A microcomputer program for fitting enzyme inhibition rate equations.

This paper presents a computer program written in BASIC for fitting different enzyme inhibition kinetic models. The program, based on a nonlinear least-squares regression, can be run on any microcomputer with the CP/M operating system. Weighting of observed initial velocities is decided by the user by assessing constant variance, proportional variance or by incorporation of the variances calculated by a subroutine. The program also uses robust regression by bisquare weighting. All questions concerning data input, type of rate function, type of weight and the use of bisquare regression appear on the video display unit.

[1]  A. Cornish-Bowden,et al.  Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelai , 1974, The Biochemical journal.

[2]  R G Duggleby,et al.  Regression analysis of nonlinear Arrhenius plots: an empirical model and a computer program. , 1984, Computers in biology and medicine.

[3]  R G Duggleby,et al.  A nonlinear regression program for small computers. , 1981, Analytical biochemistry.

[4]  W. Cleland,et al.  Statistical analysis of enzyme kinetic data. , 2006, Methods in enzymology.

[5]  D Garfinkel,et al.  A computer program for analyzing enzyme kinetic data using graphical display and statistical analysis. , 1984, Computers and biomedical research, an international journal.

[6]  G F Pinto,et al.  Pocket computer program for fitting the Hill equation. , 1984, Computers in biology and medicine.

[7]  E. Havir,et al.  A computer program for fitting data to the Michaelis-Menten equation. , 1967, Biochemical and biophysical research communications.

[8]  D Garfinkel,et al.  A convenient computer program for fitting enzymatic rate laws to steady-state data. , 1979, Computers and biomedical research, an international journal.

[9]  M J Crabbe,et al.  An enzyme kinetics program for desk-top computers. , 1982, Computers in biology and medicine.

[10]  G. Atkins,et al.  A simple digital-computer program for estimating the parameters of the hill equation. , 1973, European journal of biochemistry.

[11]  H J Fromm,et al.  A computer program for fitting and statistically analyzing initial rate data applied to bovine hexokinase type III isozyme. , 1975, Archives of biochemistry and biophysics.

[12]  W. Cleland,et al.  Enzyme kinetics revisited: a commentary on 'The Kinetics of Enzyme-Catalyzed Reactions With Two or More Substrates or Products'. , 1989, Biochimica et biophysica acta.

[13]  M. Crabbe.  Distribution-free computer methods for analysing ligand binding and enzyme mechanisms. , 1985, Computers in biology and medicine.

[14]  G. Atkins,et al.  Methods for fitting equations with two or more non-linear parameters. , 1976, Biochemical Journal.

[15]  M J Crabbe,et al.  Microcomputer analysis of hyperbolic and non-hyperbolic steady-state kinetics. , 1984, International journal of bio-medical computing.

[16]  W. Cleland,et al.  The statistical analysis of enzyme kinetic data. , 1967, Advances in enzymology and related areas of molecular biology.

[17]  G L Atkins,et al.  Current trends in the estimation of Michaelis-Menten parameters. , 1980, Analytical biochemistry.

[18]  G F Pinto,et al.  Pocket computer program for fitting the Michaelis-Menten equation. , 1983, Computers in biology and medicine.

[19]  W Ferdinand,et al.  The interpretation of non-hyperbolic rate curves for two-substrate enzymes. A possible mechanism for phosphofructokinase. , 1966, The Biochemical journal.

[20]  W R Greco,et al.  ROSFIT: an enzyme kinetics nonlinear regression curve fitting package for a microcomputer. , 1982, Computers and biomedical research, an international journal.

[21]  W. Cleland,et al.  The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory. , 1963, Biochimica et biophysica acta.