论文信息 - On the validity of Rayleigh–Gans–Debye type approximations in red blood cell size determination

On the validity of Rayleigh–Gans–Debye type approximations in red blood cell size determination

The validity of the Rayleigh-Gans-Debye (RGD) approximation for the size determination of a soft prolate spheroidal scatterer has been examined. Calculations have been carried out for the volume range 30–100 µm3 and for aspect ratios ranging from 1.1 to 4.0. Particular attention has been paid to size determination of a red blood cell (RBC) in a flow cytometric arrangement. The applicability of the RGD approximation has been compared with its two modified forms and also with the WKB approximation.

Manoj K. Yadav | M. Yadav | Subodh K. Sharma

[1] P. Barber,et al. Rayleigh-Gans-Debye applicability to scattering by nonspherical particles. , 1978, Applied optics.

[2] A. Patitsas. A simple method for determining the size of a sphere from the extrema of the scattering intensity. I. Dielectric sphere , 1973 .

[3] A A Doroshkin,et al. Measurement of scattering properties of individual particles with a scanning flow cytometer. , 1995, Applied optics.

[4] J. Ravey. The first extrema in the radiation pattern of the light scattered by micrometer-sized spheres and spheroids , 1985 .

[5] Absolute real-time measurement of particle size distribution with the flying light-scattering indicatrix method. , 1996, Applied optics.

[6] D. J. Somerford,et al. NEW APPROXIMATE PHASE FUNCTIONS FOR SCATTERING OF UNPOLARIZED LIGHT BY DIELECTRIC PARTICLES , 1998 .

[7] Tuan-Wu Chen. Diffraction by a spherical dielectric at large size parameter , 1994 .

[8] C. Tropea,et al. Light Scattering from Small Particles , 2003 .

[9] D. Saxon. Lectures on the scattering of light , 1974 .

[10] Koichi Shimizu. Modification of the Rayleigh–Debye approximation , 1983 .

[11] D. Somerford,et al. Modified Rayleigh-Gans-Debye approximations applied to sizing transparent homogeneous long fibres of intermediate size , 1988 .

[12] S. Sharma,et al. Light Scattering by Optically Soft Particles: Theory and Applications , 2006 .