Electroporation chip for adherent cells on photochemically modified polymer surfaces

We present a polytetrafluoroethylene electroporation microchip with integrated electrodes for transfection of adherent biological cells. For fabrication, UV-surface modification was employed in combination with metal deposition. UV irradiation in reactive atmosphere resulted in introduction of polar chemical groups into the polytetrafluoroethylene surface for significant adhesion enhancement of both biological cells as well as metal electrodes thereon. Electroporation was demonstrated by transfection of human embryonic kidney cells with the enhanced green fluorescent protein. Transparent, working at low voltages, and easy to handle, this chip yields the potential to reduce the amount of sequential working steps necessary for transfection.

[1]  S. Bacchetti,et al.  Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  Dieter Baeuerle,et al.  Laser-enhanced adhesion and thin film formation , 1996, Other Conferences.

[3]  R. Meldrum,et al.  Optimisation of electroporation for biochemical experiments in live cells. , 1999, Biochemical and biophysical research communications.

[4]  Steve W. Smye,et al.  Membrane electroporation theories: a review , 2006, Medical and Biological Engineering and Computing.

[5]  J. Weaver,et al.  Theory of electroporation: A review , 1996 .

[6]  Hiroo Iwata,et al.  Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes. , 2004, Nucleic acids research.

[7]  E Neumann,et al.  Fundamentals of electroporative delivery of drugs and genes. , 1999, Bioelectrochemistry and bioenergetics.

[8]  J. Northrop,et al.  Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[9]  Dieter Bäuerle,et al.  Cell proliferation on UV-excimer lamp modified and grafted polytetrafluoroethylene , 2004 .

[10]  U. Zimmermann,et al.  Measurement of the permeability and resealing time constant of the electroporated mammalian cell membranes , 2004 .

[11]  Yu-Cheng Lin,et al.  Enhancement of an electroporation system for gene delivery using electrophoresis with a planar electrode. , 2007, Lab on a chip.

[12]  K. Kelnar,et al.  High-throughput RNAi screening in vitro: from cell lines to primary cells. , 2005, RNA.

[13]  Yu-Cheng Lin,et al.  Electroporation microchips for in vitro gene transfection , 2001 .

[14]  E. Neumann,et al.  Permeability changes induced by electric impulses in vesicular membranes , 1972, The Journal of Membrane Biology.

[15]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[16]  T. Reese,et al.  Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy. , 1990, Biophysical journal.

[17]  T. Tsong,et al.  Study of mechanisms of electric field-induced DNA transfection. I. DNA entry by surface binding and diffusion through membrane pores. , 1990, Biophysical journal.

[18]  Michael Olbrich,et al.  Simple and versatile methods for the fabrication of arrays of live mammalian cells. , 2006, Lab on a chip.

[19]  Michael Olbrich,et al.  Cell microarrays on photochemically modified polytetrafluoroethylene. , 2005, Biomaterials.

[20]  K. Kent,et al.  Microinjection of DNA into the nuclei of human vascular smooth muscle cells. , 2002, The Journal of surgical research.

[21]  Yu-Cheng Lin,et al.  Electroporation microchips for continuous gene transfection , 2001 .