Decoration of electrical conductive polyurethane‐polyaniline/polyvinyl alcohol matrixes with mussel‐inspired polydopamine for bone tissue engineering

Electrospinning is a versatile technology for the fabrication of nanofibrous matrixes to regenerate defects. This study aims to develop a functionalized and electroconductive polymeric matrix to improve rat bone marrow mesenchymal stem cell adhesion, proliferation, and differentiation. Herein, the influence of the chemical composition of the substrate on homogeneous modification of the surface with mussel‐inspired polydopamine (PDA) is focused. Accordingly, the deposition of PDA on the surface was proved by Fourier transform infrared spectroscopy. Morphologies of the scaffolds demonstrated homogeneous decoration of the polyvinyl alcohol (PVA)/polyurethane (PU)‐polyaniline (PANI) matrixes with PDA, while a lower density of mussel‐inspired polymer was observed in bare PU‐PANI constructs. Although uniform and dense precipitation of PDA reduced conductivity of scaffolds 1.2 times compared with the samples with a low density of the PDA, 1.1 and 1.2 times enhancement in tensile strength and Young's modulus, respectively, were the strength of the applied process, especially in bone tissue engineering area. Contact angle measurements demonstrated about two times reduction in measured values, which shows improvement in hydrophilicity of PDA‐modified PVA/PU‐PANI fibers compared with PDA‐coated PU‐PANI ones. Swelling ratio and mass loss ratio calculations revealed enhancement in measured values as a function of homogeneous and dense coating, which arise from hydrophilicity of the polymeric substrate. The bioactivity test indicated that a dense layer of PDA strongly supports formations of hydroxyapatite‐like crystals. Moreover, homogeneous decoration of conductive matrixes with PDA showed suitable cell viability, adhesion, and spreading while cell‐scaffolds interactions improved under electrical stimulation. Higher expression of alkaline phosphatase and secretion of Collagen I under the electrical field proved the applicability of modified electroconductive scaffolds for further preclinical and clinical studies to introduce as a reconstructive bone substitute.

[1]  G. Prabu,et al.  A Novel Profiled Multi-Pin Electrospinning System for Nanofiber Production and Encapsulation of Nanoparticles into Nanofibers , 2020, Scientific Reports.

[2]  L. Leppik,et al.  Electrical stimulation in bone tissue engineering treatments , 2020, European Journal of Trauma and Emergency Surgery.

[3]  P. Zarrintaj,et al.  Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering , 2019, Biomolecules.

[4]  A. Zamanian,et al.  Fabrication and characterisation of super-paramagnetic responsive PLGA-gelatine-magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation. , 2019, IET nanobiotechnology.

[5]  Zhi-gang Chen,et al.  Bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes for robust bone regeneration , 2019, NPG Asia Materials.

[6]  E. García-Hernández,et al.  Characterization of Copolymer Based in Polyurethane and Polyaniline (PU/PANI) , 2019, Journal of the Mexican Chemical Society.

[7]  Yong Liu,et al.  Polyaniline Functionalized Graphene Nanoelectrodes for the Regeneration of PC12 Cells via Electrical Stimulation , 2019, International journal of molecular sciences.

[8]  S. Jaganathan,et al.  Engineering electrospun multicomponent polyurethane scaffolding platform comprising grapeseed oil and honey/propolis for bone tissue regeneration , 2018, PloS one.

[9]  S. Khorshidi,et al.  Particle-coated electrospun scaffold: A semi-conductive drug eluted scaffold with layered fiber/particle arrangement. , 2018, Journal of biomedical materials research. Part A.

[10]  Tairong Kuang,et al.  Electrospun poly (butylene succinate)/cellulose nanocrystals bio-nanocomposite scaffolds for tissue engineering: Preparation, characterization and in vitro evaluation , 2018, Polymer Testing.

[11]  Jae Young Lee,et al.  Electrically Conductive Polydopamine-Polypyrrole as High Performance Biomaterials for Cell Stimulation in Vitro and Electrical Signal Recording in Vivo. , 2018, ACS applied materials & interfaces.

[12]  Vivek Damodar Ranjan,et al.  Three-dimensional electrical conductive scaffold from biomaterial-based carbon microfiber sponge with bioinspired coating for cell proliferation and differentiation , 2018, Carbon.

[13]  F. Oktar,et al.  Development of Amoxicillin-Loaded Electrospun Polyurethane/Chitosan/ $\beta$ -Tricalcium Phosphate Scaffold for Bone Tissue Regeneration , 2018, IEEE Transactions on NanoBioscience.

[14]  A. Zamanian,et al.  Microwave-induced rapid formation of biomimetic hydroxyapatite coating on gelatin-siloxane hybrid microspheres in 10X-SBF solution , 2018 .

[15]  A. Zamanian,et al.  A Two‐Stage Kinetics Model for Polydopamine Layer Growth , 2018 .

[16]  Karen Low,et al.  Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells. , 2018, Acta biomaterialia.

[17]  C. Park,et al.  Bio-inspired hybrid scaffold of zinc oxide-functionalized multi-wall carbon nanotubes reinforced polyurethane nanofibers for bone tissue engineering , 2017 .

[18]  P. Ma,et al.  Electrospun conductive nanofibrous scaffolds for engineering cardiac tissue and 3D bioactuators. , 2017, Acta biomaterialia.

[19]  Jaehwan Kim,et al.  A novel approach for fabricating highly tunable and fluffy bioinspired 3D poly(vinyl alcohol) (PVA) fiber scaffolds. , 2017, Nanoscale.

[20]  E. Lizundia,et al.  Tuneable hydrolytic degradation of poly(l-lactide) scaffolds triggered by ZnO nanoparticles. , 2017, Materials science & engineering. C, Materials for biological applications.

[21]  Baoqing Yu,et al.  Enhance the Bioactivity and Osseointegration of the Polyethylene‐Terephthalate‐Based Artificial Ligament via Poly(Dopamine) Coating with Mesoporous Bioactive Glass   , 2017 .

[22]  W. Deng,et al.  Electroresponsive and cell-affinitive polydopamine/polypyrrole composite microcapsules with a dual-function of on-demand drug delivery and cell stimulation for electrical therapy , 2017 .

[23]  Ce Wang,et al.  Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation. , 2017, Materials science & engineering. C, Materials for biological applications.

[24]  Hyungsuk Lee,et al.  Highly conductive and hydrated PEG-based hydrogels for the potential application of a tissue engineering scaffold , 2016 .

[25]  M. Floren,et al.  Mussel-inspired polydopamine for bio-surface functionalization , 2016, Biosurface and biotribology.

[26]  Guangting Han,et al.  Electromechanical deformation sensors based on polyurethane/polyaniline electrospinning nanofibrous mats , 2016 .

[27]  R. Meneghello,et al.  Powder-based 3D printing for bone tissue engineering. , 2016, Biotechnology advances.

[28]  Jerry C. Hu,et al.  Cell-based tissue engineering strategies used in the clinical repair of articular cartilage. , 2016, Biomaterials.

[29]  F. Variola,et al.  Evaluation of the direct effects of poly(dopamine) on the in vitro response of human osteoblastic cells. , 2016, Journal of materials chemistry. B.

[30]  M. Soleimani,et al.  The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells , 2016, Bioprocess and Biosystems Engineering.

[31]  K. Khalil,et al.  Fabrication of novel high performance ductile poly(lactic acid) nanofiber scaffold coated with poly(vinyl alcohol) for tissue engineering applications. , 2016, Materials science & engineering. C, Materials for biological applications.

[32]  Jianjun Yang,et al.  Surface modification of zirconia with polydopamine to enhance fibroblast response and decrease bacterial activity in vitro: A potential technique for soft tissue engineering applications. , 2015, Colloids and surfaces. B, Biointerfaces.

[33]  C. Schmidt,et al.  Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Nanofibers Enhances their Differentiation toward Osteogenic Outcomes. , 2015, Macromolecular rapid communications.

[34]  L. Shao,et al.  Mussel-Inspired Hybrid Coatings that Transform Membrane Hydrophobicity into High Hydrophilicity and Underwater Superoleophobicity for Oil-in-Water Emulsion Separation. , 2015, ACS applied materials & interfaces.

[35]  Xing Zhang,et al.  Improving osteointegration and osteogenesis of three-dimensional porous Ti6Al4V scaffolds by polydopamine-assisted biomimetic hydroxyapatite coating. , 2015, ACS applied materials & interfaces.

[36]  A. Boccaccini,et al.  Tissue engineering of electrically responsive tissues using polyaniline based polymers: a review. , 2014, Biomaterials.

[37]  L. Ge,et al.  Polydopamine-coated paper-stack nanofibrous membranes enhancing adipose stem cells' adhesion and osteogenic differentiation. , 2014, Journal of materials chemistry. B.

[38]  Xuehong Lu,et al.  Shape memory polyurethane with polydopamine-coated nanosheets: Simultaneous enhancement of recovery stress and strain recovery ratio and the underlying mechanisms , 2014 .

[39]  Lehui Lu,et al.  Polydopamine and its derivative materials: synthesis and promising applications in energy, environmental, and biomedical fields. , 2014, Chemical reviews.

[40]  A. Albertsson,et al.  Biodegradable and electrically conducting polymers for biomedical applications , 2013 .

[41]  Shuping Peng,et al.  Fabrication of porous polyvinyl alcohol scaffold for bone tissue engineering via selective laser sintering , 2013, Biofabrication.

[42]  Antonios G Mikos,et al.  Strategies for controlled delivery of growth factors and cells for bone regeneration. , 2012, Advanced drug delivery reviews.

[43]  Young Min Shin,et al.  Mussel-inspired surface modification of poly(L-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells. , 2012, Colloids and surfaces. B, Biointerfaces.

[44]  M. Mozafari,et al.  Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering , 2012, International journal of nanomedicine.

[45]  W. Tsai,et al.  Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering. , 2011, Acta biomaterialia.

[46]  Jinhong Jiang,et al.  Surface characteristics of a self-polymerized dopamine coating deposited on hydrophobic polymer films. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[47]  Azadeh Asefnejad,et al.  Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay , 2011, International journal of nanomedicine.

[48]  C. Tonda-Turo,et al.  Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior. , 2011, Journal of the mechanical behavior of biomedical materials.

[49]  R. Marchant,et al.  Design properties of hydrogel tissue-engineering scaffolds , 2011, Expert review of medical devices.

[50]  Myung-Hyun Ryou,et al.  Mussel‐Inspired Polydopamine‐Treated Polyethylene Separators for High‐Power Li‐Ion Batteries , 2011, Advanced materials.

[51]  Thomas A Einhorn,et al.  The biology of fracture healing. , 2011, Injury.

[52]  J. Lovmand,et al.  Polydopamine/liposome coatings and their interaction with myoblast cells. , 2011, ACS applied materials & interfaces.

[53]  F. O'Brien.  Biomaterials & scaffolds for tissue engineering , 2011 .

[54]  Yufeng Zheng,et al.  Electrospinning of PLGA/gelatin randomly-oriented and aligned nanofibers as potential scaffold in tissue engineering , 2010 .

[55]  Wayne A Morrison,et al.  Strategies in cardiac tissue engineering , 2010, ANZ journal of surgery.

[56]  Haeshin Lee,et al.  Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization , 2010 .

[57]  X Wang,et al.  Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method. , 2010, Acta biomaterialia.

[58]  J. A. Posada,et al.  Preparation and Properties of Polyurethanes based on Castor Oil Chemically Modified with Yucca Starch Glycoside , 2009 .

[59]  H. Tamura,et al.  The Mechanical and Biological Properties of Chitosan Scaffolds for Tissue Regeneration Templates Are Significantly Enhanced by Chitosan from Gongronella butleri , 2009, Materials.

[60]  S. Britland,et al.  Poly(vinyl alcohol) Hydrogel as a Biocompatible Viscoelastic Mimetic for Articular Cartilage , 2008, Biotechnology progress.

[61]  Chuanqiang Zhou,et al.  Fabrication of polyaniline with hierarchical structures in alkaline solution , 2008 .

[62]  Fenglin Yang,et al.  Preparation and characterization of hydrophilic modification of polypropylene non-woven fabric by dip-coating PVA (polyvinyl alcohol) , 2008 .

[63]  Q. Wei,et al.  Structural characterization and dynamic water adsorption of electrospun polyamide6/montmorillonite nanofibers , 2008 .

[64]  P. Gatenholm,et al.  Electrospinning of highly porous scaffolds for cartilage regeneration. , 2008, Biomacromolecules.

[65]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[66]  Yen Wei,et al.  Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications. , 2006, Biomaterials.

[67]  Yen Wei,et al.  Polyaniline, an electroactive polymer, supports adhesion and proliferation of cardiac myoblasts , 2006, Journal of biomaterials science. Polymer edition.

[68]  James A. Johnson,et al.  Mechanical properties of cancellous bone of the distal humerus. , 2005, Clinical biomechanics.

[69]  P. N. Lisboa-Filho,et al.  Polyaniline/polyurethane networks. II. A spectroscopic study , 2005 .

[70]  Gary T. Howard,et al.  Biodegradation of polyurethane: a review , 2002 .

[71]  Xing‐dong Zhang,et al.  A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. , 1999, Biomaterials.

[72]  S D Cook,et al.  In vivo performance of a modified CSTi dental implant coating. , 1998, The International journal of oral & maxillofacial implants.

[73]  S F Hulbert,et al.  Potential of ceramic materials as permanently implantable skeletal prostheses. , 1970, Journal of biomedical materials research.

[74]  A. Zamanian,et al.  A bioinspired 3D shape olibanum‐collagen‐gelatin scaffolds with tunable porous microstructure for efficient neural tissue regeneration , 2019, Biotechnology progress.

[75]  A. Zamanian,et al.  A facile method to synthesize mussel-inspired polydopamine nanospheres as an active template for in situ formation of biomimetic hydroxyapatite. , 2019, Materials science & engineering. C, Materials for biological applications.

[76]  S. Bertoldi,et al.  Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration. , 2018, Materials science & engineering. C, Materials for biological applications.

[77]  Wei Song,et al.  Achieving ultrasensitive in vivo detection of bone crack with polydopamine-capsulated surface-enhanced Raman nanoparticle. , 2017, Biomaterials.

[78]  Chi-Chang Lin,et al.  Osteogenesis of human adipose-derived stem cells on poly(dopamine)-coated electrospun poly(lactic acid) fiber mats. , 2016, Materials science & engineering. C, Materials for biological applications.

[79]  Amit Bandyopadhyay,et al.  Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds , 2016, Annals of Biomedical Engineering.

[80]  N. Chanunpanich,et al.  Polycarpolactone Fibers Gellation with Gelatin Ground Substance: Engineered Skin Extracellular Matrix Aims for Using as Tissue Engineering Skin , 2014 .

[81]  Paulo Jorge Da Silva bartolo,et al.  Biofabrication Strategies for Tissue Engineering , 2011 .

[82]  J. Waite.  Mussel power. , 2008, Nature materials.

[83]  T. Einhorn,et al.  Biomechanics of Bone , 2002 .

[84]  F. Linde,et al.  The effect of constraint on the mechanical behaviour of trabecular bone specimens. , 1989, Journal of biomechanics.