Chromosomal Amplification of the Escherichia coli lipB Region Confers High-Level Resistance to Selenolipoic Acid

ABSTRACT One of the mutants (slr7 mutant) of a wild-type Escherichia coli strain resistant to selenolipoic acid reported previously (K. E. Reed, T. W. Morris, and J. E. Cronan, Jr., Proc. Natl. Acad. Sci. USA 91:3720-3724, 1994) unexpectedly grew on minimal medium following transductional introduction of a lipA null mutation. We report that the slr7 strain carries a duplication of the lip chromosomal region that causes the phenotype of the mutant strain.

[1]  T. Grundström,et al.  Isolation and characterization of DNA repetitions carrying the chromosomal β-lactamase gene of Escherichia coli K-12 , 1979, Molecular and General Genetics MGG.

[2]  J. R. Guest,et al.  Lipoic acid content of Escherichia coli and other microorganisms , 1975, Archives of Microbiology.

[3]  U. Henning,et al.  Der Einfluss von Suppressor-Gen-Mutation und intragenischer Reversion auf Mutationsorte vom 0°-Typ , 1964, Zeitschrift für Vererbungslehre.

[4]  R..  Tandem Genetic Duplications in Salmonella typhimurium : Amplification of the Histidine Operon , 2003 .

[5]  G. R. Smith,et al.  Homologous recombination near and far from DNA breaks: alternative roles and contrasting views. , 2001, Annual review of genetics.

[6]  J. Cronan,et al.  Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein. , 2000, Biochemistry.

[7]  R. Perham,et al.  Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions. , 2000, Annual review of biochemistry.

[8]  T. Galitski,et al.  Rearrangements of the Bacterial Chromosome : Formation and Applications , 1999 .

[9]  J. Cronan,et al.  A New Metabolic Link , 1997, The Journal of Biological Chemistry.

[10]  T. Morris,et al.  Purification and properties of the lipoate protein ligase of Escherichia coli. , 1995, The Biochemical journal.

[11]  T. Morris,et al.  Lipoic acid metabolism in Escherichia coli: the lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein , 1995, Journal of bacteriology.

[12]  T. Morris,et al.  Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. Molecular cloning and characterization of the lplA gene and gene product. , 1994, The Journal of biological chemistry.

[13]  T. Morris,et al.  Mutants of Escherichia coli K-12 that are resistant to a selenium analog of lipoic acid identify unknown genes in lipoate metabolism. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Cronan,et al.  Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes , 1993, Journal of bacteriology.

[15]  J. Cronan,et al.  Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system , 1991, Journal of bacteriology.

[16]  R. Perham.  Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein. , 1991, Biochemistry.

[17]  R. Gumport,et al.  Genetic analysis of Escherichia coli integration host factor interactions with its bacteriophage lambda H' recognition site , 1991, Journal of bacteriology.

[18]  F. de la Cruz,et al.  pACYC184-derived cloning vectors containing the multiple cloning site and lacZ alpha reporter gene of pUC8/9 and pUC18/19 plasmids. , 1988, Gene.

[19]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[20]  R. M. Oliver,et al.  Evidence for a multiple random coupling mechanism in the alpha-ketoglutarate dehydrogenase multienzyme complex of Escherichia coli: a computer model analysis. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Guyer.  Uses of the transposon gamma delta in the analysis of cloned genes. , 1983, Methods in enzymology.

[22]  R. Perham,et al.  Lipoic acid residues in a take-over mechanism for the pyruvate dehydrogenase multienzyme complex of Escherichia coli. , 1981, Biochemical Journal.

[23]  G. Roberts,et al.  Limited proteolysis and proton n.m.r. spectroscopy of the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli. , 1981, The Biochemical journal.

[24]  L. Reed,et al.  Use of trypsin and lipoamidase to study the role of lipoic acid moieties in the pyruvate and alpha-ketoglutarate dehydrogenase complexes of Escherichia coli. , 1981, Biochemistry.

[25]  S. Normark,et al.  Recombination between short DNA homologies causes tandem duplication , 1981, Nature.

[26]  W. G. Griffin,et al.  Kinetic analysis of the role of lipoic acid residues in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. , 1980, The Biochemical journal.

[27]  T. Grundström,et al.  Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12. , 1979, Molecular & general genetics : MGG.

[28]  A. Fersht,et al.  Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: mechanism for rate enhancement in a multimeric structure. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[29]  K. Angelides,et al.  Mechanism of action of the pyruvate dehydrogenase multienzyme complex from Escherichia coli. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[30]  E. Ohtsubo,et al.  Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB , 1978, Journal of bacteriology.

[31]  J R Roth,et al.  Tandem chromosomal duplications in Salmonella typhimurium: fusion of histidine genes to novel promoters. , 1978, Journal of molecular biology.

[32]  J R Roth,et al.  Tandem genetic duplications in phage and bacteria. , 1977, Annual review of microbiology.

[33]  John Carbon,et al.  A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome , 1976, Cell.

[34]  K. Low.  Escherichia coli K-12 F-prime factors, old and new , 1972, Bacteriological reviews.

[35]  J. Guest,et al.  Amber mutants of the -ketoglutarate dehydrogenase gene of Escherichia coli K12. , 1972, Journal of general microbiology.

[36]  P Berg,et al.  Transduction of Merodiploidy: Induced Duplication of Recipient Genes , 1969, Journal of bacteriology.

[37]  U. Henning,et al.  Amber mutants in the pyruvate dehydrogenase system of Escherichia coli K12. , 1965, Journal of molecular biology.