Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Two lymphoid cell lines were established from a patient with chronic lymphocytic leukemia by infecting blood cells with Epstein‐Barr virus (EBV). Studies of morphology, glucose‐6‐phosphate dehydrogenase, malic enzyme, immunoglobulin, and chromosomes of the two lines indicated that one of them originated from leukemic cells while the other arose from residual normal blood cells. The morphology and capacity for immunoglobulin secretion in the line that arose from leukemic cells were similar to those found in EVB‐carrying lymphoblastoid cell lines grown from patients without neoplasia and differed from those seen in fresh chronic lymphocytic leukemia cells. These observations suggest that the introduction of EBV into the leukemic cells may have caused them to differentiate in a fashion similar to that noted in normal B cells after exposure to EBV.

[1]  G. Klein,et al.  Chromosome analyses of lymphoid cell lines derived from patients with chronic lymphocytic leukemia , 1980, International journal of cancer.

[2]  L. Terenius,et al.  Biochemical characteristics of chronic lymphocytic leukaemia. Glucocorticoid receptors, B and T lymphocyte surface markers, concanavalin A-induced agglutination and thymidine incorporation. , 2009, Scandinavian journal of haematology.

[3]  H. Kunkel,et al.  Lymphoblastoid cell lines from patients with chronic lymphocytic leukemia: identification of tumor origin by idiotypic analysis. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Barker,et al.  Genetic polymorphism of human phosphoglycolate phosphatase (PGP) , 1978, Annals of human genetics.

[5]  V. Najfeld,et al.  CHRONIC LYMPHOCYTIC LEUKÆMIA: CLONAL ORIGIN IN A COMMITTED B-LYMPHOCYTE PROGENITOR , 1978, The Lancet.

[6]  A. Saxon,et al.  Immunologic characterization of hairy cell leukemias in continuous culture. , 1978, Journal of immunology.

[7]  R. Good,et al.  Rosette formation with mouse erythrocytes. IV. T, B and third population cells in human tonsils. , 1977, Clinical and experimental immunology.

[8]  S. Povey,et al.  Characterisation of human cell lines and differentiation from HeLa by enzyme typing , 1976, Nature.

[9]  C. Huber,et al.  A simple rosette assay for demonstration of complement receptor sites using complement‐coated zymosan beads , 1975, European journal of immunology.

[10]  H. Harris,et al.  Handbook of enzyme electrophoresis in human genetics , 1976 .

[11]  S. Povey,et al.  Sub‐unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human‐mouse hybrids , 1975, Annals of human genetics.

[12]  J. Pontén,et al.  Classification and biological nature of established human hematopoietic cell lines , 1975, International journal of cancer.

[13]  G. Klein,et al.  Immunoglobulin synthesis and glucose-6-phosphate dehydrogenase as cell markers in human lymphoblastoid cell lines. , 1974, Experimental cell research.

[14]  S. Douglas,et al.  Biochemical and morphological observations on lymphocytes in continuous cell culture from a patient with chronic lymphocytic leukemia. , 1974, Acta haematologica.

[15]  Z. Bentwich,et al.  SHEEP RED CELL BINDING TO HUMAN LYMPHOCYTES TREATED WITH NEURAMINIDASE; ENHANCEMENT OF T CELL BINDING AND IDENTIFICATION OF A SUBPOPULATION OF B CELLS , 1973, The Journal of experimental medicine.

[16]  G. Klein,et al.  Cellular localization of an Epstein‐Barr virus (EBV)‐associated complement‐fixing antigen in producer and non‐producer lymphoblastoid cell lines , 1973, International journal of cancer.

[17]  B. Andersson,et al.  Evidence for a Receptor Recognizing Antigen Complexed Immunoglobulin on the Surface of Activated Mouse Thymus Lymphocytes , 1972, Scandinavian journal of immunology.

[18]  H. Wigzell,et al.  SURFACE MARKERS ON HUMAN T AND B LYMPHOCYTES , 1972, The Journal of experimental medicine.

[19]  K. Nilsson.  High‐frequency establishment of human immunoglobulin‐producing lymphoblastoid lines from normal and malignant lymphoid tissue and peripheral blood , 1971, International journal of cancer.

[20]  Townsend De,et al.  Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase. , 1969 .

[21]  S. Gartler,et al.  Clonal origin of chronic myelocytic leukemia in man. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C. W. Parr,et al.  Human Red-cell Phosphogluconate Dehydrogenases , 1963, Nature.

[23]  W. Lóffler.  Disorders of the chest. , 1947, Lancet.