Differential heat shock protein overexpression and its clinical relevance in systemic lupus erythematosus.

OBJECTIVES--To determine which heat shock proteins (hsps) are overexpressed in systemic lupus erythematosus (SLE), and to examine the relevance of these findings to clinical disease activity. METHODS--Hsp levels in peripheral blood mononuclear cells (PBMC) of patients with SLE and normal controls were measured. Levels were analysed with respect to detailed clinical activity scores. Other hsps were also quantified in 30-50% of these samples. RESULTS--There was significant increase of the 90 kilodalton heat shock protein (hsp90) in patients with SLE and active neuropsychiatric (p < 0.005) and cardiorespiratory (p < 0.01) disease. There was also significant increase of the inducible 72 kilodalton member (hsp72), but not the constitutive 73 kilodalton member (hsp73) of the hsp70 family, and no increase of the 60 kilodalton hsp (hsp60) was seen in patients compared with controls. There was no association of hsp72 with disease activity, and no correlation between hsp90 and hsp72 levels was seen in individual patients. CONCLUSION--There may a specific role for hsp90 in distinct, clinically active subsets of patients with SLE.

[1]  S. Kaufmann.  Heat shock proteins and immune response. , 2011, Current topics in microbiology and immunology.

[2]  D. Isenberg,et al.  Surface expression of heat shock protein 90 by blood mononuclear cells from patients with systemic lupus erythematosus. , 1992, Journal of autoimmunity.

[3]  C. Bombardier,et al.  Crosscultural validation and reliability of 3 disease activity indices in systemic lupus erythematosus. , 1992, The Journal of rheumatology.

[4]  E. Appella,et al.  Mapping of the mouse 86-kDa heat-shock protein expressed gene (Hsp86-1) on chromosome 12 and related genes on chromosomes 3, 4, 9, and 11. , 1991, Genomics.

[5]  J. Winfield,et al.  Do Stress Proteins Play a Role in Arthritis and Autoimmunity? , 1991, Immunological reviews.

[6]  S. Kishimoto,et al.  Enhanced expression of the heat shock protein gene in peripheral blood mononuclear cells of patients with active systemic lupus erythematosus. , 1990, Annals of the rheumatic diseases.

[7]  E. Margoliash,et al.  A peptide binding protein having a role in antigen presentation is a member of the HSP70 heat shock family , 1989, The Journal of experimental medicine.

[8]  R. Scott,et al.  Loss of proliferative potential during terminal differentiation coincides with the decreased abundance of a subset of heterogeneous ribonuclear proteins , 1989, The Journal of cell biology.

[9]  E. Palmer,et al.  Stimulation of a major subset of lymphocytes expressing T cell receptor γδ by an antigen derived from mycobacterium tuberculosis , 1989, Cell.

[10]  D. Isenberg,et al.  Elevated levels of the 90 kd heat shock protein in a proportion of SLE patients with active disease. , 1989, Journal of autoimmunity.

[11]  I. Dunham,et al.  Human major histocompatibility complex contains genes for the major heat shock protein HSP70. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D. Symmons,et al.  Development and Assessment of a Computerized Index of Clinical Disease Activity in Systemic Lupus Erythematosus , 1988 .

[13]  L. Poellinger,et al.  Requirement of hormone for thermal conversion of the glucocorticoid receptor to a DNA-binding state , 1988, Nature.

[14]  R. Young,et al.  Stress proteins are immune targets in leprosy and tuberculosis. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Peterson,et al.  Mitogen activation induces the enhanced synthesis of two heat-shock proteins in human lymphocytes , 1988, Journal of Cell Biology.

[16]  E. Engleman,et al.  T cell regulatory disturbances in the rheumatic diseases. , 1987, Rheumatic diseases clinics of North America.

[17]  S. Kishimoto,et al.  Heat-shock protein synthesis by human peripheral mononuclear cells from SLE patients. , 1987, Biochemical and biophysical research communications.

[18]  E. Craig,et al.  Eukaryotic Mr 83,000 heat shock protein has a homologue in Escherichia coli. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[19]  D. Toft,et al.  Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species. , 1985, Biochemistry.

[20]  D. Isenberg,et al.  The use of C3d as a means of monitoring clinical activity in systemic lupus erythematosus and rheumatoid arthritis. , 1983, Annals of the rheumatic diseases.

[21]  J F Fries,et al.  The 1982 revised criteria for the classification of systemic lupus erythematosus. , 1982, Arthritis and rheumatism.

[22]  A. Todd-Pokropek,et al.  Useful laboratory measurements in the management of systemic lupus erythematosus. , 1982, The Quarterly journal of medicine.

[23]  D. Isenberg,et al.  Transcription of the genes encoding the small heat shock protein ubiquitin is unchanged in patients with systemic lupus erythematosus. , 1992, Autoimmunity.

[24]  T. Lehner,et al.  Expression of the 90 kD heat shock protein in Behçet's syndrome. , 1992, Autoimmunity.

[25]  R. Young,et al.  Stress proteins and immunology. , 1990, Annual review of immunology.

[26]  E. Palmer,et al.  Stimulation of a major subset of lymphocytes expressing T cell receptor gamma delta by an antigen derived from Mycobacterium tuberculosis. , 1989, Cell.

[27]  H Pelham,et al.  Heat-shock proteins. Coming in from the cold. , 1988, Nature.

[28]  E. Craig,et al.  The heat shock response. , 1985, CRC critical reviews in biochemistry.

[29]  E. Baulieu,et al.  Common non-hormone binding component in non-transformed chick oviduct receptors of four steroid hormones , 1984, Nature.

[30]  E. Tan,et al.  Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. , 1982, Advances in immunology.