An AFLP‐based linkage map of Zoysiagrass (Zoysia japonica)

To construct an amplified-fragment length polymorphism (AFLP)-based molecular linkage map of zoysiagrass, the selfed progenies of a clone consisting of 78 individuals were analysed using 471 AFLP markers derived from 126 PstI/MseI primer combinations. Of these markers, 364 were grouped into 26 linkage groups. The maps covered a total length of 932.5 cM, with an average spacing of 2.6 cM between markers. This information proves useful for gene targeting, quantitative trait loci mapping, and marker-assisted selection in zoysiagrass.

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