Mg-LDH Nanoclays Intercalated Fennel and Green Tea Active Ingredient: Field and Laboratory Evaluation of Insecticidal Activities against Culex pipiens and Their Non-Target Organisms

(1) Background: Mosquito control with essential oils is a growing demand. This work evaluated the novel larvicidal and adulticidal activity of fennel and green tea oils and their Layered double hydroxides (LDHs) nanohybrid against Culex pipiens (Cx. pipiens) in both laboratory and field conditions and evaluated their effect against non-target organisms; (2) Methods: Two types of nanoclays, MgAl-LDH and NiAl-LDH were synthesized and characterized using PXRD, TEM and SEM, whereas their elemental analysis was accomplished by SEM-EDX; (3) Results: Mg and Ni LDHs were synthesized by the co-precipitation method. The adsorption and desorption of active ingredients were conducted using LC MS/MS, with reference to the SEM-EXD analysis. The desorption process of MgAl-LDH intercalated green tea oil was conducted using ethanol, and reveled significant peaks related to polyphenols and flavonoids like Vanillin, Catechin, Daidzein, Ellagic acid, Naringenin, Myricetin and Syringic acid with concentrations of 0.76, 0.73, 0.67, 0.59, 0.52, 0.44 and 0.42 μg/g, respectively. The larvicidal LC50 values of fennel oil, Mg-LDH-F, and Ni-LDH-F were 843.88, 451.95, 550.12 ppm, respectively, whereas the corresponding values of green tea were 938.93, 530.46, and 769.94 ppm. The larval reduction percentage of fennel oil and Mg-LDH-F reached 90.1 and 96.2%, 24 h PT and their persistence reached five and seven days PT, respectively. The reduction percentage of green tea oil and Mg-LDH-GT reached 88.00 and 92.01%, 24 h PT and their persistence reached five and six days PT, respectively. Against adults, Mg-LDH-GT and Ni-LDH-GT were less effective than green tea oil as their LC95 values were 5.45, 25.90, and 35.39%, respectively. The reduction in adult density PT with fennel oil, Mg-LDH-F, green tea oil, and Mg-LDH-GT reached 83.1, 100, 77.0, and 99.0%, respectively, 24 h PT and were effective for three days. Mg-LDH-GT and Mg-LDH-F increased the predation Cybister tripunctatus (71% and 69%), respectively; (4) Conclusions: For the first time, Mg-LDH-GT and Mg-LDH-F was the best system loaded with relatively good desorption release to its active ingredients and significantly affected Cx. pipiens larvae and adults in both laboratory and field circumstances, and it could be included in mosquito control.

[1]  Abdelfattah M Selim,et al.  Larvicidal and adulticidal effects of some Egyptian oils against Culex pipiens , 2022, Scientific Reports.

[2]  Saeed Ahmed,et al.  Botanical Insecticides and their Potential as Anti-Insect/Pests: Are they Successful against Insects and Pests? , 2021, Global Decline of Insects [Working Title].

[3]  H. Ullah,et al.  Botanical Insecticides Are a Non-Toxic Alternative to Conventional Pesticides in the Control of Insects and Pests , 2021, Global Decline of Insects [Working Title].

[4]  A. Jabbar Lethal and Sub Lethal Effects of Plant Extracts and Green Silver Nanoparticles against Culex pipiens , 2021 .

[5]  Robert T. Jones,et al.  Novel control strategies for mosquito-borne diseases , 2020, Philosophical Transactions of the Royal Society B.

[6]  W. Moselhy,et al.  Efficacy of the Green Tea, Camellia sinensis Leaves Extract on Some Biological Activities of Culex pipiens and the Detection of Its Phytochemical Constituents , 2020 .

[7]  P. S. Raju,et al.  Bio-nanoparticle assembly: a potent on-site biolarvicidal agent against mosquito vectors , 2020, RSC advances.

[8]  Anchulee Pengsook,et al.  Evaluation of Alpinia galanga (Zingiberaceae) extracts and isolated trans-cinnamic acid on some mosquitoes larvae , 2019, Chemical and Biological Technologies in Agriculture.

[9]  E. Martinelli,et al.  Layered Double Hydroxides: A Toolbox for Chemistry and Biology , 2019, Crystals.

[10]  M. Abai,et al.  Comparison of CDC Bottle Bioassay with WHO Standard Method for Assessment Susceptibility Level of Malaria Vector, Anopheles stephensi to Three Imagicides , 2019, Journal of arthropod-borne diseases.

[11]  A. Selim The Recent Update of the Situation of West Nile Fever among Equids in Egypt after Three Decades of Missing Information , 2019, Pakistan Veterinary Journal.

[12]  S. Mahmoud,et al.  Toxicological activity of four plant oils against Aedes caspius and Culex pipiens (Diptera: Culicidae) , 2019 .

[13]  C. Geden,et al.  Potential of Essential Oils to Prevent Fly Strike and their Effects on the Longevity of Adult Lucilia sericata , 2018, Journal of Vector Ecology.

[14]  G. Benelli,et al.  Nanoparticles for mosquito control: Challenges and constraints , 2017 .

[15]  G. Benelli,et al.  One-pot and eco-friendly synthesis of silver nanocrystals using Adiantum raddianum: Toxicity against mosquito vectors of medical and veterinary importance , 2017 .

[16]  R. Mammadov,et al.  Phenolic composition, antioxidant, antibacterial, larvacidal against Culex pipiens, and cytotoxic activities of Hyacinthella lineata steudel extracts , 2017 .

[17]  H. Mo,et al.  Therapeutic properties of green tea against environmental insults. , 2017, The Journal of nutritional biochemistry.

[18]  Rowida Baeshen,et al.  Botanical insecticide as simple extractives for pest control , 2017 .

[19]  Joel L. Bargul,et al.  Potential of Camellia sinensis proanthocyanidins-rich fraction for controlling malaria mosquito populations through disruption of larval development , 2016, Parasites & Vectors.

[20]  G. Benelli,et al.  Single-step biosynthesis and characterization of silver nanoparticles using Zornia diphylla leaves: A potent eco-friendly tool against malaria and arbovirus vectors. , 2016, Journal of photochemistry and photobiology. B, Biology.

[21]  G. Benelli,et al.  One-pot fabrication of silver nanocrystals using Nicandra physalodes: A novel route for mosquito vector control with moderate toxicity on non-target water bugs. , 2016, Research in veterinary science.

[22]  A. Ashafa,et al.  Larvicidal, pupicidal and insecticidal activities of Cosmos bipinnatus , Foeniculum vulgare and Tagetes minuta against Culex quinquefasciatus mosquitoes , 2016 .

[23]  J. Tříska,et al.  New knowledge for yield, composition and insecticidal activity of essential oils obtained from the aerial parts or seeds of fennel (Foeniculum vulgare Mill.) , 2016 .

[24]  J. M. Rubio,et al.  MOSQUITO IDENTIFICATION AND MOLECULAR XENOMONITORING OF LYMPHATIC FILARIASIS IN SELECTED ENDEMIC AREAS IN GIZA AND QUALIOUBIYA GOVERNORATES, EGYPT. , 2016, Journal of the Egyptian Society of Parasitology.

[25]  R. Ogali,et al.  The Role of Plant Essential Oils in Mosquito (Diptera: Culicidae) Control , 2016 .

[26]  G. Benelli,et al.  Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito vectors , 2015, Parasitology Research.

[27]  A. Higuchi,et al.  Characterization and biotoxicity of Hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against Aedes aegypti and Plutella xylostella. , 2015, Ecotoxicology and environmental safety.

[28]  R. Messing,et al.  Predation by Asian bullfrog tadpoles, Hoplobatrachus tigerinus, against the dengue vector, Aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles , 2015, Parasitology Research.

[29]  C. Moiteiro,et al.  Larvicidal Activity against Aedes Aegypti of Foeniculum Vulgare Essential Oils from Portugal and Cape Verde , 2015, Natural product communications.

[30]  M. T. Novo,et al.  Larvicidal, molluscicidal and nematicidal activities of essential oils and compounds from Foeniculum vulgare , 2014, Journal of Pest Science.

[31]  S. Hay,et al.  The global distribution and transmission limits of lymphatic filariasis: past and present , 2014, Parasites & Vectors.

[32]  A. Baâliouamer,et al.  Chemical composition and larvicidal activity of Algerian Foeniculum vulgare seed essential oil , 2014 .

[33]  P. U. Rani,et al.  Biofabrication of Ag nanoparticles using Sterculia foetida L. seed extract and their toxic potential against mosquito vectors and HeLa cancer cells. , 2014, Materials science & engineering. C, Materials for biological applications.

[34]  H. Khater Bioactivity of Essential Oils as Green Biopesticides : Recent Global Scenario , 2013 .

[35]  P. Vasiljević,et al.  Larvicidal activity and in vitro effects of green tea (Camellia sinensis L.) water infusion , 2013 .

[36]  H. Khater PROSPECTS OF BOTANICAL BIOPESTICIDES IN INSECT PEST MANAGEMENT , 2012 .

[37]  C. Patil,et al.  Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and nontarget fish Poecillia reticulata , 2012, Parasitology Research.

[38]  R. Barbehenn,et al.  Tannins in plant-herbivore interactions. , 2011, Phytochemistry.

[39]  A. Jitpakdi,et al.  Chemical Composition and Larvicidal Activity of Edible Plant-Derived Essential Oils Against the Pyrethroid-Susceptible and -Resistant Strains of Aedes aegypti (Diptera: Culicidae) , 2010, Journal of vector ecology : journal of the Society for Vector Ecology.

[40]  I. Cho,et al.  Insecticidal Activity of Basil Oil, trans-Anethole, Estragole, and Linalool to Adult Fruit Flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae , 2009, Journal of economic entomology.

[41]  S. Mayadevi,et al.  Adsorption of fluoride ions by Zn–Al layered double hydroxides , 2008 .

[42]  H. Khater,et al.  Potential of biologically active plant oils to control mosquito larvae (Culex pipiens, Diptera: Culicidae) from an Egyptian locality. , 2008, Revista do Instituto de Medicina Tropical de Sao Paulo.

[43]  P. Kamath,et al.  Chromate uptake characteristics of the pristine layered double hydroxides of Mg with Al , 2008 .

[44]  K. Kandori,et al.  Anion-exchange and thermal change of layered zinc hydroxides formed in the presence of Al(III) , 2007 .

[45]  Hongtao Wang,et al.  Defluoridation of drinking water by Mg/Al hydrotalcite-like compounds and their calcined products , 2007 .

[46]  K. Parida,et al.  Preparation and characterization of Mg-Al hydrotalcite-like compounds containing cerium. , 2006, Journal of colloid and interface science.

[47]  X. Duan,et al.  Applications of Layered Double Hydroxides , 2006 .

[48]  A. Jitpakdi,et al.  Essential oils as potential adulticides against two populations of Aedes aegypti, the laboratory and natural field strains, in Chiang Mai province, northern Thailand , 2006, Parasitology Research.

[49]  Z. Hao,et al.  Adsorption/desorption studies of NOx on well-mixed oxides derived from Co-Mg/Al Hydrotalcite-like compounds. , 2006, The journal of physical chemistry. B.

[50]  X. Duan,et al.  Preparation of layered double hydroxides and their applications as additives in polymers, as precursors to magnetic materials and in biology and medicine. , 2006, Chemical communications.

[51]  A. Jitpakdi,et al.  Effectiveness of Zanthoxylum piperitum-derived essential oil as an alternative repellent under laboratory and field applications , 2006, Parasitology Research.

[52]  E. López-Salinas,et al.  SOx removal by calcined MgAlFe hydrotalcite-like materials: effect of the chemical composition and the cerium incorporation method. , 2005, Environmental science & technology.

[53]  Carlos A. Grande,et al.  Hydrotalcite Materials for Carbon Dioxide Adsorption at High Temperatures: Characterization and Diffusivity Measurements , 2005 .

[54]  A. Ćwik,et al.  An efficient and environmental-friendly synthesis of 4-hydroxy-arylpiperidines using hydrotalcite catalyst , 2004 .

[55]  S. Srivastava,et al.  Adsorption of Cr(VI) and Se(IV) from their aqueous solutions onto Zr4+-substituted ZnAl/MgAl-layered double hydroxides: effect of Zr4+ substitution in the layer. , 2004, Journal of colloid and interface science.

[56]  B. Dousova,et al.  Sorption of As(V) Species from Aqueous Systems , 2003 .

[57]  D. D. De Vos,et al.  Hydrotalcite-like anionic clays in catalytic organic reactions , 2001 .

[58]  C. S. Yang,et al.  Effects of tea consumption on nutrition and health. , 2000, The Journal of nutrition.

[59]  J. W. Boclair,et al.  Layered double hydroxide stability. 1. Relative stabilities of layered double hydroxides and their simple counterparts. , 1999, Chemistry of materials : a publication of the American Chemical Society.

[60]  M. Kantam,et al.  Henry reactions catalysed by modified Mg–Al hydrotalcite:. an efficient reusable solid base for selective synthesis of β-nitroalkanols† , 1999 .

[61]  Y. Tamaura,et al.  Synthesis of hydrotalcite with high layer charge for CO2 adsorbent , 1995 .

[62]  Robert J. Davis,et al.  Investigation of the surface structure and basic properties of calcined hydrotalcites , 1992 .

[63]  H. Schaper,et al.  Stabilized magnesia: A novel catalyst (support) material , 1989 .

[64]  M. Mulla,et al.  Control of Chironomid Midges in Recreational Lakes , 1971 .