Production of Biocellulosic Ethanol from Wheat Straw

Wheat straw is an abundant lignocellulosic feedstock in many parts of the world, and has been selected for producing ethanol in an economically feasible manner. It contains a mixture of sugars (hexoses and pentoses).Two-stage acid hydrolysis was carried out with concentrates of perchloric acid, using wheat straw. The hydrolysate was concentrated by vacuum evaporation to increase the concentration of fermentable sugars, and was detoxified by over-liming to decrease the concentration of fermentation inhibitors. After two-stage acid hydrolysis, the sugars and the inhibitors were measured. The ethanol yields obtained from by converting hexoses and pentoses in the hydrolysate with the co-culture of Saccharomyces cerevisiae and Pichia stipites were higher than the ethanol yields produced with a monoculture of S. cerevisiae . Various conditions for hysdrolysis and fermentation were investigated. The ethanol concentration was 11.42 g/l in 42 h of incubation, with a yield of 0.475 g/g, productivity of 0.272 gl ·h, and fermentation efficiency of 92.955 %, using a co-culture of Saccharomyces cerevisiae and Pichia stipites

[1]  Anne Belinda Thomsen,et al.  Production of ethanol from wet oxidised wheat straw by Thermoanaerobacter mathranii , 1999 .

[2]  L. Rao,et al.  Bioethanol fermentation of concentrated rice straw hydrolysate using co-culture of Saccharomyces cerevisiae and Pichia stipitis. , 2011, Bioresource technology.

[3]  B. Simmons,et al.  Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification. , 2010, Bioresource technology.

[4]  Tomas Brandberg,et al.  Comparison of vacuum and high pressure evaporated wood hydrolyzate for enthanol production by repeated fed-batch using flocculating Saccharomyces cerevisiae , 2009, BioResources.

[5]  Mohammad J. Taherzadeh,et al.  Effect of pH, time and temperature of overliming on detoxification of dilute-acid hydrolyzates for fermentation by Saccharomyces cerevisiae , 2002 .

[6]  K. Jensen,et al.  Pretreatment of wheat straw and conversion of xylose and xylan to ethanol by thermophilic anaerobic bacteria , 1996 .

[7]  L. Olsson,et al.  Potential inhibitors from wet oxidation of wheat straw and their effect on ethanol production of Saccharomyces cerevisiae: wet oxidation and fermentation by yeast. , 2003, Biotechnology and bioengineering.

[8]  K. T. Semrau,et al.  Wood hydrolysis for ethanol production — previous experience and the economics of selected processes , 1984 .

[9]  Bärbel Hahn-Hägerdal,et al.  Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. , 2000 .

[10]  A. Baker.  TWO-STAGE, DILUTE SULFURIC ACID HYDROLYSIS OF HARDWOOD FOR ETHANOL PRODUCTION , 2007 .

[11]  J. Gould.  Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification , 1984, Biotechnology and bioengineering.

[12]  J. Delgenès,et al.  Acid hydrolysis of wheat straw and process considerations for ethanol fermentation by Pichia stipitis Y7124 , 1990 .

[13]  M. Taherzadeh,et al.  Acid-based hydrolysis processes for ethanol from lignocellulosic materials: A review , 2007, BioResources.

[14]  Mark T. Holtzapple,et al.  The ammonia freeze explosion (AFEX) process , 1991 .

[15]  Sunny E. Iyuke,et al.  Temperature optimization for bioethanol production from corn cobs. , 2010 .

[16]  B. Prior,et al.  A quantitative screening of some xylose-fermenting yeast isolates , 1985, Biotechnology Letters.

[17]  C. Kurtzman,et al.  Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. , 1981, Biotechnology and bioengineering.

[18]  F. Carvalheiro,et al.  Dilute Acid Hydrolysis of Wheat Straw Oligosaccharides , 2009, Applied biochemistry and biotechnology.

[19]  Gary D. McGinnis,et al.  Biomass pretreatment with water and high-pressure oxygen. The wet-oxidation process , 1983 .

[20]  C. Biermann,et al.  Steam explosion of mixed hardwood chips, rice hulls, corn stalks, and sugar cane bagasse , 1984 .

[21]  Luís C. Duarte,et al.  Hemicellulose biorefineries: a review on biomass pretreatments , 2008 .

[22]  A. Bjerre,et al.  Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose , 2000, Biotechnology and bioengineering.

[23]  J N Nigam,et al.  Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis. , 2001, Journal of biotechnology.

[24]  E. Chornet,et al.  Application of steam explosion to the fractionation and rapid vapor-phase alkaline pulping of wheat straw , 1998 .

[25]  Anne Belinda Thomsen,et al.  Comparison between wet oxidation and steam explosion as pretreatment methods for enyzmatic hydrolysis of sugarcane bagasse , 2008, BioResources.