2‐Aminoethoxydiphenyl borate (2‐APB) is a reliable blocker of store‐operated Ca2+ entry but an inconsistent inhibitor of InsP3‐induced Ca2+ release

Since its introduction to Ca2+ signaling in 1997, 2‐aminoethoxydiphenyl borate (2‐APB) has been used in many studies to probe for the involvement of inositol 1,4,5‐trisphosphate receptors in the generation of Ca2+ signals. Due to reports of some nonspecific actions of 2‐APB, and the fact that its principal antagonistic effect is on Ca2+ entry rather than Ca2+ release, this compound may not have the utility first suggested. However, 2‐APB has thrown up some interesting results, particularly with respect to store‐operated Ca2+ entry in nonexcitable cells. These data indicate that although it must be used with caution, 2‐APB can be useful in probing certain aspects of Ca2+ signaling.—Bootman, M. D., Collins, T. J., Mackenzie, L., Roderick, H. L., Berridge, M. J., Peppiatt, C. M. 2‐Aminoethoxydiphenyl borate (2‐APB) is a reliable blocker of store‐operated Ca2+ entry but an inconsistent inhibitor of InsP3‐induced Ca2+ release. FASEB J. 16, 1145–1150 (2002)

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