Morphological changes of the pancreas in course of acute pancreatitis during treatment with Ulinastatin.

UNLABELLED Acute pancreatitis is a severe clinical conditio that causes significant mortality in patients. Since we do not have at the moment effective causal treatment research on the use of pro tease inhibitors can produce tangible benefits. In view of the growing number of cases and high mortality in severe AP with one hand, and the lack of a usal treatment research efforts undertaken to search for effective drugs for this disease seem to have deep reasons. AIM OF THE STUDY was to determine the histopathological changes in the pancreas in the treatment of acute pancreatitis with Ulinastatin. MATERIAL AND METHODS The study was conducted in male Wistar rats weighing 250-300 grams. 150 individuals were used for the experiment, 60 of them were treated with Ulinastatin. Experimental acute pancreatitis was induced by the model proposed by Aho and Henckel using sodium taurocholate. Ulinastatin dose numer depended on the duration of the experiment. For histopathological examination pancreatic fragments weighing approximately 1 g each were taken. Assessment and documentation of histopathological preparations were made by light microscopy. RESULTS Evaluation of the histological preparations of various time groups showed significantly improved results after application of Ulinastatin, depending on the duration of the inflammation and the number of doses of the drug. CONCLUSIONS Application for the treatment of UTI leads to inhibition of the inflammatory process at the stage of pancreatic edema and in cases of severe necrotizing course limits the progression of the disease which gives grounds for its clinical use in humans.

[1]  M. Hagiwara,et al.  Continuous intraarterial infusion of protease inhibitors in acute pancreatitis. , 2001, Drugs of today.

[2]  Å. Lasson,et al.  Proteases and protease inhibitors in cerulein-induced acute pancreatitis in rats. , 1999, The Journal of surgical research.

[3]  S. Matsuno,et al.  Role of Early Continuous Regional Arterial Infusion of Protease Inhibitor and Antibiotic in Nonsurgical Treatment of Acute Necrotizing Pancreatitis , 1999, Digestion.

[4]  S. Kondo,et al.  Identification and characterization of the cell-associated binding protein for urinary trypsin inhibitor. , 1998, Biochimica et biophysica acta.

[5]  B. Rau,et al.  Natural course of acute pancreatitis , 1997, World Journal of Surgery.

[6]  H. Kobayashi,et al.  Urinary trypsin inhibitor efficiently inhibits urokinase production in tumor necrosis factor-stimulated cells. , 1996, European Journal of Cell Biology.

[7]  T. Muto,et al.  Effects of the trypsin inhibitor urinastatin on taurocholate-induced pancreatic cellular damage in rats , 1996 .

[8]  M. Tokui,et al.  [A case of severe acute pancreatitis successfully treated by continuous arterial infusion of a protease inhibitor]. , 1994, Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology.

[9]  G. Letko,et al.  Experimental acute pancreatitis--a quantification of dynamics at enzymic and histomorphologic levels. , 1989, Pathology, research and practice.

[10]  S. Matsuno,et al.  Continuous arterial infusion of protease inhibitor for severe acute pancreatitis , 1989, Gastroenterologia Japonica.

[11]  M. Otsuki,et al.  The Protective Effect of the Trypsin Inhibitor Urinastatin on Cerulein‐Induced Acute Pancreatitis in Rats , 1988, Pancreas.

[12]  R. Maciejewski,et al.  Elastase activity and histological changes in lungs and in pancreas due to experimental pancreatitis. , 1999, Folia histochemica et cytobiologica.

[13]  T. Nevalainen,et al.  Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis. , 1980, Scandinavian journal of gastroenterology.

[14]  T. Nevalainen,et al.  Experimental pancreatitis in the rat. Ultrastructure of sodium taurocholate-induced pancreatic lesions. , 1980, Scandinavian journal of gastroenterology.