ISOLATION, PRODUCTION AND CHARACTERIZATION OF EXTRACELLULAR PECTINASE FROM Aspergillus niger K3

A pectinase producing fungus was isolated from compost soil. Morphological characteristics, microscopic observations and molecular characterization based on internal transcribed spacer (ITS) sequences led to its identification as Aspergillus niger (GenBank Accession Number: JX556221) and it was designated as Aspergillus niger strain K3. In the present study, the culture condition of the media was found to have a marked effect on pectinase production. The optimum pH and temperature for maximum pectinase activity was 8.0 (70U/ml) and 45C (90U/ml), respectively. The best carbon sources that induce pectinase production was found to be fructose (40U/ml) followed by maltose (38U/ml). Among the nitrogen sources, ammonium nitrate (80U/ml) produced maximum pectinase followed by peptone (72U/ml).

[1]  D. Rao,et al.  SCREENING OF A PECTINOLYTIC FUNGAL STRAIN; Aspergillus foetidus MTCC 10367 FOR THE PRODUCTION OF MULTIPLE ENZYMES OF INDUSTRIAL IMPORTANCE , 2013 .

[2]  S. D. Rajmane,et al.  Impact of carbon and nitrogen sources on pectinase production of post-harvest fungi. , 2012 .

[3]  R. Vijay,et al.  PRODUCTION AND OPTIMIZATION OF EXTRA CELLULAR PROTEASE FROM BACILLUS SP. ISOLATED FROM SOIL , 2012 .

[4]  A. Azad,et al.  Production of Pectinase by Aspergillus niger Cultured in Solid State Media , 2011 .

[5]  E. A. Fedorova,et al.  Isolation and characterization of extracellular pectin lyase from Penicillium canescens , 2007, Biochemistry (Moscow).

[6]  C. Tsai,et al.  Purification and Characterization , 2006 .

[7]  U. Phutela,et al.  Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels , 2005 .

[8]  K. Swaminathan,et al.  Improvement of tea leaves fermentation with Aspergillus spp. pectinase. , 2002, Journal of bioscience and bioengineering.

[9]  C. Webb,et al.  Utilisation of whole wheat flour for the production of extracellular pectinases by some fungal strains , 2001 .

[10]  D R Kashyap,et al.  Applications of pectinases in the commercial sector: a review. , 2001, Bioresource technology.

[11]  A. Pilosof,et al.  Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation , 1999 .

[12]  K. Domsch,et al.  Compendium of Soil Fungi , 1995 .

[13]  D. Tempest,et al.  Partial purification and properties of pectin lyase from Penicillium expansum , 1993, World journal of microbiology & biotechnology.

[14]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[15]  S. Nagai,et al.  Production of xylan-degrading enzymes by thermophilic fungi, Aspergillus fumigatus and Humicola lanuginosa , 1984 .

[16]  M. Taylor,et al.  Applications of microbial enzymes in food systems and in biotechnology. , 1979, Advances in applied microbiology.