Influenza A virus molecularly imprinted polymers and their application in virus sub-type classification.

In this work, we apply a molecular imprinting strategy as a screening protocol for different influenza A subtypes, namely H5N1, H5N3, H1N1, H1N3 and H6N1. Molecularly imprinted polymers for each of these subtypes lead to appreciable sensor characteristics on a quartz crystal microbalance leading to detection limits as low as 105 particles per ml. Selectivity studies indicate that each virus is preferably incorporated by its own MIP. Recognition in most cases is dominated by the neuraminidase residue rather than the hemagglutinin. Multivariate analysis shows that the sensor responses can be correlated with the differences in hemagglutinin and neuraminidase patterns from databases. This allows for virus subtype characterization and thus rapid screening.

[1]  Franz L Dickert,et al.  Synthetic receptors for selectively detecting erythrocyte ABO subgroups. , 2009, Analytica chimica acta.

[2]  R. Webster,et al.  Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus , 1998, The Lancet.

[3]  M. Peiris,et al.  Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus , 1998, The Lancet.

[4]  Kiattawee Choowongkomon,et al.  Surface molecular imprints of WGA lectin as artificial receptors for mass-sensitive binding studies , 2011, Analytical and bioanalytical chemistry.

[5]  K. Marx,et al.  Quartz crystal microbalance: a useful tool for studying thin polymer films and complex biomolecular systems at the solution-surface interface. , 2003, Biomacromolecules.

[6]  Franz L. Dickert,et al.  Mass-sensitive detection of cells, viruses and enzymes with artificial receptors , 2003 .

[7]  A. Nizam,et al.  Containing Pandemic Influenza at the Source , 2005, Science.

[8]  G. Mustafa,et al.  Quartz crystal microbalance sensor based on affinity interactions between organic thiols and molybdenum disulfide nanoparticles , 2012 .

[9]  Monique Mauzac,et al.  Molecular imprinting : State of the art and perspectives , 2005 .

[10]  Olof Ramström,et al.  The Emerging Technique of Molecular Imprinting and Its Future Impact on Biotechnology , 1996, Bio/Technology.

[11]  Arunas Ramanavicius,et al.  Molecularly imprinted polypyrrole-based synthetic receptor for direct detection of bovine leukemia virus glycoproteins. , 2004, Biosensors & bioelectronics.

[12]  Maryam Tabrizian,et al.  Biomolecule imprinting: Developments in mimicking dynamic natural recognition systems , 2008 .

[13]  J. Z. Hilt,et al.  Configurational biomimesis in drug delivery: molecular imprinting of biologically significant molecules. , 2004, Advanced drug delivery reviews.

[14]  Shoufang Xu,et al.  Recent advances in molecular imprinting technology: current status, challenges and highlighted applications. , 2011, Chemical Society reviews.

[15]  Christian Le Grimellec,et al.  Organization of influenza A virus envelope at neutral and low pH. , 2010, The Journal of general virology.

[16]  B. Rigas,et al.  Potentiometric sensors based on surface molecular imprinting: Detection of cancer biomarkers and viruses , 2010 .

[17]  G. Sauerbrey.  Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung , 1959 .

[18]  Franz L Dickert,et al.  Bioimprinted QCM sensors for virus detection—screening of plant sap , 2004, Analytical and Bioanalytical Chemistry.

[19]  F. Dickert,et al.  Bioimprinting of polymers and sol-gel phases. Selective detection of yeasts with imprinted polymers. , 2002, Analytical chemistry.

[20]  Buddy D. Ratner,et al.  Template-imprinted nanostructured surfaces for protein recognition , 1999, Nature.

[21]  Quan Cheng,et al.  Detection of influenza virus: traditional approaches and development of biosensors , 2005, Analytical and bioanalytical chemistry.

[22]  Günter Wulff,et al.  Enzyme-analogue built polymers and their use for the resolution of racemates , 1973 .

[23]  James M Aramini,et al.  Structures of influenza A proteins and insights into antiviral drug targets , 2010, Nature Structural &Molecular Biology.

[24]  J. Gordon,et al.  Frequency of a quartz microbalance in contact with liquid , 1985 .

[25]  G. Robertson,et al.  Evaluation of the recognition ability of molecularly imprinted materials by surface plasmon resonance (SPR) spectroscopy , 2003 .

[26]  Ziping Wei,et al.  Improved particle counting and size distribution determination of aggregated virus populations by asymmetric flow field‐flow fractionation and multiangle light scattering techniques , 2011, Biotechnology progress.

[27]  A. Merkoçi,et al.  New materials for electrochemical sensing IV. Molecular imprinted polymers , 2002 .

[28]  Bernd Becker,et al.  A survey of the 2006–2009 quartz crystal microbalance biosensor literature , 2011, Journal of molecular recognition : JMR.

[29]  R. Lamb,et al.  Influenza virus hemagglutinin and neuraminidase cytoplasmic tails control particle shape , 1997, The EMBO journal.

[30]  J. Taubenberger,et al.  1918 Influenza: the Mother of All Pandemics , 2006, Emerging infectious diseases.

[31]  Prasert Auewarakul,et al.  Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. , 2005, The Journal of general virology.

[32]  Peter A. Lieberzeit,et al.  Artificial Antibodies for Bioanalyte Detection—Sensing Viruses and Proteins , 2006 .

[33]  Jeffrey H. Chuang,et al.  A molecular-imprint nanosensor for ultrasensitive detection of proteins. , 2010, Nature nanotechnology.

[34]  Theo M Bestebroer,et al.  Airborne Transmission of Influenza A/H5N1 Virus Between Ferrets , 2012, Science.

[35]  Libo Dong,et al.  Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus. , 2009, The New England journal of medicine.

[36]  Sebastian Bonhoeffer,et al.  This PDF file includes: SOM Text , 2022 .

[37]  Hideo Goto,et al.  Avian flu: Isolation of drug-resistant H5N1 virus , 2005, Nature.

[38]  G. Wulff.  Molecular Imprinting in Cross‐Linked Materials with the Aid of Molecular Templates— A Way towards Artificial Antibodies , 1995 .

[39]  J. Yewdell,et al.  Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection , 2011, The Journal of experimental medicine.

[40]  Frederick Hayden,et al.  Developing new antiviral agents for influenza treatment: what does the future hold? , 2009, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[41]  Gabriele Neumann,et al.  H5N1 influenza viruses: outbreaks and biological properties , 2010, Cell Research.

[42]  Sergey A. Piletsky,et al.  Electrochemical Sensors Based on Molecularly Imprinted Polymers , 2002 .

[43]  Nicholas A Peppas,et al.  Critical review and perspective of macromolecularly imprinted polymers. , 2012, Acta biomaterialia.

[44]  Noriaki Hara,et al.  SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles. , 2005, Analytical chemistry.

[45]  A. Osterhaus,et al.  Global Patterns of Influenza A Virus in Wild Birds , 2006, Science.

[46]  Franz L Dickert,et al.  Sensing picornaviruses using molecular imprinting techniques on a quartz crystal microbalance. , 2009, Analytical chemistry.

[47]  Alan J. Hay,et al.  Crystal structures of oseltamivir-resistant influenza virus neuraminidase mutants , 2008, Nature.

[48]  Keiji Fukuda,et al.  Detection of Antibody to Avian Influenza A (H5N1) Virus in Human Serum by Using a Combination of Serologic Assays , 1999, Journal of Clinical Microbiology.

[49]  R. Ruigrok,et al.  Changes in the morphology of influenza particles induced at low pH , 2005, Archives of Virology.

[50]  Sergey A. Piletsky,et al.  MIP sensors – the electrochemical approach , 2012, Analytical and Bioanalytical Chemistry.

[51]  Peter A Lieberzeit,et al.  Detection of viruses with molecularly imprinted polymers integrated on a microfluidic biochip using contact-less dielectric microsensors. , 2009, Lab on a chip.

[52]  Mark Wolff,et al.  Safety and immunogenicity of an inactivated subvirion influenza A (H5N1) vaccine. , 2006, The New England journal of medicine.

[53]  G. Sauerbrey,et al.  Use of quartz vibration for weighing thin films on a microbalance , 1959 .

[54]  M. Biggerstaff,et al.  H1N1 2009 influenza virus infection during pregnancy in the USA , 2009, The Lancet.

[55]  V. Torchilin,et al.  Targeted polymeric micelles for delivery of poorly soluble drugs , 2004, Cellular and Molecular Life Sciences CMLS.

[56]  Derek J Smith,et al.  Predictability and Preparedness in Influenza Control , 2006, Science.

[57]  Sergey A Piletsky,et al.  Molecularly imprinted polymers in clinical diagnostics--future potential and existing problems. , 2006, Medical engineering & physics.

[58]  Laura Anfossi,et al.  A connection between the binding properties of imprinted and nonimprinted polymers: a change of perspective in molecular imprinting. , 2012, Journal of the American Chemical Society.

[59]  B. Sellergren,et al.  Direct Drug Determination by Selective Sample Enrichment on an Imprinted Polymer , 1994 .

[60]  L. Andersson.  Selective solid-phase extraction of bio- and environmental samples using molecularly imprinted polymers , 2003, Bioseparation.

[61]  James N Culver,et al.  Molecularly imprinted polymers for tobacco mosaic virus recognition. , 2006, Biomaterials.

[62]  Klaus Mosbach,et al.  Drug assay using antibody mimics made by molecular imprinting , 1993, Nature.

[63]  M. Fukuda,et al.  High Susceptibility of Human Dendritic Cells to Avian Influenza H5N1 Virus Infection and Protection by IFN-α and TLR Ligands1 , 2007, The Journal of Immunology.

[64]  J. Taubenberger,et al.  The pathology of influenza virus infections. , 2008, Annual review of pathology.