High-speed multispectral confocal biomedical imaging

Abstract. A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

[1]  G. Carver.  Scanned photoluminescence with high spatial resolution in semi-insulating GaAs and InP:aspects of surface passivation and photodegradation , 1992 .

[2]  G. Astfalk,et al.  Wafer level testing for semiconductor laser manufacture via spatially resolved photoluminescence , 1995 .

[3]  Tom Kamiel Magda Vercauteren,et al.  In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy. , 2007, American journal of respiratory and critical care medicine.

[4]  Urs Utzinger,et al.  Spectral background and transmission characteristics of fiber optic imaging bundles. , 2008, Applied optics.

[5]  Daniel L. Farkas,et al.  Visible wavelength fiber Bragg grating arrays for high speed biomedical spectral sensing , 2010 .

[6]  John E. Midwinter,et al.  Optical fibers for transmission , 1979 .

[7]  A Thomas Harris,et al.  Spectral mapping tools from the earth sciences applied to spectral microscopy data , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[8]  Daniel L. Farkas,et al.  High-speed multispectral confocal imaging , 2013, Photonics West - Biomedical Optics.

[9]  Richard M. Levenson,et al.  Optical Image Acquisition, Analysis and Processing for Biomedical Applications , 1997, ICIAP.

[10]  Cheong Hoong Diong,et al.  Application of laser-induced autofluorescence spectra detection in human colorectal cancer screening , 2003, European Conference on Biomedical Optics.

[11]  C Cremer,et al.  Chromosome territories, interchromatin domain compartment, and nuclear matrix: an integrated view of the functional nuclear architecture. , 2000, Critical reviews in eukaryotic gene expression.

[12]  D. Farkas,et al.  Non-invasive, Contrast-enhanced Spectral Imaging of Breast Cancer Signatures in Preclinical Animal Models In vivo. , 2010, Journal of cell science & therapy.

[13]  Sebastian Wachsmann-Hogiu,et al.  Advanced optical imaging requiring no contrast agents--a new armamentarium for medicine and surgery. , 2005, Current surgery.

[14]  J. Pawley,et al.  Handbook of Biological Confocal Microscopy , 1990, Springer US.

[15]  A. Vogel,et al.  Mechanisms of pulsed laser ablation of biological tissues. , 2003, Chemical reviews.

[16]  M. Zellweger,et al.  Absolute autofluorescence spectra of human healthy, metaplastic, and early cancerous bronchial tissue in vivo. , 2001, Applied optics.