COMBINED INOCULATION OF ARBUSCULAR MYCORRHIZAL FUNGI , PSEUDOMONAS FLUORESCENS AND TRICHODERMA SPP . FOR ENHANCING DEFENSE ENZYMES AND YIELD OF THREE PEPPER CULTIVARS

Field experiment was carried out at the experimental station of Szent István University, Gödöllő, Hungary to explore the impact of arbuscular mycorrhizal fungi only alone or together with Trichoderma and plant growth-promoting bacteria on defense enzymes and yield of three pepper varieties. The seven inoculation treatments consisting of arbuscular mycorrhizal fungi (AM), Trichoderma (Tri), plant growth promoting bacteria (Pse) and their combinations (AM+Tri; AM+Tri+Pse; AM+Pse) together with three pepper hybrids and non-inoculation (control) plants were arranged in a randomized complete block design. Defense enzyme activities polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) of various treated plants were measured just before flowering, representing the most sensitive stage of plants. The results showed that AM+Tri+Pse treatment enhanced the yield most among microbial inoculations. Highest yield was recorded in the triple treatment in Karpex cultivar, however, Karpia and Kaptur variety obtained more improved yield by microbial inoculations. Defense enzymes activities generally were most induced in the combination of three inoculants in cultivars whereas different responses in induction of defense enzymes were found in other microbial treatments, depending on specific interactions between microbe and pepper genotype. These results suggested that the triple application brought more benefits to the host plant.

[1]  M. Lorito,et al.  Trichoderma and its secondary metabolites improve yield and quality of grapes , 2017 .

[2]  J. Bakr.  YIELD AND QUALITY OF MYCORRHIZED PROCESSING TOMATO UNDER WATER SCARCITY , 2017 .

[3]  N. Barber,et al.  Effects of arbuscular mycorrhizal fungi on herbivory defense in two Solanum (Solanaceae) species , 2016 .

[4]  Necla Pehlivan,et al.  Trichoderma atroviride ID20G inoculation ameliorates drought stress-induced damages by improving antioxidant defence in maize seedlings , 2016, Acta Physiologiae Plantarum.

[5]  Yuefei Xu,et al.  Effect of arbuscular mycorrhizal fungi inoculation on cold stress-induced oxidative damage in leaves of Elymus nutans Griseb , 2016 .

[6]  Z. Ye,et al.  Effect of Funneliformis mosseae on the growth, cadmium accumulation and antioxidant activities of Solanum nigrum , 2016 .

[7]  S. Patel,et al.  Antioxidant defense response induced by Trichoderma viride against Aspergillus niger Van Tieghem causing collar rot in groundnut (Arachis hypogaea L.). , 2016, Microbial pathogenesis.

[8]  D. Schwarz,et al.  Flavonol Glucoside and Antioxidant Enzyme Biosynthesis Affected by Mycorrhizal Fungi in Various Cultivars of Onion (Allium cepa L.). , 2016, Journal of agricultural and food chemistry.

[9]  U. Chakraborty,et al.  Antioxidative changes in Citrus reticulata L. induced by drought stress and its effect on root colonization by arbuscular mycorrhizal fungi , 2016 .

[10]  A. Hashem,et al.  Alleviation of cadmium stress in Solanum lycopersicum L. by arbuscular mycorrhizal fungi via induction of acquired systemic tolerance , 2015, Saudi journal of biological sciences.

[11]  Q. Shen,et al.  Biological control of tobacco bacterial wilt using Trichoderma harzianum amended bioorganic fertilizer and the arbuscular mycorrhizal fungi Glomus mosseae , 2016 .

[12]  M. Hamidpour,et al.  Biochemical, physiological and antioxidant enzymatic activity responses of pistachio seedlings treated with plant growth promoting rhizobacteria and Zn to salinity stress , 2015, Acta Physiologiae Plantarum.

[13]  R. Rodrigues,et al.  Effects of arbuscular mycorrhizal fungi on Capsicum spp. , 2015, The Journal of Agricultural Science.

[14]  J. M. Ruiz-Lozano,et al.  Arbuscular mycorrhizal symbiosis regulates physiology and performance of Digitaria eriantha plants subjected to abiotic stresses by modulating antioxidant and jasmonate levels , 2015, Mycorrhiza.

[15]  A. Varma,et al.  Bacterial-Mediated Induction of Systemic Tolerance to Salinity with Expression of Stress Alleviating Enzymes in Soybean (Glycine max L. Merrill) , 2015, Journal of Plant Growth Regulation.

[16]  K. Yadav,et al.  Impact of Arbuscular Mycorrhizal Fungi with Trichoderma viride and Pseudomonas fluorescens on Growth, Yield and Oil Content in Helianthus annuus L. , 2015 .

[17]  S. Nadeem,et al.  Improving the productivity of cucumber through combined application of organic fertilizers and Pseudomonas fluorescens. , 2015 .

[18]  A. Moradi,et al.  Effects of plant growth-promoting rhizobacterium (PGPR) and arbuscular mycorrhizal fungus (AMF) on antioxidant enzyme activities in salt-stressed bean (phaseolus vulgaris l.) , 2014 .

[19]  A. A. Abdel Latef,et al.  Does Inoculation with Glomus mosseae Improve Salt Tolerance in Pepper Plants? , 2014, Journal of Plant Growth Regulation.

[20]  E. F. Abd_Allah,et al.  Mycorrhizal Association and ROS in Plants , 2014 .

[21]  M. Ahemad,et al.  Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective , 2014 .

[22]  A. Latef.  Growth and Some Physiological Activities of Pepper (Capsicum annuum L.) in Response to Cadmium Stress and Mycorrhizal Symbiosis , 2013 .

[23]  A. Gupta,et al.  Arbuscular mycorrhizal inoculation and super phosphate application influence plant growth and yield of Capsicum annuum , 2013 .

[24]  M. Pozo,et al.  Mycorrhiza-Induced Resistance and Priming of Plant Defenses , 2012, Journal of Chemical Ecology.

[25]  J. Albrechtová,et al.  Arbuscular Mycorrhizal Inoculant Increases Yield of Spice Pepper and Affects the Indigenous Fungal Community in the Field , 2012 .

[26]  P. López,et al.  Chemical study and anti-inflammatory activity of Capsicum chacoense and C. baccatum , 2012 .

[27]  I. Ortas,et al.  Effects of arbuscular mycorrhizal inoculation on biochemical parameters in Capsicum annuum grown under long term salt stress , 2012, Turkish Journal of Botany.

[28]  S. Boonlue,et al.  Diversity and efficiency of arbuscular mycorrhizal fungi in soils from organic chili (Capsicum frutescens) farms , 2012, Mycoscience.

[29]  P. Pospíšil.  Molecular mechanisms of production and scavenging of reactive oxygen species by photosystem II. , 2012, Biochimica et biophysica acta.

[30]  K. R. Reddy,et al.  Screening Ornamental Pepper Cultivars for Temperature Tolerance Using Pollen and Physiological Parameters , 2011 .

[31]  N. Tuteja,et al.  Unraveling the role of fungal symbionts in plant abiotic stress tolerance , 2011, Plant signaling & behavior.

[32]  R. Rashid,et al.  Growth, yield and fruit quality of sweet pepper hybrid SH-SP-5 (Capsicum annuum L.) as affected by integration of inorganic fertilizers and organic manures (FYM). , 2011 .

[33]  I. Fernández,et al.  Hormonal and transcriptional profiles highlight common and differential host responses to arbuscular mycorrhizal fungi and the regulation of the oxylipin pathway , 2010, Journal of experimental botany.

[34]  A. Khalid,et al.  Evaluation of arbuscular mycorrhizal fungus, fluorescent Pseudomonas and Trichoderma harzianum formulation against Fusarium oxysporum f. sp. lycopersici for the management of tomato wilt , 2010 .

[35]  Xinghuan Wang,et al.  Capsaicin mediates cell death in bladder cancer T24 cells through reactive oxygen species production and mitochondrial depolarization. , 2010, Urology.

[36]  P. Perkins-Veazie,et al.  Yield and Nutrient Content of Bell Pepper Pods from Plants Developed from Seedlings Inoculated, or Not, with Microorganisms , 2010 .

[37]  B. Lugtenberg,et al.  Plant-growth-promoting rhizobacteria. , 2009, Annual review of microbiology.

[38]  A. Roldán,et al.  Interactions between arbuscular mycorrhizal fungi and Trichoderma harzianum and their effects on Fusarium wilt in melon plants grown in seedling nurseries , 2009 .

[39]  C. Kaya,et al.  The influence of arbuscular mycorrhizal colonisation on key growth parameters and fruit yield of pepper plants grown at high salinity , 2009 .

[40]  M. Kubota,et al.  Interactions between the arbuscular mycorrhizal fungus Glomus mosseae and plant growth-promoting fungi and their significance for enhancing plant growth and suppressing damping-off of cucumber (Cucumis sativus L.) , 2009 .

[41]  M. Polovnikova,et al.  Activities of antioxidant system components and polyphenol oxidase in ontogeny of lawn grasses under megapolis conditions , 2008, Russian Journal of Plant Physiology.

[42]  A. Roldán,et al.  Plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungi modify alleviation biochemical mechanisms in water-stressed plants. , 2008, Functional plant biology : FPB.

[43]  M. Kubota,et al.  EFFECTS OF INTERACTIONS OF ARBUSCULAR MYCORRHIZAL FUNGI AND BENEFICIAL SAPROPHYTIC MYCOFLORA ON PLANT GROWTH AND DISEASE PROTECTION , 2008 .

[44]  S. Demir,et al.  Responses of some different pepper (Capsicum annuum L.) genotypes to inoculation with two different arbuscular mycorrhizal fungi , 2007 .

[45]  A. Mayer.  Polyphenol Oxidases in Plants and Fungi: Going Places? A Review , 2007 .

[46]  V. Russo.  Biological Amendment, Fertilizer Rate, and Irrigation Frequency for Organic Bell Pepper Transplant Production , 2006 .

[47]  S. Altintas,et al.  Effects of Trichoderma harzianum on the yield and fruit quality of tomato plants (Lycopersicon esculentum) grown in an unheated greenhouse , 2006 .

[48]  C. Foyer,et al.  Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context , 2005 .

[49]  Y. Elad.  Mechanisms involved in the biological control ofBotrytis cinerea incited diseases , 1996, European Journal of Plant Pathology.

[50]  H. Hirt,et al.  Reactive oxygen species: metabolism, oxidative stress, and signal transduction. , 2004, Annual review of plant biology.

[51]  B. Siegel.  Plant peroxidases—an organismic perspective , 1993, Plant Growth Regulation.

[52]  A. Pardo,et al.  Interactions between Trichoderma pseudokoningii strains and the arbuscular mycorrhizal fungi Glomus mosseae and Gigaspora rosea , 2004, Mycorrhiza.

[53]  Lenwood S Heath,et al.  Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. , 2002, Journal of experimental botany.

[54]  M. Jiang,et al.  Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. , 2001, Plant & cell physiology.

[55]  P. Olsson,et al.  Suppression of the Biocontrol AgentTrichoderma harzianum by Mycelium of the Arbuscular Mycorrhizal Fungus Glomus intraradices in Root-Free Soil , 1999, Applied and Environmental Microbiology.

[56]  H. Vierheilig,et al.  Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi , 1998, Applied and Environmental Microbiology.

[57]  R. Hammerschmidt,et al.  Transformation of potato with cucumber peroxidase: expression and disease response , 1998 .

[58]  A. Gaur,et al.  A comparison of AM fungi inoculants using Capsicum and Polianthes in marginal soil amended with organic matter , 1998, Mycorrhiza.

[59]  J. Barea,et al.  Applying mycorrhiza biotechnology to horticulture: significance and potentials , 1997 .

[60]  Z. Siddiqui,et al.  Effect of Heterodera cajani, Meloidogyne incognita and Fusarium udum on the wilt disease complex of pigeonpea , 1996 .

[61]  Y. Elad,et al.  Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity , 1996 .

[62]  L. Datnoff,et al.  Biological Control of Fusarium Crown and Root Rot of Tomato in Florida Using Trichoderma harzianum and Glomus intraradices , 1995 .

[63]  A. Godeas,et al.  In vitro interactions between Trichoderma koningii, Fusarium solani and Glomus mosseae , 1994 .

[64]  X. Ye,et al.  Detection of several enzymatic activities in leaf prints of cucumber plants , 1993 .

[65]  I. Fridovich,et al.  Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. , 1987, Analytical biochemistry.

[66]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[67]  Manuela Giovannetti,et al.  AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS , 1980 .

[68]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[69]  L. Sequeira,et al.  Soluble peroxidase in fluid from the intercellular spaces of tobacco leaves. , 1974, Plant physiology.