Parameters affecting cellular adhesion to polylactide films.

Absorbable biomaterials have been recently incorporated into the field of tissue engineering. Little work has been performed, even with the clinically acceptable absorbables, concerning their tissue promoting capability or lack, thereof. Furthermore, the relative attractions of cells to these implants may be largely disguised by the presence of serum. This research involved the development of an adhesion assay to compare the adhesion behavior of two cell types to two different polylactides in a serum free environment. The results showed that the attachment behavior depends not only on the cell or the polymer but a combination of the two.

[1]  D E Ingber,et al.  Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix , 1989, The Journal of cell biology.

[2]  R Langer,et al.  Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. , 1993, Journal of biomedical materials research.

[3]  E. Ruoslahti,et al.  Extracellular matrices and cell adhesion. , 1985, Arteriosclerosis.

[4]  V. Quaranta,et al.  Coating of titanium alloy with soluble laminin-5 promotes cell attachment and hemidesmosome assembly in gingival epithelial cells: potential application to dental implants. , 1997, Journal of periodontal research.

[5]  D E Ingber,et al.  Hepatocyte culture on biodegradable polymeric substrates , 1991, Biotechnology and bioengineering.

[6]  David J. Mooney,et al.  Increased Vascularization and Heterogeneity of Vascular Structures Occurring in Polyglycolide Matrices Containing Aortic Endothelial Cells Implanted in the Rat , 1997 .

[7]  S. Nagahara,et al.  Cell-substrate and cell-cell interactions differently regulate cytoskeletal and extracellular matrix protein gene expression. , 1996, Journal of biomedical materials research.

[8]  C. Streuli,et al.  Control of Integrin Expression by Extracellular Matrix (*) , 1995, The Journal of Biological Chemistry.

[9]  S. Shalaby,et al.  Change in stiffness and effect of orientation in degrading polylactide films. , 1998, Biomaterials.

[10]  K. J. Long,et al.  Surface roughness, porosity, and texture as modifiers of cellular adhesion. , 1996, Tissue engineering.

[11]  D E Ingber,et al.  Fibronectin controls capillary endothelial cell growth by modulating cell shape. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D E Ingber,et al.  Cellular tensegrity: exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis. , 1994, International review of cytology.

[13]  J. Brugge,et al.  Integrins and signal transduction pathways: the road taken. , 1995, Science.

[14]  D. Ingber,et al.  Prevascularization of porous biodegradable polymers , 1993, Biotechnology and bioengineering.