Bioorganic and Biocatalytic Reactions

[1]  J C M van Hest,et al.  Enzymatic enantioselective C-C-bond formation in microreactors. , 2008, Biotechnology and bioengineering.

[2]  Yi Zhang,et al.  Clockwork PCR including sample preparation. , 2008, Angewandte Chemie.

[3]  J. Kong,et al.  Efficient proteolysis system: a nanozeolite-derived microreactor. , 2006, Small.

[4]  Andreas Kirschning,et al.  Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix. , 2007, Organic & biomolecular chemistry.

[5]  F Baganz,et al.  Immobilised enzyme microreactor for screening of multi-step bioconversions: characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols. , 2011, Journal of biotechnology.

[6]  Larry J Kricka,et al.  Micropillar array chip for integrated white blood cell isolation and PCR. , 2005, Biomolecular engineering.

[7]  Samuel K Sia,et al.  Effect of volume- and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays. , 2008, Lab on a chip.

[8]  Jonathan S Dordick,et al.  Silica-immobilized enzymes for multi-step synthesis in microfluidic devices. , 2007, Biotechnology and bioengineering.

[9]  Yi Zhang,et al.  Advances in microfluidic PCR for point-of-care infectious disease diagnostics. , 2011, Biotechnology advances.

[10]  Akira Endo,et al.  Microreactor with mesoporous silica support layer for lipase catalyzed enantioselective transesterification , 2010 .

[11]  C. Klapperich,et al.  Sample preparation module for bacterial lysis and isolation of DNA from human urine , 2009, Biomedical microdevices.

[12]  S. Bruckenstein,et al.  Integration of an on-line protein digestion microreactor to a nanoelectrospray emitter for peptide mapping. , 2006, Analytical biochemistry.

[13]  T. Ohmori,et al.  Enzymatic reactions inside a microreactor with a mesoporous silica catalyst support layer , 2009 .

[14]  Shinji Sakai,et al.  Development and Characterization of a Silica Monolith Immobilized Enzyme Micro-bioreactor , 2005 .

[15]  Lihua Zhang,et al.  Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity. , 2008, Analytical chemistry.

[16]  Stephan Mohr,et al.  Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase. , 2010, Lab on a chip.

[17]  H. Ju,et al.  Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay. , 2011, Analytica chimica acta.

[18]  Chunsun Zhang,et al.  PCR microfluidic devices for DNA amplification. , 2006, Biotechnology advances.

[19]  Brian N. Johnson,et al.  An integrated nanoliter DNA analysis device. , 1998, Science.

[20]  Bernd Nidetzky,et al.  Development of a microfluidic immobilised enzyme reactor. , 2007, Chemical communications.

[21]  A. Sinz,et al.  A capillary monolithic trypsin reactor for efficient protein digestion in online and offline coupling to ESI and MALDI mass spectrometry. , 2010, Analytical chemistry.

[22]  E. Wimmer,et al.  Putting Synthesis into Biology: A Viral View of Genetic Engineering through De Novo Gene and Genome Synthesis , 2009, Chemistry & Biology.

[23]  Monica Riley,et al.  Escherichia coli K-12: a cooperatively developed annotation snapshot—2005 , 2006, Nucleic acids research.

[24]  Yukui Zhang,et al.  Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed‐phase liquid chromatography with mass spectrometry for online protein profiling , 2011, Electrophoresis.

[25]  Chulhwan Park,et al.  β-Galactosidase-immobilised microreactor fabricated using a novel technique for enzyme immobilisation and its application for continuous synthesis of lactulose , 2012 .

[26]  E. Tamiya,et al.  Fluorescence-based assay with enzyme amplification on a micro-flow immunosensor chip for monitoring coplanar polychlorinated biphenyls , 2005 .

[27]  Polona Žnidaršič-Plazl,et al.  Modelling of laccase-catalyzed l-DOPA oxidation in a microreactor , 2009 .

[28]  Bingcheng Lin,et al.  Microfluidic device for integrated restriction digestion reaction and resulting DNA fragment analysis , 2008, Electrophoresis.

[29]  R. Zare,et al.  Integration of on-line protein digestion, peptide separation, and protein identification using pepsin-coated photopolymerized sol-gel columns and capillary electrophoresis/mass spectrometry. , 2004, Analytical chemistry.

[30]  Frantisek Foret,et al.  Immobilized microfluidic enzymatic reactors , 2004, Electrophoresis.

[31]  Hideaki Maeda,et al.  Microchannel enzyme reactors and their applications for processing. , 2006, Trends in biotechnology.

[32]  M. N. Kashid,et al.  Microstructured Reactors for Multiphase Reactions: State of the Art , 2009 .

[33]  G. Collins,et al.  Microchip-based electrochemical enzyme immunoassays. , 2007, Methods in molecular biology.

[34]  A. Srinivasan,et al.  Chip‐Based Polyketide Biosynthesis and Functionalization , 2006, Biotechnology progress.

[35]  Il-Hoon Cho,et al.  Plastic ELISA-on-a-chip based on sequential cross-flow chromatography. , 2006, Analytical chemistry.

[36]  L. Locascio,et al.  Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification. , 2001, Analytical chemistry.

[37]  Ana G. Pereira-Medrano,et al.  Rapid fabrication of glass/PDMS hybrid µIMER for high throughput membrane proteomics. , 2010, Lab on a chip.

[38]  Roger A Sheldon,et al.  Immobilised enzymes: carrier-bound or carrier-free? , 2003, Current opinion in biotechnology.

[39]  G. Ramsay DNA chips: State-of-the art , 1998, Nature Biotechnology.

[40]  Xiaoyan Wang,et al.  Chemistry of vegetable physiology and agriculture , 1873 .

[41]  J. Littlechild,et al.  Immobilization of thermophilic enzymes in miniaturized flow reactors. , 2007, Biochemical Society transactions.

[42]  B. Lin,et al.  A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis. , 2010, Analytica chimica acta.

[43]  Yoke Kong Kuan,et al.  Integrated two-step gene synthesis in a microfluidic device. , 2009, Lab on a chip.

[44]  Polona Žnidaršič-Plazl,et al.  Lipase-catalyzed synthesis of isoamyl acetate in an ionic liquid/n-heptane two-phase system at the microreactor scale. , 2009, Lab on a chip.

[45]  Aravind Srinivasan,et al.  Bacterial P450-catalyzed polyketide hydroxylation on a microfluidic platform. , 2004, Biotechnology and bioengineering.

[46]  Peter H Seeberger,et al.  Microreactors as tools for synthetic chemists-the chemists' round-bottomed flask of the 21st century? , 2006, Chemistry.

[47]  Steffen Hardt,et al.  Automated chip-based device for simple and fast nucleic acid amplification , 2005, Expert review of molecular diagnostics.

[48]  Andreas Schmid,et al.  Miniaturizing Biocatalysis: Enzyme‐Catalyzed Reactions in an Aqueous/Organic Segmented Flow Capillary Microreactor , 2011 .

[49]  Ji Ji,et al.  Gold Nanoparticle Assembly Microfluidic Reactor for Efficient On-line Proteolysis*S , 2007, Molecular & Cellular Proteomics.

[50]  Wei Lin,et al.  Design and optimization of porous polymer enzymatic digestors for proteomics. , 2009, Journal of separation science.

[51]  Regine M. Schoenherr,et al.  On‐line protein digestion and peptide mapping by capillary electrophoresis with post‐column labeling for laser‐induced fluorescence detection , 2004, Electrophoresis.

[52]  Polona Žnidaršič-Plazl,et al.  On the feasibility of in situ steroid biotransformation and product recovery in microchannels , 2010 .

[53]  G. Stemme,et al.  Pyrosequencing in a microfluidic flow-through device. , 2005, Analytical chemistry.

[54]  M. Fujii,et al.  Differential regulation of chemical reactions in a microchannel reaction system , 2004 .

[55]  Baohong Liu,et al.  Titania and alumina sol-gel-derived microfluidics enzymatic-reactors for peptide mapping: design, characterization, and performance. , 2004, Journal of proteome research.

[56]  Highly sensitive pyrosequencing system with polymer-supported enzymes for high-throughput DNA analysis. , 2011, Analytical chemistry.

[57]  P. Righetti,et al.  Controlled enzyme-immobilisation on capillaries for microreactors for peptide mapping , 2004, Analytical and bioanalytical chemistry.

[58]  M. Heller DNA microarray technology: devices, systems, and applications. , 2002, Annual review of biomedical engineering.

[59]  Baohong Liu,et al.  Enhancement of proteolysis through the silica‐gel‐derived microfluidic reactor , 2007, Proteomics.

[60]  K. Waldron,et al.  Reproducibility of a solid-phase trypsin microreactor for peptide mapping by capillary electrophoresis , 2000 .

[61]  Peter H. Seeberger,et al.  Microreactor Synthesis of β‐Peptides , 2006 .

[62]  P. Neužil,et al.  Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes , 2006, Nucleic acids research.

[63]  F. Foret,et al.  Liquid phase interfacing and miniaturization in matrix‐assisted laser desorption/ionization mass spectrometry , 2002, Proteomics.

[64]  Jean-Louis Viovy,et al.  Use of self assembled magnetic beads for on-chip protein digestion. , 2005, Lab on a chip.

[65]  Qin Li,et al.  Preparation and evaluation of an enzymatic microreactor based on HILIC matrix for digestion and identification of proteins , 2010 .

[66]  L. Zhang,et al.  Monolith-based immobilized enzyme reactors: recent developments and applications for proteome analysis. , 2007, Journal of separation science.

[67]  A. Jungbauer,et al.  Affinity monoliths generated by in situ polymerization of the ligand. , 2001, Analytical chemistry.

[68]  Pawel L Urban,et al.  Enzymatic microreactors in chemical analysis and kinetic studies. , 2006, Biotechnology advances.

[69]  Gang Chen,et al.  Fiber‐packed channel bioreactor for microfluidic protein digestion , 2007, Proteomics.

[70]  A replaceable microreactor for on-line protein digestion in a two-dimensional capillary electrophoresis system with tandem mass spectrometry detection. , 2011, Journal of chromatography. A.

[71]  Jean-Louis Viovy,et al.  Controlled proteolysis of normal and pathological prion protein in a microfluidic chip. , 2008, Lab on a chip.

[72]  Paul Watts,et al.  The synthesis of peptides using micro reactors , 2001 .

[73]  S. J. Lee,et al.  Micro total analysis system (μ-TAS) in biotechnology , 2004, Applied Microbiology and Biotechnology.

[74]  Remko M Boom,et al.  Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale , 2008, Biotechnology and bioengineering.

[75]  E. Stigter,et al.  Pepsin immobilized in dextran-modified fused-silica capillaries for on-line protein digestion and peptide mapping. , 2008, Analytica chimica acta.

[76]  R. Zare,et al.  Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol gel. , 2005, Analytical chemistry.

[77]  H. Yamaguchi,et al.  Multidigestion in continuous flow tandem protease-immobilized microreactors for proteomic analysis. , 2010, Analytical biochemistry.

[78]  Ji Ji,et al.  Enhanced protein digestion through the confinement of nanozeolite-assembled microchip reactors. , 2008, Analytical chemistry.

[79]  S. Quake,et al.  Multistep Synthesis of a Radiolabeled Imaging Probe Using Integrated Microfluidics , 2005, Science.

[80]  Katsunori Tanaka,et al.  Oligosaccharide Synthesis by Affinity Separation Based on Molecular Recognition between Podand Ether and Ammonium Ion , 2005 .

[81]  Jim-Min Fang,et al.  Flash synthesis of carbohydrate derivatives in chaotic microreactors , 2011 .

[82]  S. Mugo,et al.  The Development of Flow-through Bio-Catalyst Microreactors from Silica Micro Structured Fibers for Lipid Transformations , 2011, Lipids.

[83]  Syed A Hashsham,et al.  Miniaturized nucleic acid amplification systems for rapid and point-of-care diagnostics: a review. , 2012, Analytica chimica acta.

[84]  Stephen R. Quake,et al.  A microfluidic oligonucleotide synthesizer , 2010, Nucleic acids research.

[85]  Jitae Kim,et al.  A PCR reactor with an integrated alumina membrane for nucleic acid isolation. , 2010, The Analyst.

[86]  Stephen R Quake,et al.  Solvent resistant microfluidic DNA synthesizer. , 2007, Lab on a chip.

[87]  Masaru Kato,et al.  Creation of an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip. , 2003, Analytical chemistry.

[88]  Michael G. Roper,et al.  A fully integrated microfluidic genetic analysis system with sample-in–answer-out capability , 2006, Proceedings of the National Academy of Sciences.

[89]  Baohong Liu,et al.  Stable microstructured network for protein patterning on a plastic microfluidic channel: strategy and characterization of on-chip enzyme microreactors. , 2004, Analytical chemistry.

[90]  Frantisek Svec,et al.  Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices. , 2002, Analytical chemistry.

[91]  H. Mao,et al.  Design and characterization of immobilized enzymes in microfluidic systems. , 2002, Analytical chemistry.

[92]  James P Landers,et al.  A microchip-based proteolytic digestion system driven by electroosmotic pumping. , 2003, Lab on a chip.

[93]  William J. Greenleaf,et al.  Fluorogenic DNA Sequencing in PDMS Microreactors , 2011, Nature Methods.

[94]  F. Foret,et al.  Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries. , 2007, Journal of chromatography. A.

[95]  R. Fitzgerald,et al.  Enzymatic debittering of food protein hydrolysates. , 2006, Biotechnology advances.

[96]  Katsunori Tanaka,et al.  Highly Efficient α‐Sialylation by Virtue of Fixed Dipole Effects of N‐Phthalyl Group: Application to Continuous Flow Synthesis of α(2‐3)‐and α(2‐6)‐Neu5Ac‐Gal Motifs by Microreactor , 2007 .

[97]  Paul Watts,et al.  Solution phase synthesis of β-peptides using micro reactors , 2002 .

[98]  Shinji Katsura,et al.  Activation of restriction enzyme by electrochemically released magnesium ion. , 2004, Journal of bioscience and bioengineering.

[99]  Matthew B. Kerby,et al.  Measurements of kinetic parameters in a microfluidic reactor. , 2006, Analytical chemistry.

[100]  Peter H Seeberger,et al.  Oligosaccharide synthesis in microreactors. , 2007, Organic letters.

[101]  H. Zou,et al.  Immobilized metal‐ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ ionization‐time of flight‐mass spectrometry , 2003, Electrophoresis.

[102]  Hideaki Maeda,et al.  Simple method for preparation of nanostructure on microchannel surface and its usage for enzyme-immobilization. , 2003, Chemical communications.

[103]  Steven A. Soper,et al.  Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS , 2008, Journal of the American Society for Mass Spectrometry.

[104]  Derek J. Wilson,et al.  A microfluidic reactor for rapid, low-pressure proteolysis with on-chip electrospray ionization. , 2010, Rapid communications in mass spectrometry : RCM.

[105]  Satoshi Hamakawa,et al.  Immobilization of enzyme-encapsulated nanoporous material in a microreactor and reaction analysis , 2011 .

[106]  Andre Sharon,et al.  Low cost and manufacturable complete microTAS for detecting bacteria. , 2009, Lab on a chip.

[107]  Robin H. Liu,et al.  Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection. , 2004, Analytical chemistry.

[108]  Denz Lee,et al.  Mixing in tangentially crossing microchannels , 2011 .

[109]  Hiroyuki Nakamura,et al.  Immobilization of enzymes on a microchannel surface through cross-linking polymerization. , 2005, Chemical communications.

[110]  Helen C. Hailes,et al.  Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites , 2012 .

[111]  Baohong Liu,et al.  Microchip-based ELISA strategy for the detection of low-level disease biomarker in serum. , 2009, Analytica chimica acta.

[112]  Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor. , 2011, Journal of proteomics.

[113]  Jerome P Ferrance,et al.  A simple, valveless microfluidic sample preparation device for extraction and amplification of DNA from nanoliter-volume samples. , 2006, Analytical chemistry.

[114]  Ping He,et al.  Development of enzyme immobilized monolith micro-reactors integrated with microfluidic electrochemical cell for the evaluation of enzyme kinetics , 2010 .

[115]  B. Ngamsom,et al.  A microreactor for the study of biotransformations by a cross-linked gamma-lactamase enzyme. , 2009, Biotechnology journal.

[116]  A. Srinivasan,et al.  Multienzyme catalysis in microfluidic biochips. , 2003, Biotechnology and bioengineering.

[117]  H. Stone,et al.  Enzymatic reactions in microfluidic devices: Michaelis-Menten kinetics. , 2008, Analytical chemistry.

[118]  Hideaki Maeda,et al.  Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor. , 2002, Lab on a chip.

[119]  Toru Futami,et al.  Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. , 2003, Lab on a chip.

[120]  Baohong Liu,et al.  Multilayer-assembled microchip for enzyme immobilization as reactor toward low-level protein identification. , 2006, Analytical chemistry.

[121]  T. A. Beek,et al.  A DNA-based strategy for dynamic positional enzyme immobilization inside fused silica microchannels , 2011 .

[122]  Simon C. Potter,et al.  A Genome-Wide Association Search for Type 2 Diabetes Genes in African Americans , 2012, PLoS ONE.

[123]  Electrochemical effects related to synthesis in micro reactors operating under electrokinetic flow , 2004 .

[124]  Richard A Mathies,et al.  Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis. , 2003, Current opinion in biotechnology.

[125]  Hideaki Maeda,et al.  Enzymatic Processing in Microfluidic Reactors , 2008, Biotechnology & genetic engineering reviews.

[126]  L. Zhang,et al.  Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis. , 2011, Journal of chromatography. A.

[127]  Takehiko Kitamori,et al.  A micro-ELISA system for the rapid and sensitive measurement of total and specific immunoglobulin E and clinical application to allergy diagnosis. , 2009, Lab on a chip.

[128]  Bernd Nidetzky,et al.  Enzymatic synthesis of β‐glucosylglycerol using a continuous‐flow microreactor containing thermostable β‐glycoside hydrolase CelB immobilized on coated microchannel walls , 2009, Biotechnology and bioengineering.

[129]  Pengyuan Yang,et al.  Trypsin entrapped in poly(diallyldimethylammonium chloride) silica sol-gel microreactor coupled to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. , 2008, Rapid communications in mass spectrometry : RCM.

[130]  Hiroyuki Nakamura,et al.  Integrated microreaction system for optical resolution of racemic amino acids. , 2007, Lab on a chip.

[131]  Hiroyuki Nakamura,et al.  Facile Preparation of an Enzyme‐Immobilized Microreactor using a Cross‐Linking Enzyme Membrane on a Microchannel Surface , 2006 .

[132]  Jing-Tang Yang,et al.  Analysis of chaos and FRET reaction in split-and-recombine microreactors , 2011 .

[133]  Patrick Hunziker,et al.  Modeling and Optimization of High-Sensitivity, Low-Volume Microfluidic-Based Surface Immunoassays , 2005, Biomedical microdevices.

[134]  Xiangmin Zhang,et al.  On-chip enzymatic microreactor using trypsin-immobilized superparamagnetic nanoparticles for highly efficient proteolysis. , 2007, Journal of chromatography. A.

[135]  L. Licklider,et al.  Characterization of reaction dynamics in a trypsin-modified capillary microreactor. , 1998, Analytical chemistry.

[136]  Roland Wohlgemuth,et al.  Biocatalysis--key to sustainable industrial chemistry. , 2010, Current opinion in biotechnology.

[137]  Haojie Lu,et al.  Zeolite nanoparticle modified microchip reactor for efficient protein digestion. , 2006, Lab on a chip.

[138]  M. Przybylski,et al.  Functionalized magnetic micro‐ and nanoparticles: Optimization and application to μ‐chip tryptic digestion , 2006, Electrophoresis.

[139]  Klavs F Jensen,et al.  Microreactor-based reaction optimization in organic chemistry--glycosylation as a challenge. , 2005, Chemical communications.

[140]  H. Schoemaker,et al.  Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate , 2008 .

[141]  M. Fujii,et al.  Enhanced Enzymatic Reactions in a Microchannel Reactor , 2002 .

[142]  Polona Žnidaršič-Plazl,et al.  Modelling and experimental studies on lipase-catalyzed isoamyl acetate synthesis in a microreactor , 2009 .

[143]  Michael V. Pishko,et al.  Immobilization of multi-enzyme microreactors inside microfluidic devices , 2005 .

[144]  Frantisek Svec,et al.  Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions. , 2007, Analytical chemistry.

[145]  Andreas Manz,et al.  Microfluidics: Applications for analytical purposes in chemistry and biochemistry , 2008, Electrophoresis.

[146]  T Laurell,et al.  Integrated microanalytical technology enabling rapid and automated protein identification. , 2000, Analytical chemistry.

[147]  Jing-Tang Yang,et al.  A novel microreactor with 3D rotating flow to boost fluid reaction and mixing of viscous fluids , 2009 .

[148]  G. Seong,et al.  Measurement of enzyme kinetics using a continuous-flow microfluidic system. , 2003, Analytical chemistry.

[149]  Xiaogang Jiang,et al.  Coupling the immobilized trypsin microreactor of monolithic capillary with muRPLC-MS/MS for shotgun proteome analysis. , 2006, Journal of proteome research.

[150]  John M Woodley,et al.  Accelerated design of bioconversion processes using automated microscale processing techniques. , 2003, Trends in biotechnology.

[151]  Samuel M. Mugo,et al.  Lipase immobilized microstructured fiber based flow-through microreactor for facile lipid transformations , 2010 .

[152]  Haojie Lu,et al.  Microfluidic enzymatic-reactors for peptide mapping: strategy, characterization, and performance. , 2004, Lab on a chip.

[153]  Liangliang Sun,et al.  Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electrospray ionization mass spectrometry. , 2006, Journal of chromatography. A.

[154]  G. Whitesides The origins and the future of microfluidics , 2006, Nature.

[155]  Evaluation of cell-free protein synthesis using PDMS-based microreactor arrays. , 2008, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.