Localized in vivo lH NMR detection of neurotransmitter labeling in rat brain during infusion of [ 1-13 C ] D-glucose

Introduction: In vivo 13C NMR spectroscopy with localization is emerging as an important tool to study intermediary metabolism non-invasively in the brain [ 11. One major drawback of ‘H-detected 13C labeling in vivo has been the difficulty of detecting 13C label simultaneously in several metabolites [2]. The purpose of this study was to investigate whether, in ‘H spectra, the labeling of glutamine and other metabolites can be simultaneously observed with that of neuronal glutamate so as to permit investigation of glial-neuronal cycling of neurotransmitter glutamate [3]. An ‘Adiabatic Carbon Editing and Decoupling’ sequence (ACED), based on ‘H STEAM localization, is introduced that is easy to implement and 13C broadband editing is achieved using adiabatic pulses requiring low peak RF power.