius L. to high arsenic concentrations , 2019, Environmental Geochemistry and Health.

[22]  M. Lominchar,et al.  Effects of mercury on the germination and growth of Quercus ilex L. seedlings , 2019, Environmental Science and Pollution Research.

[23]  C. Cameselle,et al.  Benefits of phytoremediation amended with DC electric field. Application to soils contaminated with heavy metals. , 2019, Chemosphere.

[24]  Y. Ok,et al.  Heavy metal-induced oxidative stress on seed germination and seedling development: a critical review , 2019, Environmental Geochemistry and Health.

[25]  Y. Nazzal,et al.  Assessment of heavy metals in roadside dust along the Abu Dhabi–Al Ain National Highway, UAE , 2019, Environmental Earth Sciences.

[26]  M. Gálvez,et al.  Copper Uptake by Adesmia atacamensis in a Mine Tailing in an Arid Environment , 2018 .

[27]  Xu Zhang,et al.  Physiological responses of Suaeda glauca and Arabidopsis thaliana in phytoremediation of heavy metals. , 2018, Journal of environmental management.

[28]  E. Muszyńska,et al.  Studies on lead and cadmium toxicity in Dianthus carthusianorum calamine ecotype cultivated in vitro. , 2018, Plant biology.

[29]  Evgenios Agathokleous,et al.  Environmental hormesis, a fundamental non-monotonic biological phenomenon with implications in ecotoxicology and environmental safety , 2018 .

[30]  Natasha,et al.  Arsenic Uptake, Toxicity, Detoxification, and Speciation in Plants: Physiological, Biochemical, and Molecular Aspects , 2018, International journal of environmental research and public health.

[31]  Liu Feng,et al.  Mercury accumulation plant Cyrtomium macrophyllum and its potential for phytoremediation of mercury polluted sites. , 2017, Chemosphere.

[32]  H. M. Hammad,et al.  Effect of water management and silicon on germination, growth, phosphorus and arsenic uptake in rice. , 2017, Ecotoxicology and environmental safety.

[33]  A. Hartmann,et al.  Heavy Metals Induce Iron Deficiency Responses at Different Hierarchic and Regulatory Levels1[OPEN] , 2017, Plant Physiology.

[34]  Sarita Tiwari,et al.  Comparative analysis of antioxidant response by Pteris vittata and Vetiveria zizanioides towards arsenic stress , 2017 .

[35]  L. Cabo,et al.  Effect of the combined addition of Zn and Pb on partitioning in sediments and their accumulation by the emergent macrophyte Schoenoplectus californicus , 2017, Environmental Science and Pollution Research.

[36]  W. Shen,et al.  Methane alleviates copper-induced seed germination inhibition and oxidative stress in Medicago sativa , 2017, BioMetals.

[37]  D. Ghosh,et al.  Evaluation of effectiveness of seed priming with selenium in rice during germination under arsenic stress. , 2016, Plant physiology and biochemistry : PPB.

[38]  A. Watson,et al.  Effect of heavy metals on seed germination and seedling growth of common ragweed and roadside ground cover legumes. , 2016, Environmental pollution.

[39]  Ping Wang,et al.  Challenges and opportunities in the phytoremediation of heavy metals contaminated soils: A review. , 2016, Ecotoxicology and environmental safety.

[40]  Nikhil Kumar.  Effect of Heavy Metals on Plants : An Overview , 2016 .

[41]  Stephen D. Ebbs,et al.  Growth of selected plant species in biosolids-amended mine tailings , 2015 .

[42]  Bangquan Huang,et al.  Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Eruca sativa Mill. , 2015 .

[43]  C. Gardi,et al.  Germination and Root Elongation Bioassays in Six Different Plant Species for Testing Ni Contamination in Soil , 2014, Bulletin of Environmental Contamination and Toxicology.

[44]  C. Rensing,et al.  Phytoremediation of Heavy and Transition Metals Aided by Legume-Rhizobia Symbiosis , 2014, International journal of phytoremediation.

[45]  E. Donati,et al.  Environmental Impact on Soil, Water and Plants from the Abandoned Pan de Azúcar Mine , 2013 .

[46]  M. S. Khan,et al.  Toxicity of Heavy Metals to Legumes and Bioremediation , 2012, Springer Vienna.

[47]  Meetu Gupta,et al.  Comparative biochemical and RAPD analysis in two varieties of rice (Oryza sativa) under arsenic stress by using various biomarkers. , 2012, Journal of hazardous materials.

[48]  Z. Yang,et al.  Mercury toxicity, molecular response and tolerance in higher plants , 2012, BioMetals.

[49]  I. Kranner,et al.  Metals and seeds: Biochemical and molecular implications and their significance for seed germination , 2011 .

[50]  M. Hussain,et al.  Toxic Effect of Nickel (Ni) on Growth and Metabolism in Germinating Seeds of Sunflower (Helianthus annuus L.) , 2011, Biological Trace Element Research.

[51]  Alexander Brenning,et al.  Minería y glaciares rocosos: impactos ambientales, antecedentes políticos y legales, y perspectivas futuras , 2010 .

[52]  R. Maier,et al.  Phytostabilization of Mine Tailings in Arid and Semiarid Environments—An Emerging Remediation Technology , 2007, Environmental health perspectives.

[53]  K. Reardon,et al.  Comparison of plant families in a greenhouse phytoremediation study on an aged polycyclic aromatic hydrocarbon-contaminated soil. , 2007, Journal of environmental quality.

[54]  F. Schwegler,et al.  Air Quality Management: A Mining Perspective , 2006 .

[55]  Yong-guan Zhu,et al.  Toxicity of arsenate and arsenite on germination, seedling growth and amylolytic activity of wheat. , 2005, Chemosphere.

[56]  C. Parera,et al.  Adesmia subterranea Clos Germination Physiology and Presowing Treatments , 2003 .

[57]  L. Toppi,et al.  Response to cadmium in higher plants , 1999 .

[58]  E. M. Carretero,et al.  La vegetación puneña en la provincia de Mendoza, Argentina , 1998 .

[59]  R. Probert,et al.  Seed germination responses to some environmental factors in the seagrass Zostera capricorni from eastern Australia , 1998 .

[60]  D. Wilkins.  THE MEASUREMENT OF TOLERANCE TO EDAPHIC FACTORS BY MEANS OF ROOT GROWTH , 1978 .

[61]  B. Yan,et al.  Leachability characteristic of heavy metals and associated health risk study in typical copper mining-impacted sediments. , 2019, Chemosphere.

[62]  Ashok J. Kumar,et al.  Effects of nickel chloride on germination and seedling growth of different wheat (Triticum aestivum L. em Thell.) cultivars , 2018 .

[63]  M. Iqbal,et al.  Toxic effects of lead and cadmium on germination and seedling growth of Albizia lebbeck (L.) Benth. , 2009 .

[64]  F. Han,et al.  Bioaccumulation and physiological effects of mercury in Pteris vittata and Nephrolepis exaltata , 2009, Ecotoxicology.