Carnosol attenuates high glucose damage in human retinal endothelial cells through regulation of ERK/Nrf2/HO-1 pathway.

Carnosol is a natural compound with antioxidant properties. Based on this evidence, in the present study we investigated whether this compound can protect retinal vascular endothelium from hyperglycemic insult responsible for diabetic retinopathy development. We performed in vitro study on human retinal endothelial cells (HREC) cultured both in normal and high glucose conditions to assess the effects of carnosol on cell viability, Nrf2 expression, HO-1 activity, and ERK1/2 expression. HREC exposed to high glucose insult were treated with carnosol. Data indicated that carnosol treatment is able to induce HO-1 expression via Nrf2 activation and counteracts the damage elicited by high glucose. Further, carnosol activation of Nrf2/HO-1 signaling axis involves ERK1/2 pathway. These data confirm the therapeutic value of carnosol by suggesting its use to treat diabetic retinopathy.

[1]  Xiaotian Zhang,et al.  Irisin rescues diabetic cardiac microvascular injury via ERK1/2/Nrf2/HO-1 mediated inhibition of oxidative stress. , 2021, Diabetes research and clinical practice.

[2]  A. Sazili,et al.  Green Extraction of Bioactive Compounds from Plant Biomass and Their Application in Meat as Natural Antioxidant , 2021, Antioxidants.

[3]  Grazia Maugeri,et al.  Attenuation of High Glucose-Induced Damage in RPE Cells through p38 MAPK Signaling Pathway Inhibition , 2021, Frontiers in Pharmacology.

[4]  E. Tsiani,et al.  Anticancer Properties of Carnosol: A Summary of In Vitro and In Vivo Evidence , 2020, Antioxidants.

[5]  Grazia Maugeri,et al.  Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2‐mediated activation of the Nrf2/HO‐1 pathway , 2019, Journal of cellular physiology.

[6]  H. Jaeger,et al.  Stabilization of Crystalline Carotenoids in Carrot Concentrate Powders: Effects of Drying Technology, Carrier Material, and Antioxidants , 2019, Foods.

[7]  A. Dunne,et al.  Plant-Derived Polyphenols Modulate Human Dendritic Cell Metabolism and Immune Function via AMPK-Dependent Induction of Heme Oxygenase-1 , 2019, Front. Immunol..

[8]  W. Su,et al.  Hemin Promotes Corneal Allograft Survival Through the Suppression of Macrophage Recruitment and Activation. , 2018, Investigative ophthalmology & visual science.

[9]  T. Farkhondeh,et al.  Protective effects of carnosol against oxidative stress induced brain damage by chronic stress in rats , 2017, BMC Complementary and Alternative Medicine.

[10]  B. Song,et al.  Mechanistic Insights into Pathological Changes in the Diabetic Retina: Implications for Targeting Diabetic Retinopathy. , 2017, The American journal of pathology.

[11]  S. Govoni,et al.  Nanosystems based on siRNA silencing HuR expression counteract diabetic retinopathy in rat. , 2016, Pharmacological research.

[12]  X. Qiu,et al.  Carnosol protects against spinal cord injury through Nrf-2 upregulation , 2016, Journal of receptor and signal transduction research.

[13]  J. Dubois-Randé,et al.  Nrf2 activators modulate oxidative stress responses and bioenergetic profiles of human retinal epithelial cells cultured in normal or high glucose conditions. , 2015, Pharmacological research.

[14]  M. Okumura,et al.  Carnosol Is a Potent Lung Protective Agent: Experimental Study on Mice. , 2015, Transplantation proceedings.

[15]  Synan F. AbuQamar,et al.  Carnosol Induces ROS-Mediated Beclin1-Independent Autophagy and Apoptosis in Triple Negative Breast Cancer , 2014, PloS one.

[16]  R. Foresti,et al.  Heme oxygenase-1 as a target for drug discovery. , 2014, Antioxidants & redox signaling.

[17]  J. Dubois-Randé,et al.  Small molecule activators of the Nrf2-HO-1 antioxidant axis modulate heme metabolism and inflammation in BV2 microglia cells. , 2013, Pharmacological research.

[18]  S. Salomone,et al.  Fortified Extract of Red Berry, Ginkgo biloba, and White Willow Bark in Experimental Early Diabetic Retinopathy , 2013, Journal of diabetes research.

[19]  Jia-xu Chen,et al.  The antioxidant effect of carnosol in bovine aortic endothelial cells is mainly mediated via estrogen receptor α pathway. , 2012, Biological & pharmaceutical bulletin.

[20]  Jen-kun Lin,et al.  Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-kappaB in mouse macrophages. , 2002, Carcinogenesis.