Measurement of growth and viability of cells in culture.

Publisher Summary This chapter focuses on cell culture measurements that can be divided into four major categories: (1) visual methods, which employ the use of light microscopy and devices commonly used in hematology; (2) chemical methods, which employ commonly used analytical biochemical procedures adapted to tissue culture; (3) electronic systems using flow-through cells or apertures for measurement of incorporated dyes or cell numbers; and (4) an array of miscellaneous procedures. The handling of cells prior to analysis is extremely important for a variety of reasons: (1) the exogenous growth media may contain components that are being assayed in the cell, (2) leakage of cellular components may occur if cellular integrity is lost at this point, (3) loss of cells may result from severe mechanical handling, and (4) the use of hydrolytic enzymes may lead to the loss of measurable materials.

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