Protein adsorption onto ceramic surfaces.

Ceramics seldom have been used as blood-contacting materials. However, alumina ceramic (Al2O3) and polyethylene are incorporated into the pivot bearings of the Gyro centrifugal blood pump. This material combination was chosen based on the high durability of the materials. Due to the stagnant flow that often occurs in a continuous flow condition inside a centrifugal pump, pivot bearing system is extremely critical. To evaluate the thombogenicity of pivot bearings in the Gyro pump, this study sought to investigate protein adsorption, particularly albumin, IgG, fibrinogen, and fibronectin onto ceramic surfaces. Al2O3 and silicon carbide ceramic (SiC) were compared with polyethylene (PE) and polyvinylchloride (PVC). Bicinchoninic acid (BCA) protein assay revealed that the amount of adsorbed proteins onto Al2O3 and SiC was significantly less than that on PVC. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that numerous proteins adsorbed onto PVC compared to PE, Al2O3, and SiC. Identification of adsorbed proteins by Western immunoblotting revealed that the adsorption of albumin was similar on all four materials tested. Western immunoblotting also indicated lesser amounts of IgG, fibrinogen, and fibronectin on Al2O3 and SiC than on PE and PVC. In conclusion, ceramics (Al2O3 and SiC) are expected to be thromboresistant from the viewpoint of protein adsorption.

[1]  Y. Ohara,et al.  Hemolytic characteristics of a pivot bearing supported Gyro centrifugal pump (C1E3) simulating various clinical applications. , 1996, Artificial organs.

[2]  J. Sixma,et al.  Platelet adhesion to fibronectin in flow: the importance of von Willebrand factor and glycoprotein Ib. , 1995, Blood.

[3]  E Okamoto,et al.  Development of a Fine Ceramic Heart Valve for Use as a Cardiac Prosthesis , 1986, ASAIO transactions.

[4]  T. Horbett,et al.  The effects of temperature and buffer on fibrinogen adsorption from blood plasma to glass. , 1991, Journal of biomaterials science. Polymer edition.

[5]  K Watanabe,et al.  The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress. , 1991, The Journal of clinical investigation.

[6]  J. Chesebro,et al.  Platelet Deposition In Extracardiac Conduits In Humans: A Noninvasive Quantification , 1981, Thrombosis and Haemostasis.

[7]  K. Takaoka,et al.  Polyethylene sockets and alumina ceramic heads in cemented total hip arthroplasty. A ten-year study. , 1995, The Journal of bone and joint surgery. British volume.

[8]  S Takatani,et al.  Evaluation of the Wear of the Pivot Bearing in the Gyro C1E3 Pump. , 1996, Artificial organs.

[9]  L. Parise,et al.  LA Fitzgerald The platelet membrane glycoprotein IIb-IIIa complex , 1988 .

[10]  J. Brash,et al.  Identification of plasma proteins adsorbed to hemodialyzers during clinical use. , 1989, Journal of biomedical materials research.

[11]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[12]  J. Brash,et al.  Platelet-foreign surface interactions: release of granule constituents from adherent platelets. , 1978, Journal of biomedical materials research.

[13]  G. Thun,et al.  Immunodiffusion and immunohistochemical investigations on the reactivity of oxide ceramic middle-ear implants. , 1993, ORL; journal for oto-rhino-laryngology and its related specialties.

[14]  G. Ryu,et al.  Effect of shear stress on fibrinogen adsorption and its conformational change. , 1995, ASAIO journal.

[15]  G Damm,et al.  Baylor Gyro Pump: a completely seal-less centrifugal pump aiming for long-term circulatory support. , 2008, Artificial organs.

[16]  L. A. Genova,et al.  The interaction of blood proteins with alpha-alumina. , 1994, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[17]  G. Tansley,et al.  Development of a ceramic conduit valve prosthesis for corrective cardiovascular surgery. , 1995, Biomaterials.

[18]  Y Nosé,et al.  Development and evaluation of antithrombogenic centrifugal pump: the Baylor C-Gyro Pump Eccentric Inlet Port Model. , 1994, Artificial organs.

[19]  G Damm,et al.  An ultimate, compact, seal-less centrifugal ventricular assist device: Baylor C-Gyro pump. , 1994, Artificial organs.

[20]  W. A. Maxwell,et al.  Identification of intraocular lens-adsorbed proteins in mammalian in vitro and in vivo systems. , 1994, Archives of ophthalmology.

[21]  George Damm,et al.  Development of a Pivot Bearing Supported Sealless Centrifugal Pump for Ventricular Assist. , 1996, Artificial organs.

[22]  J. Davidson,et al.  Characteristics of metal and ceramic total hip bearing surfaces and their effect on long-term ultra high molecular weight polyethylene wear. , 1993, Clinical orthopaedics and related research.