Biomimetic tissue-engineered systems for advancing cancer research: NCI Strategic Workshop report.

Advanced technologies and biomaterials developed for tissue engineering and regenerative medicine present tractable biomimetic systems with potential applications for cancer research. Recently, the National Cancer Institute convened a Strategic Workshop to explore the use of tissue biomanufacturing for development of dynamic, physiologically relevant in vitro and ex vivo biomimetic systems to study cancer biology and drug efficacy. The workshop provided a forum to identify current progress, research gaps, and necessary steps to advance the field. Opportunities discussed included development of tumor biomimetic systems with an emphasis on reproducibility and validation of new biomimetic tumor models, as described in this report.

[1]  R. Kamm,et al.  In Vitro Model of Tumor Cell Extravasation , 2013, PloS one.

[2]  D. Ingber,et al.  Reconstituting Organ-Level Lung Functions on a Chip , 2010, Science.

[3]  Miles A. Miller,et al.  Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis. , 2013, Journal of the American Chemical Society.

[4]  Kristin M. Fabre,et al.  The National Institutes of Health Microphysiological Systems Program focuses on a critical challenge in the drug discovery pipeline , 2013, Stem Cell Research & Therapy.

[5]  Paul J. A. Kenis,et al.  Microfluidic Generation of Gradient Hydrogels to Modulate Hematopoietic Stem Cell Culture Environment , 2014, Advanced healthcare materials.

[6]  Sanjay Kumar,et al.  The mechanical rigidity of the extracellular matrix regulates the structure, motility, and proliferation of glioma cells. , 2009, Cancer research.

[7]  B. Harley,et al.  Impact of the biophysical features of a 3D gelatin microenvironment on glioblastoma malignancy. , 2013, Journal of biomedical materials research. Part A.

[8]  D. Ingber,et al.  Breast cancer normalization induced by embryonic mesenchyme is mediated by extracellular matrix biglycan. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[9]  Jason R Spence,et al.  How to make an intestine , 2014, Development.

[10]  M. Swartz,et al.  Modeling tumor microenvironments in vitro. , 2014, Journal of biomechanical engineering.

[11]  Sharon Gerecht,et al.  Engineering approaches for investigating tumor angiogenesis: exploiting the role of the extracellular matrix. , 2012, Cancer research.

[12]  D. Branstetter,et al.  RANKL inhibition decreases the incidence of mammary adenocarcinomas in wild type (WT) and MMTV-RANK transgenic mice. , 2009 .

[13]  Alan Wells,et al.  A microphysiological system model of therapy for liver micrometastases , 2014, Experimental biology and medicine.

[14]  Yu-Hsiang Hsu,et al.  In vitro perfused human capillary networks. , 2013, Tissue engineering. Part C, Methods.

[15]  Sanjay Kumar,et al.  CD44-Mediated Adhesion to Hyaluronic Acid Contributes to Mechanosensing and Invasive Motility , 2014, Molecular Cancer Research.

[16]  Sanjay Kumar,et al.  Transforming potential and matrix stiffness co-regulate confinement sensitivity of tumor cell migration. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[17]  Pedro M. Baptista,et al.  The use of whole organ decellularization for the generation of a vascularized liver organoid , 2011, Hepatology.

[18]  Sanjay Kumar,et al.  Elucidating the mechanobiology of malignant brain tumors using a brain matrix-mimetic hyaluronic acid hydrogel platform. , 2011, Biomaterials.

[19]  Nobutaka Hattori,et al.  Cerebral organoids model human brain development and microcephaly , 2014, Movement disorders : official journal of the Movement Disorder Society.

[20]  Mireia Alemany-Ribes,et al.  Bioengineering 3D environments for cancer models. , 2014, Advanced drug delivery reviews.

[21]  M. Waterman,et al.  A three-dimensional in vitro model of tumor cell intravasation. , 2014, Integrative biology : quantitative biosciences from nano to macro.

[22]  Andrew J. Ewald,et al.  Collective Invasion in Breast Cancer Requires a Conserved Basal Epithelial Program , 2013, Cell.

[23]  Peter Molnar,et al.  Microphysiological systems and low-cost microfluidic platform with analytics , 2013, Stem Cell Research & Therapy.

[24]  Olivier Gevaert,et al.  Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture , 2014, Nature Medicine.

[25]  A. Mikos,et al.  Modeling Ewing sarcoma tumors in vitro with 3D scaffolds , 2013, Proceedings of the National Academy of Sciences.

[26]  Scott A. Guelcher,et al.  Matrix Rigidity Induces Osteolytic Gene Expression of Metastatic Breast Cancer Cells , 2010, PloS one.

[27]  Donald E Ingber,et al.  Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[28]  Calvin J Kuo,et al.  Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche , 2009, Nature Medicine.

[29]  Paolo De Coppi,et al.  Production and Implantation of Renal Extracellular Matrix Scaffolds From Porcine Kidneys as a Platform for Renal Bioengineering Investigations , 2012, Annals of surgery.

[30]  Duc-Huy T Nguyen,et al.  Biomimetic model to reconstitute angiogenic sprouting morphogenesis in vitro , 2013, Proceedings of the National Academy of Sciences.

[31]  Brendon M. Baker,et al.  Microfluidics embedded within extracellular matrix to define vascular architectures and pattern diffusive gradients. , 2013, Lab on a chip.

[32]  Linda G. Griffith,et al.  Molecular Network Analysis of Endometriosis Reveals a Role for c-Jun–Regulated Macrophage Activation , 2014, Science Translational Medicine.

[33]  Andrew J Ewald,et al.  ECM microenvironment regulates collective migration and local dissemination in normal and malignant mammary epithelium , 2012, Proceedings of the National Academy of Sciences.

[34]  G. Vunjak‐Novakovic,et al.  Bioengineered human tumor within a bone niche. , 2014, Biomaterials.