Surface functionalization of bioactive glasses.

Different cleaning and silanization methods have been applied to bioactive glasses with the aim of covalently bonding bone morphogenetic proteins (BMP-2) to the surface. Several glasses, with different bioactivity index, were cleaned with acidic, basic, or neutral aqueous media to investigate the role of pH in the formation of silanols on glass surfaces of different reactivity. The cleaned glasses were then functionalized using 3-aminopropyl-triethoxysilane (APTS). After the optimization of the silanization procedure, proteins of different complexity were immobilized on the functionalized glasses. To optimize the protein immobilization, a model protein (carnosine) was first used, and the procedure was then used to bind human BMP-2. The glass surfaces were characterized during each step of the treatment by water contact angles and X-ray photoelectron analyses. The APTS functionalization was then used to immobilize bone morphogenetic protein on the bioactive glasses. This result suggested that such a treatment could be successfully used as an efficient alternative to systemic administration of transforming growth factors for the development of local delivery vehicle implants.

[1]  G. Muzio,et al.  Development of glass-ceramic scaffolds for bone tissue engineering: characterisation, proliferation of human osteoblasts and nodule formation. , 2007, Acta biomaterialia.

[2]  Suresh Valiyaveettil,et al.  Developing macroporous bicontinuous materials as scaffolds for tissue engineering. , 2005, Biomaterials.

[3]  Frances S. Ligler,et al.  Comparison of chemical cleaning methods of glass in preparation for silanization , 1999 .

[4]  É. Jallot.  Role of magnesium during spontaneous formation of a calcium phosphate layer at the periphery of a bioactive glass coating doped with MgO , 2003 .

[5]  A. Cormack,et al.  Interaction of water with bioactive glass surfaces , 2006 .

[6]  Y. Tabata,et al.  Enhanced osteoinduction by controlled release of bone morphogenetic protein-2 from biodegradable sponge composed of gelatin and beta-tricalcium phosphate. , 2005, Biomaterials.

[7]  Marc A. Asher,et al.  Iliac Crest Bone Graft Harvest Donor Site Morbidity: A Statistical Evaluation , 1995, Spine.

[8]  G. Muzio,et al.  Biocompatible glass–ceramic materials for bone substitution , 2008, Journal of materials science. Materials in medicine.

[9]  C. D. Wagner,et al.  Auger and photoelectron line energy relationships in aluminum–oxygen and silicon–oxygen compounds , 1982 .

[10]  R Borojevic,et al.  Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. , 2003, Biomaterials.

[11]  Larry L. Hench,et al.  Highly bioactive P2O5–Na2O–CaO–SiO2 glass-ceramics , 2001 .

[12]  T. Kokubo.  Surface chemistry of bioactive glass-ceramics , 1990 .

[13]  Larry L. Hench,et al.  Regeneration of trabecular bone using porous ceramics , 2003 .

[14]  J. Courtney,et al.  Screening of in vitro cytotoxicity by the adhesive film test. , 1990, Biomaterials.

[15]  Hideko Urushihara,et al.  A transcriptional profile of multicellular development in Dictyostelium discoideum. , 2002, Development.

[16]  T. Schiestel,et al.  Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA. , 2000, International journal of pharmaceutics.

[17]  A. Reddi.  Bone Morphogenetic Proteins: From Basic Science to Clinical Applications , 2001, The Journal of bone and joint surgery. American volume.

[18]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[19]  J. Jane,et al.  Bone graft substitutes for the promotion of spinal arthrodesis. , 2001, Neurosurgical focus.

[20]  Banwart Jc,et al.  Iliac crest bone graft harvest donor site morbidity. A statistical evaluation. , 1995 .

[21]  Ning Gu,et al.  Preparation and characterization of magnetite nanoparticles coated by amino silane , 2003 .

[22]  Julian R Jones,et al.  Bioactive sol-gel foams for tissue repair. , 2002, Journal of biomedical materials research.

[23]  A. Monfort,et al.  Adsorption of recombinant human bone morphogenetic protein rhBMP-2m onto hydroxyapatite. , 2005, Journal of inorganic biochemistry.

[24]  J. Vormann.  Magnesium: nutrition and metabolism. , 2003, Molecular aspects of medicine.

[25]  G. Daculsi,et al.  Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. , 1998, Biomaterials.

[26]  J. Pilitsis,et al.  Bone healing and spinal fusion. , 2002, Neurosurgical focus.

[27]  Chikara Ohtsuki,et al.  Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid , 1992 .

[28]  H. Uludaǧ,et al.  Growth Factor Delivery for Bone Tissue Engineering , 2001, Journal of drug targeting.

[29]  Y. M. Lee,et al.  Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices. , 2001, Journal of biomedical materials research.

[30]  E. Verné,et al.  Macroporous bioactive glass-ceramic scaffolds for tissue engineering , 2006, Journal of materials science. Materials in medicine.

[31]  Ilmar Kink,et al.  Preparation of smooth siloxane surfaces for AFM visualization of immobilized biomolecules , 2003 .

[32]  S. W. Kim,et al.  Covalent immobilization of GL-7-ACA acylase on silica gel through silanization , 2002 .

[33]  S. Mann,et al.  The potential of biomimesis in bone tissue engineering: lessons from the design and synthesis of invertebrate skeletons. , 2002, Bone.

[34]  L. Hench.  Sol-gel materials for bioceramic applications , 1997 .

[35]  M. Chapman,et al.  Morbidity at bone graft donor sites. , 1989, Journal of orthopaedic trauma.

[36]  Melba Navarro,et al.  New macroporous calcium phosphate glass ceramic for guided bone regeneration. , 2004, Biomaterials.

[37]  R. Yukna,et al.  Bone replacement grafts. The bone substitutes. , 1998, Dental clinics of North America.

[38]  A. R. Boccaccini,et al.  The surface functionalization of 45S5 Bioglass®-based glass-ceramic scaffolds and its impact on bioactivity , 2006, Journal of materials science. Materials in medicine.

[39]  C. Bianchi,et al.  Early stage reactivity and in vitro behavior of silica-based bioactive glasses and glass-ceramics , 2009, Journal of materials science. Materials in medicine.