Automated fluorescent in situ hybridization (FISH) analysis of t(9;22)(q34;q11) in interphase nuclei

For chronic myeloid leukemia, the FISH detection of t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes is an alternative method to bone marrow karyotyping for monitoring treatment. With automation, several drawbacks of manual analysis may be circumvented. In this article, the capabilities of a commercially available automated image acquisition and analysis system were determined by detecting t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes.

[1]  J. Rowley.  A New Consistent Chromosomal Abnormality in Chronic Myelogenous Leukaemia identified by Quinacrine Fluorescence and Giemsa Staining , 1973, Nature.

[2]  T. Lörch,et al.  Automatic quantification of gene amplification in clinical samples by IQ‐FISH , 2004, Cytometry. Part B, Clinical cytometry.

[3]  S. McKenna,et al.  Molecular abnormalities in chronic myeloid leukemia: deregulation of cell growth and apoptosis. , 2000, The oncologist.

[4]  M. Lejman,et al.  Fluorescence in situ hybridization BCR/ABL fusion signal rate in interphase nuclei of healthy volunteer donors: a test study for establishing false positive rate. , 2003, Cancer genetics and cytogenetics.

[5]  C. Litz,et al.  Chronic myelogenous leukemia: molecular diagnostic considerations. , 1994, Human pathology.

[6]  S. Advani,et al.  Fluorescence in situ hybridization: a highly efficient technique of molecular diagnosis and predication for disease course in patients with myeloid leukemias. , 2001, Cancer genetics and cytogenetics.

[7]  Philippe Vielh,et al.  Signal Amplification of FISH for Automated Detection Using Image Cytometry , 1997, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[8]  P. Paschka,et al.  Molecular monitoring of response to imatinib (Glivec®) in CML patients pretreated with interferon alpha. Low levels of residual disease are associated with continuous remission , 2003, Leukemia.

[9]  A. Chase,et al.  A two‐color BCR–ABL probe that greatly reduces the false positive and false negative rates for fluorescence in situ hybridization in chronic myeloid leukemia , 1998, Genes, chromosomes & cancer.

[10]  U. Reinhold,et al.  FISH for BCR-ABL on interphases of peripheral blood neutrophils but not of unselected white cells correlates with bone marrow cytogenetics in CML patients treated with imatinib , 2003, Leukemia.

[11]  E. Mendonça,et al.  Assessing HER‐2 status in fresh frozen and archival cytological samples obtained by fine needle aspiration cytology , 2004, Cytopathology : official journal of the British Society for Clinical Cytology.

[12]  M Kozubek,et al.  Combined confocal and wide-field high-resolution cytometry of fluorescent in situ hybridization-stained cells. , 2001, Cytometry.

[13]  K. Nechvíle.  The High Resolution , 2005 .

[14]  J W Gray,et al.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Michal Kozubek,et al.  Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukaemia , 1997, Human Genetics.

[16]  H. Netten,et al.  Automation of spot counting in interphase cytogenetics using brightfield microscopy. , 1996, Cytometry.

[17]  A. Chase,et al.  Lack of reciprocal translocation in BCR-ABL positive Ph-negative chronic myeloid leukaemia. , 1994, Leukemia.

[18]  A D Carothers,et al.  Counting, measuring, and mapping in FISH-labelled cells: sample size considerations and implications for automation. , 1994, Cytometry.

[19]  L. Trakhtenbrot,et al.  Standardization criteria for the detection of BCR/ABL fusion in interphase nuclei of chronic myelogenous leukemia patients by fluorescence in situ hybridization. , 2000, Cancer genetics and cytogenetics.

[20]  R. Naumann,et al.  Problems with interphase fluorescence in situ hybridization in detecting BCR/ABL-positive cells in some patients using a novel technique with extra signals. , 2001, Cancer genetics and cytogenetics.

[21]  M Kozubek,et al.  High-resolution cytometry of FISH dots in interphase cell nuclei. , 1999, Cytometry.

[22]  J. Goldman,et al.  Factors influencing the false positive and negative rates of BCR‐ABL fluorescence in situ hybridization , 1997, Genes, chromosomes & cancer.

[23]  J. Hernández,et al.  Detection of the Mbcr/abl translocation in chronic myeloid leukemia by fluorescence in situ hybridization: comparison with conventional cytogenetics and implications for minimal residual disease detection. , 1997, Human pathology.

[24]  F del Pozo,et al.  Automated FISH spot counting in interphase nuclei: statistical validation and data correction. , 1998, Cytometry.

[25]  S. Schwartz,et al.  A multicenter investigation with D-FISH BCR/ABL1 probes. , 2000, Cancer genetics and cytogenetics.