Photobiomodulation (PBM) promoting wound healing has been demonstrated by many studies. Currently, 630 nm and 810 nm light-emitting diodes (LEDs), as light sources, are frequently used in the treatment of diabetic foot ulcers (DFUs) in clinics. However, the dose–effect relationship of LED-mediated PBM is not fully understood. Furthermore, among the 630[Formula: see text]nm and 810[Formula: see text]nm LEDs, which one gets a better effect on accelerating the wound healing of diabetic ulcers is not clear. The aim of this study is to evaluate and compare the effects of 630[Formula: see text]nm and 810[Formula: see text]nm LED-mediated PBM in wound healing both in vitro and in vivo. Our results showed that both 630[Formula: see text]nm and 810[Formula: see text]nm LED irradiation significantly promoted the proliferation of mouse fibroblast cells (L929) at different light irradiances (1, 5, and 10[Formula: see text]mW/cm[Formula: see text]. The cell proliferation rate increased with the extension of irradiation time (100, 200, and 500[Formula: see text]s), but it decreased when the irradiation time was over 500[Formula: see text]s. Both 630[Formula: see text]nm and 810[Formula: see text]nm LED irradiation (5[Formula: see text]mW/cm[Formula: see text] significantly improved the migration capability of L929 cells. No difference between 630[Formula: see text]nm and 810[Formula: see text]nm LED-mediated PBM in promoting cell proliferation and migration was detected. In vivo results presented that both 630[Formula: see text]nm and 810[Formula: see text]nm LED irradiation promoted the wound healing and the expression of the vascular endothelial growth factor (VEGF) and transforming growth factor (TGF) in the wounded skin of type 2 diabetic mice. Overall, these results suggested that LED-mediated PBM promotes wound healing of diabetic mice through promoting fibroblast cell proliferation, migration, and the expression of growth factors in the wounded skin. LEDs (630[Formula: see text]nm and 810[Formula: see text]nm) have a similar outcome in promoting wound healing of type 2 diabetic mice.