Effect of thidiazuron in germinating tamarind seedlings

SummaryTamarind, a multipurpose tropical tree species, is economically important for sustainable development of wasteland due to its hardy nature and adaptability to various agroclimatic ocnditions. Reports on in vitro morphogenesis in this species are limited, due to its recalcitrant and callogenic nature. To overcome these limitations, an attempt was made to induce meristematic activity in seedling explants. Seedlings were germinated in medium with or without thidiazuron (4.54, 9.08, 13.12, 18.16 μM). This growth regulator restricted the differentiation of the apical meristem to form shoots. It triggered proliferation of the meristematic tissue at the cotyledonary node and a large number of meristematic buds appeared in a ridial pattern around the node. The meristematic activity extended to the junction of the epicotyl and hypocotyl, giving rise to buds in the form of protuberances in all sides of the junction. These buds differentiated to form shoot primordia and subsequently to shoots in medium devoid of growth regulators. Plants developed by micrografting of these shoots on seedling-derived rootstocks survived in soil.

[1]  R. Geneve,et al.  Multiple shoot formation from cotyledonary node segments of Eastern redbud , 1997, Plant Cell, Tissue and Organ Culture.

[2]  B. Bongarten,et al.  High-frequency induction of adventitious shoots from hypocotyl segments ofLiquidambar styracifiua L. by thidiazuron , 1997, Plant Cell Reports.

[3]  U. Dhar,et al.  Micropropagation ofBauhinia vahlii Wight & Arnott — a leguminous liana , 1996, Plant Cell Reports.

[4]  Carl A. Huetteman,et al.  Thidiazuron: a potent cytokinin for woody plant tissue culture , 1993, Plant Cell, Tissue and Organ Culture.

[5]  P. Saxena,et al.  Somatic embryogenesis and organogenesis in peanut: The role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis , 1999, Plant Growth Regulation.

[6]  E. Wilhelm.  Micropropagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron , 1999, Plant Cell, Tissue and Organ Culture.

[7]  A. Gulati,et al.  Direct Organogenesis in Hypocotyl Cultures of Tamarindus Indica , 1998, Biologia Plantarum.

[8]  T. Davis,et al.  Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin , 1994, Plant Growth Regulation.

[9]  P. Saxena,et al.  Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut , 1992, Planta.

[10]  P. Saxena,et al.  Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L. , 1992, Plant Cell Reports.

[11]  P. Saxena,et al.  Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron , 1992, Planta.

[12]  P. K. Jaiwal,et al.  In vitro high frequency plant regeneration of a tree legume Tamarindus indica (L.) , 1991, Plant Cell Reports.

[13]  D. Gray,et al.  In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia) , 1991, Plant Cell, Tissue and Organ Culture.

[14]  T. Davis,et al.  In vitro regeneration of silktree (Albizzia julibrissin) from excised roots , 2004, Plant Cell, Tissue and Organ Culture.

[15]  U. Mehta.  Tissue Culture Studies in Tamarind (Tamarindous Indica L.), a Leguminous Tree Species , 2001 .

[16]  S. Hazra,et al.  Regeneration of plants via adventitious bud formation from mature zygotic embryo axis of tamarind (Tamarindus indica L. , 2000 .

[17]  A. Banerjee,et al.  Recovery of in vitro cotton shoots through micrografting. , 2000 .

[18]  U. Dhar,et al.  In vitro regeneration of a mature leguminous liana (Bauhinia vahlii Wight & Arnott) , 1999, Plant Cell Reports.

[19]  P. Saxena,et al.  Thidiazuron‐induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons , 1995 .

[20]  J. Preece,et al.  Plant regeneration from leaf explants of Rhododendron ‘P.J.M. Hybrids’ , 1991 .

[21]  Z. C. Li,et al.  DeltapH-Dependent Amino Acid Transport into Plasma Membrane Vesicles Isolated from Sugar Beet Leaves: I. Evidence for Carrier-Mediated, Electrogenic Flux through Multiple Transport Systems. , 1990, Plant physiology.

[22]  D. Durzan,et al.  Case histories : gymnosperms, angiosperms and palms , 1987 .

[23]  R. H. Zimmerman,et al.  Thidiazuron Stimulation of Apple Shoot Proliferation in Vitro , 1986, HortScience.

[24]  P. Sherrington,et al.  Plant propagation by tissue culture : handbook and directory of commercial laboratories , 1984 .

[25]  F. Skoog,et al.  A revised medium for the growth and bioassay with tobacco tissue culture , 1962 .

[26]  LU CHIN-YI.  THE USE OF THIDIAZURON IN TISSUE CULTURE 1 , 2022 .