A New Method for PCR Reaction from Alkali-Treated Rice Leaf Tissues

A new method for PCR reaction from a small quantity of rice leaf tissue with alkali-treated was presented, which can be applied either to identify genotype of rice variety and its purity, or to assist selection for molecular breeding. This method is rapid, simple and reliable, and only needs a small part of rice leaf tissue that does not damage the whole plant. It does not need high speed refrigerated centrifuge and the reagents for DNA extraction. Especially, it is more economic and effective for a large-scale PCR tests. Normally 300 samples can be processed within a day by this method.