Metabolism of intracellular polysaccharide in the cells of Streptococcus mutans under strictly anaerobic conditions.

Streptococcus mutans, which had accumulated glycogen-like iodophilic intracellular polysaccharide (IPS), produced large amounts of formate, acetate and ethanol from the IPS by pyruvate formate-lyase (PFL) under strictly anaerobic conditions without exogenous sugar. Under aerobic conditions, the same S. mutans produced exclusively lactate and pyruvate from the IPS because of the inactivation of PFL by oxygen. The total amount of acid produced under anaerobic conditions was larger than that under aerobic conditions. The analysis of intracellular glycolytic intermediates revealed that levels of fructose 1,6-bisphosphate (lactate dehydrogenase (LDH) activator) and glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (PFL inhibitors) were low when IPS was used as a glycolytic substrate, implying that PFL functions more efficiently than LDH in IPS metabolism. These findings suggest that the PFL pathway contributes to the acid production from IPS, and may explain partially why the acids in starved dental plaque are mainly acetate and formate.

[1]  N. Takahashi,et al.  Oxygen sensitivity of sugar metabolism and interconversion of pyruvate formate-lyase in intact cells of Streptococcus mutans and Streptococcus sanguis , 1987, Infection and immunity.

[2]  W. Distler,et al.  Formic acid in human single-site resting plaque--quantitative and qualitative aspects. , 1986, Caries research.

[3]  J. Carlsson,et al.  Pyruvate dehydrogenase activity in Streptococcus mutans , 1985, Infection and immunity.

[4]  T. Yamada,et al.  Effects of oxygen on pyruvate formate-lyase in situ and sugar metabolism of Streptococcus mutans and Streptococcus sanguis , 1985, Infection and immunity.

[5]  R. Khandelwal,et al.  Isolation and Characterization of Intracellular Polysaccharide from Actinomyces viscosus (Short Communication) , 1983 .

[6]  J. Carlsson,et al.  Hydrogen peroxide excretion by oral streptococci and effect of lactoperoxidase-thiocyanate-hydrogen peroxide , 1983, Infection and immunity.

[7]  T. Yamada,et al.  Involvement of oxygen-sensitive pyruvate formate-lyase in mixed-acid fermentation by Streptococcus mutans under strictly anaerobic conditions , 1982, Journal of bacteriology.

[8]  T. Yamada,et al.  Rate-limiting steps of the glycolytic pathway in the oral bacteria Streptococcus mutans and Streptococcus sanguis and the influence of acidic pH on the glucose metabolism. , 1980, Archives of oral biology.

[9]  D. Birkhed,et al.  Glycogen synthesis pathway in Streptococcus mutans strain NCTC 10449S and its glycogen synthesis-defective mutant 805. , 1979, Archives of oral biology.

[10]  O. B. Dirks,et al.  Intracellular polysaccharide metabolism in Streptococcus mutans. , 1978 .

[11]  J. Thompson,et al.  Phosphoenolpyruvate and 2-phosphoglycerate: endogenous energy source(s) for sugar accumulation by starved cells of Streptococcus lactis , 1977, Journal of bacteriology.

[12]  G. J. Walker,et al.  Metabolism of the reserve polysaccharide of Streptococcus mitior (mitis): is there a second alpha-1,4-glucan phosphorylase? , 1976, Journal of bacteriology.

[13]  T. Katayama,et al.  Clinical observation of dental plaque maturation. Application of oxidation-reduction indicator dyes. , 1975, Journal of periodontology.

[14]  M. Freedman,et al.  Variation in internal polysaccharide synthesis among Streptococcus mutans strains , 1975, Infection and immunity.

[15]  C. Saxton.  Determination by electron microscope autoradiography of the distribution in plaque of organisms that synthesize intracellular polysaccharide in situ. , 1975, Caries research.

[16]  G. Shockman,et al.  Measurement of Intracellular Iodophilic Polysaccharide in Two Cariogenic Strains of Streptococcus mutans by Cytochemical and Chemical Methods , 1974, Infection and immunity.

[17]  I. R. Hamilton,et al.  Some regulatory properties of glycogen phosphorylase from Streptococcus salivarius. , 1973, Archives of biochemistry and biophysics.

[18]  B. F. Hammond.  Intracellular polysaccharide production by human oral strains of Lactobacillus casei. , 1971, Archives of oral biology.

[19]  J. van Houte,et al.  Cell wall thickening and intracellular polysaccharide in microorganisms of the dental plaque. , 1971, Caries research.

[20]  J. van Houte,et al.  Synthesis of iodophilic polysaccharide by human oral streptococci. , 1970, Archives of oral biology.

[21]  J. van Houte,et al.  Role of Glycogen in Survival of Streptococcus mitis , 1970, Journal of bacteriology.

[22]  M. Ash,et al.  Oxidation reduction potential of developing plaque, periodontal pockets and gingival sulci. , 1969, Journal of periodontology.

[23]  J. van Houte,et al.  Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci. , 1969, Archives of oral biology.

[24]  J. van Houte,et al.  The iodophilic polysaccharide synthesized by Stretococcus salivarius. , 1968, Caries research.

[25]  J. Houte.  RELATIONSHIP BETWEEN CARBOHYDRATE INTAKE AND POLYSACCHARIDE-STORING MICRO-ORGANISMS IN DENTAL PLAQUE. , 1964 .

[26]  R. Gibbons,et al.  Synthesis of intracellular iodophilic polysaccharide by Streptococcus mitis. , 1963, Archives of oral biology.

[27]  S. Socransky,et al.  Intracellular polysaccharide storage by organisms in dental plaques. Its relation to dental caries and microbial ecology of the oral cavity. , 1962, Archives of oral biology.

[28]  Ennis Layne,et al.  SPECTROPHOTOMETRIC AND TURBIDIMETRIC METHODS FOR MEASURING PROTEINS , 1957 .