Effective photocatalytic disinfection of E. coli and S. aureus using polythiophene/MnO2 nanocomposite photocatalyst under solar light irradiation

Abstract The photocatalytic disinfection of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated systematically with polythiophene/MnO2 (PTh/MnO2) nanocomposite photocatalyst under solar light irradiation. The prepared PTh/MnO2 nanocomposite was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectrum (FT-IR) and ultraviolet and visible absorption spectrum (UV-vis). The influence parameters of disinfection by PTh/MnO2 nanocomposite, such as irradiation time and concentration of photocatalyst were studied. Further investigation indicated that almost all (99.9%) of the initial bacteria were killed after 6 h incubation at 37 °C in the presence of 1 mg/mL PTh/MnO2 photocatalyst under solar light irradiation. During the photocatalytic process, the hydroxyl radical (⋅OH) was generated under irradiation, which played a key role in the inactivation of bacteria. The morphological change of the bacteria during the bactericidal process could be observed from TEM images. This work provides a potential effective nanocomposite photocatalyst to disinfect E. coli and S. aureus under solar light irradiation.

[1]  Chuncheng Chen,et al.  Visible-light-induced aerobic oxidation of alcohols in a coupled photocatalytic system of dye-sensitized TiO2 and TEMPO. , 2008, Angewandte Chemie.

[2]  G. Song,et al.  Photocatalytic degradation of organic pollutants and detection of chemical oxygen demand by fluorescence methods , 2009 .

[3]  J. Qu,et al.  Ag/AgBr/TiO2 visible light photocatalyst for destruction of azodyes and bacteria. , 2006, The journal of physical chemistry. B.

[4]  Z. Zainal,et al.  Bactericidal Activity of TiO2 Photocatalyst in Aqueous Media: Toward a Solar-Assisted Water Disinfection System. , 1994, Environmental science & technology.

[5]  W. Bentley,et al.  Toxicogenomic response of Staphylococcus aureus to peracetic acid. , 2006, Environmental Science and Technology.

[6]  T. Nakajima,et al.  Photoelectrochemical sterilization of microbial cells by semiconductor powders , 1985 .

[7]  X. Lou,et al.  Porous Spheres Assembled from Polythiophene (PTh)-Coated Ultrathin MnO2 Nanosheets with Enhanced Lithium Storage Capabilities , 2010 .

[8]  Jiang Chang,et al.  Photocatalytic inactivation of bacteria by photocatalyst Bi2WO6 under visible light , 2009 .

[9]  Qing Lu,et al.  Synthesis of mesoporous polythiophene/MnO 2 nanocomposite and its enhanced pseudocapacitive properti , 2011 .

[10]  Darlene K. Slattery,et al.  Photocatalytic Inhibition of Algae Growth Using TiO2, WO3, and Cocatalyst Modifications , 2000 .

[11]  Yunfeng Zhu,et al.  Photocatalytic degradation of methyl orange using polythiophene/titanium dioxide composites , 2010 .

[12]  R. Asahi,et al.  Visible-Light Photocatalysis in Nitrogen-Doped Titanium Oxides , 2001, Science.

[13]  T. Pan,et al.  Immunomodulating activity of Lactobacillus paracasei subsp. paracasei NTU 101 in enterohemorrhagic Escherichia coli O157H7-infected mice. , 2010, Journal of agricultural and food chemistry.

[14]  J. Qu,et al.  Photocatalytic degradation of pathogenic bacteria with AgI/TiO2 under visible light irradiation. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[15]  Q. Ma,et al.  Facile preparation of PbO2 electrode for the electrochemical inactivation of microorganisms , 2009 .

[16]  F. Šeršeň,et al.  Inactivation of bacteria G(+)-S. aureus and G(-)-E. coli by phototoxic polythiophene incorporated in ZSM-5 zeolite. , 2006, Chemosphere.

[17]  R. W. Fessenden,et al.  Rate Constants for Charge Injection from Excited Sensitizer into SnO2, ZnO, and TiO2 Semiconductor Nanocrystallites , 1995 .

[18]  R. Jothiramalingam,et al.  Synthesis, characterization and photocatalytic activity of porous manganese oxide doped titania for toluene decomposition. , 2007, Journal of hazardous materials.

[19]  P. He,et al.  A Polyaniline‐Intercalated Layered Manganese Oxide Nanocomposite Prepared by an Inorganic/Organic Interface Reaction and Its High Electrochemical Performance for Li Storage , 2008 .

[20]  J. Qu,et al.  Efficient destruction of pathogenic bacteria with AgBr/TiO2 under visible light irradiation , 2007 .

[21]  M. Bekbolet.  Photocatalytic bactericidal activity of TiO2 in aqueous suspensions of E. coli , 1997 .

[22]  Akira Fujishima,et al.  Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts , 1998 .

[23]  Xin-Gui Li,et al.  Facile optimal synthesis of inherently electroconductive polythiophene nanoparticles. , 2009, Chemistry.

[24]  Zidong Wang,et al.  Electrodeposited PbO2 thin film as positive electrode in PbO2/AC hybrid capacitor , 2009 .

[25]  M. Endo,et al.  The preparation of multi-walled carbon nanotubes with a Ni–P coating by an electroless deposition process , 2005 .

[26]  K. T. Kawagoe,et al.  Electrocatalysis of Anodic Oxygen‐Transfer Reactions Oxidation of Phenol and Benzene at Bismuth‐Doped Lead Dioxide Electrodes in Acidic Solutions , 1994 .

[27]  Xiaoyan Qin,et al.  Ag/AgBr/WO(3).H(2)O: visible-light photocatalyst for bacteria destruction. , 2009, Inorganic chemistry.

[28]  Omid Akhavan,et al.  Photocatalytic Reduction of Graphene Oxide Nanosheets on TiO2 Thin Film for Photoinactivation of Bacteria in Solar Light Irradiation , 2009 .

[29]  R. Vijayaraghavan,et al.  Photocatalytic inactivation of spores of Bacillus anthracis using titania nanomaterials. , 2011, Journal of hazardous materials.

[30]  M. Gholami,et al.  Apatite-coated Ag/AgBr/TiO(2) visible-light photocatalyst for destruction of bacteria. , 2007, Journal of the American Chemical Society.

[31]  Jiaguo Yu,et al.  Efficient visible-light-induced photocatalytic disinfection on sulfur-doped nanocrystalline titania. , 2005, Environmental science & technology.

[32]  Long Jiang,et al.  Visible Light Induced Photocatalytic Degradation of Methyl Orange by Polythiophene/TiO2 Composite Particles , 2010 .

[33]  Lisha Zhang,et al.  Effective photocatalytic disinfection of E. coli K-12 using AgBr-Ag-Bi2WO6 nanojunction system irradiated by visible light: the role of diffusing hydroxyl radicals. , 2010, Environmental science & technology.

[34]  J. Qu,et al.  Efficient destruction of pathogenic bacteria with NiO/SrBi2O4 under visible light irradiation. , 2006, Environmental science & technology.

[35]  F. Wudl,et al.  Solid-state synthesis of a conducting polythiophene via an unprecedented heterocyclic coupling reaction. , 2003, Journal of the American Chemical Society.