Analysis of efficiency of the double- centrifugation protocol to prepare platelet rich plasma (PRP) – an experimental study in rabbits

Introduction and objective: The purpose of this study was to evaluate the concentrations of platelets obtained from platelet rich plasma (PRP) prepared according to the double-centrifugation protocol. Material and methods: Eight adult male rabbits (White New Zealand) weighing 2.8 to 4 kg were used. After general anesthesia, 10 ml of blood were drawn from each animal via cardiac puncture. Each blood sample was centrifuged according to the protocol of Sonnleitner et al. (2000). The peripheral blood (total) from each animal and the PRP samples platelets were counted manually. Data were submitted to statistical analysis. The normality of the data was confirmed and the Student’s t test was applied (p < 0.05). Results: PRP samples presented an average platelet count significantly higher than that of peripheral blood. Conclusion: Within the limits of this study, it was concluded that the doublecentrifugation protocol was adequate to prepare PRP.

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